-
Plasmid#137680PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHM086Env
Plasmid#137666PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHM032Env
Plasmid#137683PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcNGsC-MutY-pKK223
Plasmid#131376PurposeLow level expression of E.coli G.stearothermophillus MutY chimera proteinDepositorInsertEcNGsC MutY chimera
UseTagsExpressionBacterialMutationFusion of two MutY genes. Residues 1-225 are from…PromotertacAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NCKIPSD-1/1
Plasmid#91376PurposeProtein expression and purification of human SH3 domain construct NCKIPSD-1/1DepositorInsertNCKIPSD-1/1 (NCKIPSD Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Gm129-Flag
Plasmid#53766Purposebaculovirus expression of Gm129DepositorInsertGm129 (Ciart Mouse)
UseTagsFLAGExpressionInsectMutationPromoterPolyhedrinAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSMP-MBD4_3
Plasmid#36376DepositorInsertMBD4 (MBD4 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH398-SUP45-G54D
Plasmid#29376DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
UseTagsExpressionYeastMutationG54D mutant gene including genomic sequence 1432 …PromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV/D377Y-mPCSK9
Plasmid#58376PurposeExpresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosisDepositorInsertproprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)
UseAAVTagsExpressionMammalianMutationD377YPromoterHCRApoE/hAATAvailable sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-P112H
Plasmid#133760PurposeLentiviral expression of human TDP-43 P112H disease mutant in mammalian cells.DepositorInsertTDP-43 (TARDBP Human)
UseLentiviralTagsExpressionMammalianMutationChanged proline 112 to histidine.PromoterCMVAvailable sinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNS20-SpCas9-SNAP
Plasmid#113717PurposeBacterial vector for expression of Snap-tagged Streptococcus pyogenes Cas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-Q331K
Plasmid#133761PurposeLentiviral expression of human TDP-43 Q331K disease mutant in mammalian cells.DepositorInsertTDP-43 (TARDBP Human)
UseLentiviralTagsExpressionMammalianMutationChanged glutamine 331 to lysine.PromoterCMVAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
proISG15 (2-165)
Plasmid#110762PurposeBacterial expression of proISG15DepositorInsertISG15 (ISG15 Human)
UseTagsN-His6-3CExpressionBacterialMutationPromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBR_attB(bxb)_ccdB_lox
Plasmid#183763PurposeBxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)DepositorInsertccdB
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNS38-SadCas9-SNAP
Plasmid#113718PurposeBacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9DepositorInsertCas9
UseCRISPRTagsHA-2xNLS-SNAP-NLS and His6-MBP-TEVExpressionBacterialMutationD10A, N580APromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX462-hPRKAA2-gRNA_A
Plasmid#74376PurposegRNA_A to knockout human AMPK alpha 2 using Cas9n.DepositorInserthuman AMPK alpha 2 exon1 gRNA (PRKAA2 Human)
UseCRISPRTagsExpressionMutationPromoterU6Available sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-4580NES
Plasmid#138376PurposeBooster-JNK, a Red-Shifted Genetically Encoded FRET Biosensor for JNK activity.DepositorInsertBooster-JNK
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Bxb1_AJMA
Plasmid#183760PurposeBxb1-specific donor plasmid for integrating transcription units for expressing ASAP2f (2 copies), jRCaMP1b and membrane-localised miRFP703DepositorInsertsASAP2f
jRCaMP1b
miRFP703
ASAP2f
UseTagsLck and NESExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
plenti-UbcP-Myc-CRBN-pGK-HYG
Plasmid#107376PurposeLentiviral expression of myc-CRBNDepositorInsertcereblon (CRBN Human)
UseLentiviralTagsMycExpressionMammalianMutationPromoterUbcPAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNS19-LV-mutRFP-2A-GFP
Plasmid#113716PurposeLentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)DepositorInsertmutRFP-2A-GFP-IRES-Puro
UseLentiviral; Reporter systemTagsExpressionMammalianMutationY64L (mutRFP)PromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only