Showing: 521 - 540 of 581 results
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Plasmid#209340PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mNeonGreen fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
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OmpA-mNG-mCh
Plasmid#124216PurposePeriplasmic OM bound mNeongreen mCherry tandem, ~16 % energy transfer. pNM004 - pTHV037-OmpA-SA1-177-(SA-1)-LEDPPAEL-mNG-mChDepositorInsertOmpA-mNG-mCh
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
OmpA-mNG-sfTq2ox
Plasmid#124220PurposePeriplasmic OM bound mNeongreen superfolder mTurquoise2 ox tandem, ~40 % energy transfer. pNM092 - pTHV037-Ompa-SA1-177-(SA-1)-LEDPPAEL-mNG-sfTq2ox,DepositorInsertOmpA-mNG-sfTq2ox
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSK247
Plasmid#131148Purposedual yTRAP, Gateway destination vector of aTF2 fusion reporting on mNeonGreenDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterpSUP35, min pCYC1AvailabilityAcademic Institutions and Nonprofits only -
Double UP mNeon to mScarlet Rac1-V12
Plasmid#125136PurposeNatively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-V12 (Constitutively Active).DepositorInsertmNeon/mScarletP2A Rac1-V12
UseTagsExpressionMammalianMutationRemoved Not1 site in mScarlet. 12th AA in Rac1 ch…PromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
Double UP mNeon to mScarlet Rac1-N17
Plasmid#125137PurposeNatively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-N17 (Dominant Negative).DepositorInsertmNeon/mScarletP2A Rac1-N17
UseTagsExpressionMammalianMutationRemoved Not1 site in mScarlet. 17th AA in Rac1 ch…PromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pSK221
Plasmid#129249Purposereverse yTRAP, tetR-responsive Neon reporterDepositorInsertpADH1(tetO2)-mNeonGreen
UseTagsExpressionYeastMutationPromoterADH1AvailabilityAcademic Institutions and Nonprofits only -
pGAN213
Plasmid#129270PurposeControl for measuring background levels of yTRAP reporter proteinDepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromotermin pCYC1AvailabilityAcademic Institutions and Nonprofits only -
pDV24_AAV2_DART-VADAR_iRFP720-sensor
Plasmid#200411PurposeAAV vector encoding a DART-VADAR sensor for iRFP720 mRNADepositorInsertDART VADAR sensor
UseAAV and Synthetic BiologyTagsMCP-ADAR2dd(E488Q)-NES, TagBFP, and mNeonGreenExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCAT003
Plasmid#171628Purpose2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreenDepositorInsertsspyCas9 sgRNA-TRAC
mNeon
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCAG and EF1-aAvailabilityAcademic Institutions and Nonprofits only -
pCAT005
Plasmid#171632Purpose2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreenDepositorInsertsspyCas9 sgRNA-B2M
mNeon
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1-a and U6AvailabilityAcademic Institutions and Nonprofits only -
pJRH623
Plasmid#171633Purpose2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreenDepositorInsertsspyCas9 sgRNA-non-targeting
mNeon
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1-a and U6AvailabilityAcademic Institutions and Nonprofits only -
pSK226
Plasmid#129268Purposedual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with mNeonGreen reporterDepositorInsertRnq1
UseTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDV06.2c_DART-VADAR_iRFP720-sensor
Plasmid#200410PurposepDV06 with iRFP720-targeted sensor sequence inserted. Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon detection of the mRNA produced by pDV05.0, pDV05.1 and pDV05.2DepositorInsertClosed-loop DART VADAR sensor detecting iRFP720
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFU-GW-sMYH6-mNeon-TNNT2-mScarlet
Plasmid#170712Purposedual fluorescent reporter for MYH6 (short) and TNNT2 promotersDepositorInsertsmNeonGreen
mScarlet
UseLentiviralTagsExpressionMammalianMutationPromoterTNNT2 and aMHCAvailabilityAcademic Institutions and Nonprofits only -
pJRH051
Plasmid#171625Purpose2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreenDepositorInsertsspyCas9 sgRNA-BsmBI-Destination
mNeon
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1-a and U6AvailabilityAcademic Institutions and Nonprofits only -
pTub-alpha-EB3-NeonGreen
Plasmid#175263Purposelabelling of microtubule plus end and F-actin structuresDepositorInsertEB3 (MAPRE3 Human)
UseTagsmNeonGreenExpressionMammalianMutationNone (wt)PromoterpTub-alpha1AvailabilityAcademic Institutions and Nonprofits only -
pLR5-CAG-TetR_W43F-3xmNG
Plasmid#174880PurposePiggyBac transposon vector for expression of TetR(W43F)-3x-mNeonGreenDepositorInsertTetR(W43F)-3xmNeonGreen
UsePiggybac vectorTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEC3115 (pSpy0K6-P23_mKate2~*ssrA)
Plasmid#218509Purposeintegrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombinationDepositorInsertmKate2
UseTagsssrA degradation tagExpressionBacterialMutationDeletion of Plac_mrfp cassette, introduction of a…PromoterP23AvailabilityAcademic Institutions and Nonprofits only -
L40C-CRISPR.EFS.mNeon
Plasmid#69146PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), mNeon coexpression, EFS Promoter drivenDepositorInsertsSpCas9
Sp sgRNA scaffold
EFS
P2A-mNeonGreen
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 521 - 540 of 581 results