Skip to main content
Addgene
Showing: 521 - 540 of 12893 results

  3. p5E-Runx1+23

    Plasmid
    #69602
    Purpose
    Runx1 +23 kb intronic enhancer from mouse and beta-globin minimal promoter in a 5' entry vector for gateway cloning
    Depositor
    Owen Tamplin, Leonard Zon
    Article
    Tamplin et al Cell. 2015 Jan 15;160(1-2):241-52. doi:
    Inserts
    Runx1 +23 intronic enhancer (Runx1 Mouse)
    Mouse beta-globin minimal promoter
    Use
    5' gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    C57/BL6 mouse Runx1 +23 intronic enhancer and C57…
    Available since
    Sept. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    69602-plasmid-map-sequence-id-190603

    Enlarge

  9. p1.1-mCherry

    Plasmid
    #162772
    Purpose
    Fluorescent reporter for CHO expression studies
    Depositor
    Ivan Vorobiev
    Article
    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2
    Insert
    red fluorescent protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    162772-plasmid-map-sequence-id-339102

    Enlarge

  10. p1.2-GS-eGFP

    Plasmid
    #162771
    Purpose
    Fluorescent reporter for CHO expression studies
    Depositor
    Ivan Vorobiev
    Article
    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2
    Insert
    enhanced green fluorescent protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    162771-plasmid-map-sequence-id-339094

    Enlarge

  11. COVID-SARS2 NSP14/10

    Plasmid
    #159613
    Purpose
    Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10
    Depositor
    Opher Gileadi
    Article
    Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650
    Inserts
    Covid-SARS2 Nsp14 (ORF1ab Synthetic, COVID-SARS2)
    Covid-SARS2 Nsp10
    Use
    Tags
    His6, TEV cleavage site and none
    Expression
    Bacterial
    Mutation
    Codon-optimized for E. coli expression
    Promoter
    (Bicistronic) and T7 - LacO
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    159613-plasmid-map-sequence-id-311056

    Enlarge

  12. YFP(1-158)-beta-1 in pcDNAI/Amp

    Plasmid
    #54468
    Purpose
    An amino-terminal YFP fragment was fused to Gbeta-1. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8
    Insert
    YFP (1-158)/beta-1 (GNB1 Aequorea victoria, Human)
    Use
    Tags
    YFP(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    GBeta-1 was amplified via PCR, which removed an i…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    54468-plasmid-map-sequence-id-112586

    Enlarge

  13. pEH_AAV_TERT_prom_-124C>T_C7C_1.8kb

    Plasmid
    #183080
    Purpose
    rAAV transfer plasmid with ITRs flanking: (1) TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.
    Depositor
    Aviv Regev
    Article
    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.
    Insert
    TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    Use
    AAV; Homologous recombination donor template
    Tags
    Expression
    Mutation
    -124C>T promoter, Exon 1 C7C (C21>T21) sile…
    Promoter
    None for primary insert (Though partial TERT prom…
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    183080-plasmid-map-sequence-id-363419

    Enlarge

  14. pEH_AAV_TERT_prom_-146C>T_C7C_1.8kb

    Plasmid
    #183081
    Purpose
    rAAV transfer plasmid with ITRs flanking: (1) TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.
    Depositor
    Aviv Regev
    Article
    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.
    Insert
    TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    Use
    AAV; Homologous recombination donor template
    Tags
    Expression
    Mutation
    -146C>T promoter, Exon 1 C7C (C21>T21) sile…
    Promoter
    None for primary insert (Though partial TERT prom…
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    183081-plasmid-map-sequence-id-363415

    Enlarge

  15. pEH_AAV_TERT_prom_C7C_1.8kb

    Plasmid
    #183079
    Purpose
    rAAV transfer plasmid with ITRs flanking: (1) TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.
    Depositor
    Aviv Regev
    Article
    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.
    Insert
    TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    Use
    AAV; Homologous recombination donor template
    Tags
    Expression
    Mutation
    Wildtype promoter, Exon 1 C7C (C21>T21) silent…
    Promoter
    None for primary insert (Though partial TERT prom…
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    183079-plasmid-map-sequence-id-363414

    Enlarge

  16. pAcGFP-IVT

    Plasmid
    #205145
    Purpose
    IVT template of AcGFP with MCS in frame and polyA
    Depositor
    Igor Ulitsky
    Insert
    AcGFP1
    Use
    In-vitro transcription
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    205145-plasmid-map-sequence-id-404881

    Enlarge

  17. pMDW139

    Plasmid
    #227336
    Purpose
    Template for in vitro Pol II transcription
    Depositor
    Michelle Wang
    Insert
    Gap DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Plac
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    227336-plasmid-map-sequence-id-451681

    Enlarge

  18. pAcGFP-stop-IVT

    Plasmid
    #205146
    Purpose
    IVT template of AcGFP stop codon between the ORF and MCS and polyA
    Depositor
    Igor Ulitsky
    Insert
    AcGFP1
    Use
    In-vitro transcription
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    205146-plasmid-map-sequence-id-404879

    Enlarge

  19. pFFLuc-IVT

    Plasmid
    #205147
    Purpose
    IVT template of FireFly luciferase stop codon between the ORF and MCS and polyA
    Depositor
    Igor Ulitsky
    Insert
    FireFly luciferase
    Use
    Luciferase; In-vitro transcription
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    205147-plasmid-map-sequence-id-404882

    Enlarge

  20. pRLuc-IVT

    Plasmid
    #205148
    Purpose
    IVT template of Renilla luciferase stop codon between the ORF and MCS and polyA
    Depositor
    Igor Ulitsky
    Insert
    Renilla Luciferase
    Use
    Luciferase; In-vitro transcription
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    205148-plasmid-map-sequence-id-404883

    Enlarge

Showing: 521 - 540 of 12893 results