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  11. pSF3-Flag-Cbir-Cdc25C S216A_Myc-Nbir-14-3-3e

    Plasmid
    #90011
    Purpose
    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C S216A and N-terminally tagged NBir[2-256]-14-3-3e
    Depositor
    Julien Béthune
    Article
    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.
    Inserts
    CBirA*-Cdc25C S216A
    NBirA*-14-3-3e
    Use
    Retroviral; Flp/frt
    Tags
    FLAG and Myc
    Expression
    Mammalian
    Mutation
    Ser 216 to Ala
    Promoter
    CMV
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    90011-plasmid-map-sequence-id-201243

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  13. pEGFP-C3-PLK4-K41M-S285A-T289A-3xFLAG

    Plasmid
    #69842
    Purpose
    This plasmid encodes kinase dead PLK4 isoform 1 carrying K41M and S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag
    Depositor
    Michel Bornens
    Article
    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.
    Insert
    PLK4 (PLK4 Human)
    Use
    Tags
    3xFLAG tag and EGFP
    Expression
    Mammalian
    Mutation
    K41M-S285A-T289A
    Promoter
    CMV
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    69842-plasmid-map-sequence-id-123881

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  14. pMIG-AVITEV- hNFATc1/aA

    Plasmid
    #74057
    Purpose
    retroviral expression plasmid for human NFATc1/aA with N-terminal BirA-biotinylation signal and TEV celavage site
    Depositor
    Ria Baumgrass
    Article
    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.
    Insert
    human NFATc1, isoform alpha-A (NFATC1 Human)
    Use
    Retroviral
    Tags
    AVI-TEV
    Expression
    Mammalian
    Mutation
    mutation G1157A [R235Q] and the silent mutation C…
    Promoter
    pMSCV-LTRs
    Available since
    June 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    74057-plasmid-map-sequence-id-142263

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  15. pMIG-hNFATc2/C(RIT)-FLAG-AVITEV

    Plasmid
    #74053
    Purpose
    retroviral expression plasmid for human NFATc2/C (with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage site
    Depositor
    Ria Baumgrass
    Article
    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.
    Insert
    human NFATc2, isoform C (NFATC2 Human)
    Use
    Retroviral
    Tags
    AVI-TEV and FLAG
    Expression
    Mammalian
    Mutation
    silent mutation A1723C in the sequence of NM_1730…
    Promoter
    pMSCV-LTRs
    Available since
    June 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    74053-plasmid-map-sequence-id-142273

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  16. pMIG-hNFATc2/C(1-686)AVITEV

    Plasmid
    #74054
    Purpose
    retroviral expression plasmid for human NFATc2/C (without C-terminal region) with C-terminal BirA-biotinylation signal and TEV celavage site
    Depositor
    Ria Baumgrass
    Article
    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.
    Insert
    human NFATc2, isoform C (NFATC2 Human, AS 1-686 (N terminus deleted))
    Use
    Retroviral
    Tags
    AVI-TEV
    Expression
    Mammalian
    Mutation
    silent mutation A1723C in the sequence of NM_1730…
    Promoter
    pMSCV-LTRs
    Available since
    June 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    74054-plasmid-map-sequence-id-142271

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  17. pMIG-hNFATc2/C(1-686, RIT)FLAG-AVITEV

    Plasmid
    #74055
    Purpose
    retroviral expression plasmid for human NFATc2/C(1-686, RIT) (without C-terminal region, with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage site
    Depositor
    Ria Baumgrass
    Article
    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.
    Insert
    human NFATc2, isoform C (NFATC2 Human, AS 1-686 (N terminus deleted))
    Use
    Retroviral
    Tags
    AVI-TEV and FLAG
    Expression
    Mammalian
    Mutation
    silent mutation A1723C in the sequence of NM_1730…
    Promoter
    Available since
    June 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    74055-plasmid-map-sequence-id-142272

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  18. pFA6a-link-mKO1-SpHis5

    Plasmid
    #44642
    Purpose
    Yeast tagging vector to create gene fusion with mKO1 with SpHis5 selection
    Depositor
    Kurt Thorn
    Insert
    mKO1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only
    44642-plasmid-map-sequence-id-61105

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  19. pFA6a-link-mKO1-CaUra3

    Plasmid
    #44638
    Purpose
    Yeast tagging vector to create gene fusion with mKO1 with CaUra3 selection
    Depositor
    Kurt Thorn
    Insert
    mKO1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only
    44638-plasmid-map-sequence-id-61102

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  20. pBAD-FimH-9xHis

    Plasmid
    #97305
    Purpose
    E.coli UTI89 FimH signal peptide and lectin domain
    Depositor
    Per Svenningsen
    Insert
    Type 1 fimbiral adhesin FimH
    Use
    Tags
    9xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    97305-plasmid-map-sequence-id-182575

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Showing: 521 - 540 of 562 results