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pUCmini-RELA-PGK-tdTomato

Plasmid

#228054

Purpose

Homologous donor template for RELA knockout expressing tdTomato

Depositor

Yohei Sato

Article

Sato et al Clinical Immunology Communications, Volu

Insert

tdTomato

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-RELC-PGK-tdTomato

Plasmid

#228056

Purpose

Homologous donor template for RELC knockout expressing tdTomato

Depositor

Yohei Sato

Article

Sato et al Clinical Immunology Communications, Volu

Insert

tdTomato

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-7xHA-PEX6-Tomato

Plasmid

#183731

Purpose

Expression of human 7xHA- PEX6 fused to Tomato, and PEX6 3'UTR

Depositor

Jeffrey Gerst

Article

Haimovich et al bioRxiv 2024.11.06.622258

Insert

PEX6-Tomato-PEX63'UTR

Use

Lentiviral

Tags

7xHA

Expression

Mutation

Promoter

UbiC

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL3331 MTK234-TetO-AtoBpegRNA

Plasmid

#226668

Purpose

MTK234 part containing tet-inducible variant of the hU6 promoter driving an A to B pegRNA

Depositor

Chang Liu

Article

Loveless et al bioRxiv 2021.11.05.467507

Insert

hU6-TetO-AtoBpegRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

hU6-TetO

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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TALE-free DddAtox 1397C

Plasmid

#221941

Purpose

Expresses mitochondrially targeted TALE-free DddAtox 1397C-UGI in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-DddAtox 1397C-UGI-ATP5B 3'UTR

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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TALE-free DddAtox 1397N

Plasmid

#221942

Purpose

Expresses mitochondrially targeted TALE-free DddAtox 1397N-UGI in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-DddAtox 1397N-UGI-ATP5B 3'UTR

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pJT309 RapInducible1-ActivatorCloneSite-Citrine

Plasmid

#187321

Purpose

Rapamycin inducible genetic circuit with cloning site for activator domains

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-NFKB1-PGK-tdTomato

Plasmid

#204019

Purpose

Homologous donor template for NFKB1 knockout expressing tdTomato

Depositor

Yohei Sato

Article

Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

Insert

tdTomato

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-NFKB2-PGK-tdTomato

Plasmid

#204021

Purpose

Homologous donor template for NFKB2 knockout expressing tdTomato

Depositor

Yohei Sato

Article

Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

Insert

tdTomato

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-PRLSS-yATOM_SH2-KDEL

Plasmid

#209714

Purpose

Mammalian expression of y-ATOM biosensor for SH2 localized to endoplasmic reticulum

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

PRLSS-yATOM_SH2-KDEL

Use

Tags

PRLSS

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-MBP-yATOM_WDR5 (R80A)

Plasmid

#209713

Purpose

Mammalian expression of y-ATOM biosensor for WDR5 with binding null R80A mutation

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_WDR5 (R80A)

Use

Tags

MBP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCaf40-NFCVYRHAtoAAYYAAAF_X

Plasmid

#147978

Purpose

Insect Expression of DmCaf40-NFCVYRHAtoAAYAAAF

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-NFCVYRHAtoAAYAAAF (Rcd-1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCaf40-NFCVYAtoAAYYAF_W

Plasmid

#147829

Purpose

Insect Expression of DmCaf40-NFCVYAtoAAYYAF

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-NFCVYAtoAAYYAF (Rcd-1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCaf40-NFCVYAtoAAYYAF_W

Plasmid

#147838

Purpose

Insect Expression of DmCaf40-NFCVYAtoAAYYAF

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-NFCVYAtoAAYYAF (Rcd-1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGWT35S-AtOEP7(1-50aa)-mVenus

Plasmid

#194000

Purpose

Transient expression of AtOEP7-mVenus in plant cell (Chloroplast outer envelope membrane)

Depositor

Yutaka Kodama

Article

Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

Insert

mVenus

Use

Tags

AtOEP7 1–50aa

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGWT35S-AtOEP7(1-50aa)-VC155

Plasmid

#194046

Purpose

Transient expression of AtOEP7-VC155 (mVenus β-strands 8 to 11, amino acids 155−239) in plant cell (Chloroplast outer envelope membrane)

Depositor

Yutaka Kodama

Article

Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

Insert

VC155 (mVenus β-strands 8 to 11, amino acids 155−239)

Use

Tags

AtOEP7 1–50aa

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGWT35S-AtOEP7(1-50aa)-VN154

Plasmid

#194045

Purpose

Transient expression of AtOEP7-VN154 (mVenus β-strands 1 to 7, amino acids 1−154) in plant cell (Chloroplast outer envelope membrane)

Depositor

Yutaka Kodama

Article

Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

Insert

VN154 (mVenus β-strands 1 to 7, amino acids 1−154)

Use

Tags

AtOEP7 1–50aa

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCALNL-tdTomato-2A-mSyp::EGFP

Plasmid

#191213

Purpose

Expresses tdTomato protein and mouse Synaptophysin protein fused with EGFP in Cre expressing mammalian cells.

Depositor

Theofanis Karayannis

Article

Gesuita et al STAR Protoc. 2022 Nov 11;3(4):101837. do

Insert

tdTomato-T2A-mSyp::EGFP (Syp Mouse)

Use

Pcagen

Tags

mSyp::EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pXD050 dTomato GFP KIKO

Plasmid

#192155

Purpose

dTomato GFP KIKO

Depositor

Sidi Chen

Article

Dai et al Nat Methods. 2019 Mar;16(3):247-254. doi

Insert

dTomato GFP

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_CoordinatorMutant#2

Plasmid

#173986

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with Coordinator motif #2 mutated

Use

Luciferase

Tags

Expression

Mutation

Coordinator motif mutation #2

Promoter

SV40 promoter

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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