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pKDsgRNA-rpsL

Plasmid

#89953

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rpsL.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

rpsL gRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-ldhA

Plasmid

#102288

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting ldhA.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

ldhA gRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-malG

Plasmid

#89951

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting malG.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

malG gRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-hisG

Plasmid

#89954

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting hisG.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

hisG gRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-trmI

Plasmid

#89955

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting trmI.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

trmI gRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-xylA

Plasmid

#89964

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting xylA.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

xylA gRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-lpoB

Plasmid

#89959

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting lpoB.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

lpoB gRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDsgRNA-fsaA

Plasmid

#102286

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting fsaA.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

fsaA gRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-adhE

Plasmid

#102290

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting adhE.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

adhE gRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-ompT

Plasmid

#102291

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting ompT.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

ompT gRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDsgRNA-srlA

Plasmid

#89965

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting srlA.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

srlA gRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDsgRNA-rbsK

Plasmid

#89963

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rbsK.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

rbsK gRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDsgRNA-yhhT

Plasmid

#89958

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting yhhT.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

yhhT gRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDsgRNA-rssA

Plasmid

#89957

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

rssA gRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-torZ

Plasmid

#89956

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting torZ.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

torZ gRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDsgRNA-arsB

Plasmid

#89952

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting arsB.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

arsB gRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pYY_S1_400

Plasmid

#170470

Purpose

To amplify transcription template containing LacI operators

Depositor

Laura Finzi

Article

Kovari et al Methods Mol Biol. 2018;1665:317-340. doi

Insert

T7A1, Os, O1, lambda t1, in lacZ fragment

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7A1

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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LMB

Plasmid

#12502

Depositor

David Baker

Article

Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60.

Insert

LMB

Use

Tags

6xHis

Expression

Bacterial

Mutation

RosettaDesign was used to design sequences. Align…

Promoter

Available since

March 13, 2007

Availability

Academic Institutions and Nonprofits only
pMM441

Plasmid

#61271

Purpose

Also referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.

Depositor

Timothy Lu

Article

Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

Insert

Cas9, tracrRNA, crRNA

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCasKP-apr

Plasmid

#117231

Purpose

Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge