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Plasmid#186965PurposeExpress Arl6ip1 with C-terminus mEmeraldDepositorInsertARL6IP1 (ARL6IP1 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMV PromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG_TWIST1
Plasmid#215027PurposeMammalian expression of human TWIST1DepositorInserttwist family bHLH transcription factor 1 (TWIST1 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA pDEST40 Cas9-POLD3
Plasmid#183194PurposeExpression cloneDepositorInsertPOLD3
UseCRISPRTagsCas9WTExpressionMammalianMutationn/aPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + Needle
Plasmid#84868PurposeBaculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
Needle
UseBaculovirusTags6xHisExpressionInsectMutationPromoterPolyhedrinAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA pDEST40 Cas9-HMGB1
Plasmid#183195PurposeExpression cloneDepositorInsertHMGB1
UseCRISPRTagsCas9WTExpressionMammalianMutationn/aPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + Fla
Plasmid#84866PurposeBaculovirus Expression of LFn -Fla fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
Fla
UseBaculovirusTagsExpressionInsectMutationPromoterPolyhedrinAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCINeoFlag ALIX; I212D
Plasmid#89861PurposeExpresses full length Flag-tagged ALIX with mutation I212D in mammalian cells; internal ID WISP05-117DepositorInsertALIX (PDCD6IP Human)
UseTagsFlagExpressionMammalianMutationMutated isoleucine 212 to aspartic acidPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + FlaAAA
Plasmid#84867PurposeBaculovirus Expression of LFn-FlaAAA fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.DepositorInsertsLFn
FlaAAA
UseBaculovirusTags6xHisExpressionInsectMutation3 C-terminal leucines have been replaced by alani…PromoterPolyhedrinAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + PrgJ
Plasmid#84869PurposeBaculovirus Expression of LFn-PrgJ fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
PrgJ
UseBaculovirusTags6xHisExpressionInsectMutationPromoterPolyhedrinAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCINeoFlag ALIX; I318A
Plasmid#89867PurposeExpresses full length Flag-tagged ALIX with mutation I318A in mammalian cells; internal ID WISP11-403DepositorInsertALIX (PDCD6IP Human)
UseTagsFlagExpressionMammalianMutationMutated isoleucine 318 to alaninePromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-NOSTRIN
Plasmid#221309PurposeExpression of NOSTRIN (pLxIS) in mammalian cells by retroviral transductionDepositorInsertNOSTRIN (pLxIS) (Nostrin Mouse)
UseRetroviralTagsFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_V5-TWIST1
Plasmid#215030PurposeMammalian expression of human TWIST1DepositorInserttwist family bHLH transcription factor 1 (TWIST1 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Actin-mRFP-PAGFP
Plasmid#62382PurposeExpresses beta actin with both a PAGFP and mRFP tag.DepositorInsertbeta actin (ACTB Human)
UseTagsPAGFP and mRFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pFastBac-HS-NLRC4Δcard
Plasmid#177276PurposePurification of mouse NLRC4 (90-1024) from insect cellsDepositorInsertNLRC4 (Nlrc4 Mouse)
UseTagsHIS-SUMO-PreScissionExpressionInsectMutationI586T, N761S, M885V, K915R and F972L- please see …PromoterpolyhedrinAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV_FKBP-V5-PRRX1
Plasmid#215024PurposeAAV vector for knocking in an N-terminal tag (FKBP12F36V and V5) for human PRRX1DepositorInsertpaired related homeobox 1 (PRRX1 Human)
UseAAVTagsFKBP-V5ExpressionMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_V5-TWIST1_P139S
Plasmid#215040PurposeMammalian expression of human TWIST1 (with P139S mutation)DepositorInserttwist family bHLH transcription factor 1 (TWIST1 Human)
UseTagsV5ExpressionMammalianMutationP139SPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_V5-TWIST1_L138Y
Plasmid#215041PurposeMammalian expression of human TWIST1 (with L138Y mutation)DepositorInserttwist family bHLH transcription factor 1 (TWIST1 Human)
UseTagsV5ExpressionMammalianMutationL138YPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_V5-TWIST1_L138F
Plasmid#215042PurposeMammalian expression of human TWIST1 (with L138F mutation)DepositorInserttwist family bHLH transcription factor 1 (TWIST1 Human)
UseTagsV5ExpressionMammalianMutationL138FPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_MSX1-FKBP-mNeonGreen-V5
Plasmid#215025PurposeAAV vector for knocking in a C-terminal tag (FKBP12F36V, mNeonGreen, and V5) for human MSX1DepositorInsertmsh homeobox 1 (MSX1 Human)
UseAAVTagsFKBP-mNeonGreen-V5ExpressionMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only