Addgene

We narrowed to 2,043 results for: Xpa

Showing: 521 - 540 of 2043 results

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pBT13-splitD

Plasmid

#122597

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBT100.106b

Plasmid

#122601

Purpose

clone APOBEC1 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

APOBEC1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pFP.R267

Plasmid

#138179

Purpose

Expresses the split mCherry reporter interrupted by the gp41-1 intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(SGY).gp41-1.(SSS)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pFP.R268

Plasmid

#138180

Purpose

Expresses the split mCherry reporter interrupted by the gp41-8 intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(LNR).gp41-8.(SAV)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pFP.R270

Plasmid

#138219

Purpose

Expresses the split mCherry reporter interrupted by the IMPDH-1 intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(GGG).IMPDH-1.(SIC)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBT372

Plasmid

#125613

Purpose

Expresses evoCDA1-BE4max-NG in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoCDA1-BE4max-NG

Use

Tags

Expression

Mammalian

Mutation

F23S A123V I195F

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-B-GECO1

Plasmid

#32448

Purpose

Mammalian expression of blue fluorescent genetically encoded Ca2+ indicator for optical imaging

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 8.

Insert

B-GECO1.0

Use

Tags

Expression

Mammalian

Mutation

GCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…

Promoter

CMV

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

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pBT277

Plasmid

#122608

Purpose

express evoCDA1-BE4max in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoCDA1-BE4max

Use

Tags

Expression

Mammalian

Mutation

F23S A123V I195F

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBT371

Plasmid

#125612

Purpose

Expresses CDA1-BE4max-NG in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

CDA1-BE4max-NG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-StagRFP-AktAR

Plasmid

#173020

Purpose

Yellow-red FRET-based Akt/PKB activity reporter for multiplexed imaging applications.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

Venus-StagRFP-AktAR

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-RLuc

Plasmid

#188312

Purpose

Expression of Renilla luciferase via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

Renilla luciferase

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Mutation

Promoter

T7Max

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)::NL

Plasmid

#141289

Purpose

NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Insert

NLUC

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Engineered for high stability (t1/2 = 11.5 days a…

Promoter

promoter for bacteriophage T7 RNA polymerase

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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ABE8r-R153del

Plasmid

#208296

Purpose

Expresses ABE8r in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r-R153del-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCpf1b

Plasmid

#122187

Purpose

Bacterial genome editing plasmid with kanamycin resistance marker and using sacB as a counter-selection marker

Depositor

Cheng-Cai Zhang

Article

Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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ABE8r-V106W

Plasmid

#208295

Purpose

Expresses ABE8r in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r-V106W-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106W, …

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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PE-P3-rice

Plasmid

#185612

Purpose

For plant prime editing in rice plants

Depositor

Jinxiao Yang

Article

Xu et al Nat Plants. 2022 Jan;8(1):45-52. doi: 10

Insert

3XFLAG-4XNLS-M-MLV-nCas9(H840A)-4XNLS-P2A-Hyg

Use

CRISPR

Tags

Expression

Mutation

Promoter

ZmUbi

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCpf1b-sp

Plasmid

#122188

Purpose

Bacterial genome editing plasmid with spectinomycin resistance marker and using sacB as a counter-selection marker

Depositor

Cheng-Cai Zhang

Article

Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pBT120b

Plasmid

#122604

Purpose

BE PACE selection, express gIII, luxAB and degron-GTCCA guide RNA

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

gIII, luxAB, guide RNA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBT44c

Plasmid

#122600

Purpose

BE PACE selection, express C-intein-Cas9-ugi fusion

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

intein-dCas9-ugi fusion

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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p416 25Q GPD

Plasmid

#1177

Depositor

Susan Lindquist

Article

Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

Insert

htt 25Q (HTT Human)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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