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pGEX SHP-2(N)-SH2

Plasmid

#46498

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHP-2(N) (PTPN11 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-104

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX SHP-2(NC)-SH2

Plasmid

#46499

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHP-2(NC) (PTPN11 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-222

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX SHP-2(C)-SH2

Plasmid

#46497

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHP-2(C) (PTPN11 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 101-222

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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ABHD2-3'UTR-Del-2

Plasmid

#190096

Purpose

examine the activity of ABHD2 3'UTR

Depositor

Bowen Liu

Article

Liu et al Commun Biol. 2022 Aug 13;5(1):816. doi:

Insert

ABHD2 3' UTR delete-2 (ABHD2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

deleted the base pairs 7122-7129 refer to NM_0070…

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-BI-hTFR1-2

Plasmid

#218797

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Article

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

AAV Rep-Cap BI-hTFR1-2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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161+80pCalb2/mutCREB(-2)-like

Plasmid

#66751

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp +80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

GCGC (-2) to TAGA

Promoter

Calb2

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-p35S-GFP(rc)2

Plasmid

#127521

Purpose

Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 2 attachment sites.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

EGFP reverse complement coding sequence flanked by attB/attP Integrase 2 attachment sites.

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pEF-GFP(rc)2

Plasmid

#127504

Purpose

Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 2 attachment sites.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

EGFP reverse complement coding sequence flanked by attB/attP Integrase 2 attachment sites.

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLIB_GST-nsp13 (SARS-CoV-2)

Plasmid

#169190

Purpose

Baculoviral transfer vector to express GST-nsp13 (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Zeng et al Biochem J. 2021 Jul 16;478(13):2405-2423

Insert

GST-nsp13 (ORF1ab SARS-CoV-2, Synthetic)

Use

Tags

GST

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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Beta-2-adrenergic receptor-mRFP

Plasmid

#67145

Purpose

Beta-2-adrenergic receptor tagged with mRFP on carboxyl terminus

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

Beta-2-adrenergic receptor-mRFP (ADRB2 Discosoma sp., Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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Beta-2-adrenergic receptor-YFP

Plasmid

#67146

Purpose

Beta-2-adrenergic receptor tagged with YFP on carboxyl terminus

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

Beta-2-adrenergic receptor-YFP (ADRB2 Aequorea victoria, Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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Beta-2-adrenergic receptor-CFP

Plasmid

#55794

Purpose

Beta-2-adrenergic receptor tagged with ECFP on carboxyl terminus

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

Beta-2-adrenergic receptor-ECFP (ADRB2 Aequorea victoria, Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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Beta-2-adrenergic receptor-mCerulean

Plasmid

#66957

Purpose

Beta-2-adrenergic receptor tagged with mCerulean on carboxyl terminus

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

Beta-2-adrenergic receptor-mCerulean (ADRB2 Aequorea victoria, Human)

Use

Tags

mCerulean

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 IL6

Plasmid

#67061

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

IL6 (IL6 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 SMAD2

Plasmid

#67095

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

SMAD2 (SMAD2 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCx43-7-miRFP670-2-N1

Plasmid

#197223

Purpose

Expresses the fusion protein of Cx43-7-miRFP670-2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

Cx43-7-miRFP670-2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pmiRFP670-2-Actin-C-18

Plasmid

#197218

Purpose

Expresses the fusion protein of miRFP670-2-Actin-C-18 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP670-2-Actin-C-18

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-PIG-miR-26a-2

Plasmid

#64230

Purpose

Expresses miR-26a-2 in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR-26a-2 (MIR26A2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pFB MBD2a-2-strep (pc1794)

Plasmid

#211715

Purpose

Expresses fl. MBD2 2xStrep tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2

Use

Tags

2xStrep

Expression

Insect

Mutation

Promoter

Polyhedrin Promoter

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mCherry-Nanobody (LaM-2)

Plasmid

#162276

Purpose

Expresses GST-tagged anti-mCherry Nanobody (LaM-2) in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

Insert

mCherry-Nanobody (LaM-2)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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