-
Plasmid#201400PurposeKnockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertCOL1A1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAB7A-IRES-Puromycin
Plasmid#133027PurposeExpresses EGFP-tagged late endosome markerDepositorInsertRAB7A (RAB7A Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterHuman elongation factor 1 alphaAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-TA Bik-IRES-Puromycin
Plasmid#133026PurposeExpresses EGFP-tagged tail anchor sequence of BikDepositorInsertBcl-2-interacting killer (BIK Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 1-110PromoterHuman elongation factor 1 alphaAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP.mIKKα
Plasmid#74413PurposeExpresses mouse eGFP-IKKα in mammalian cellsDepositorInsertmouse IKKα (Chuk Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRRL.PPT.SFFV.HA.RUNX1C.IRES.eGFP
Plasmid#181980PurposeHuman RUNX1C gene overexpressionDepositorInsertRUNX1C (human) (RUNX1 Human)
UseLentiviralTagsHA (Human influenza hemagglutinin)ExpressionMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.TRE.HA.RUNX1A.IRES.dTomato.PGK.sfGFP.P2A.Tet3G
Plasmid#181977PurposeHuman RUNX1A gene overexpressionDepositorInsertRUNX1A (human) (RUNX1 Human)
UseLentiviralTagsHA (Human influenza hemagglutinin)ExpressionMutationPromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRRL.PPT.SFFV.HA.RUNX1A.IRES.eGFP
Plasmid#181979PurposeHuman RUNX1A gene overexpressionDepositorInsertRUNX1A (human) (RUNX1 Human)
UseLentiviralTagsHA (Human influenza hemagglutinin)ExpressionMutationPromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.TRE.HA.RUNX1C.IRES.dTomato.PGK.sfGFP.P2A.Tet3G
Plasmid#181978PurposeHuman RUNX1C gene overexpressionDepositorInsertRUNX1C (human) (RUNX1 Human)
UseLentiviralTagsHA (Human influenza hemagglutinin)ExpressionMutationPromoterAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcryGFP-acta1-dmdGFP
Plasmid#172530PurposealphaA-crystallin promoter (cry) drives GFP expression in the lens. The acta1 muscle-specific promoter drives expression of GFP-tagged zebrafish dmd.DepositorInsertdmd (dmd Zebrafish)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
T_antiEGFPsynsrRNA_UA
Plasmid#185974PurposeExpresses antiEGFPsynsrRNA from PlTetO-1 promoter in E.coli Dh5alpha cell. For experiment use Dh5alphaz1.DepositorInsertPlTetO-1 AntiEGFPsynsrRNA
UseSynthetic BiologyTagsExpressionMutationPromoterPlTetO-1Available sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
L_EGFP_AC
Plasmid#185971PurposeExpresses EGFP from PlLaco-1 promoter in E.coli Dh5alpha cell. For experiment use Dh5alphaz1DepositorInsertPl-LacO1-Egfp
UseSynthetic BiologyTagsExpressionMutationPromoterPlLacO-1Available sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-APP-mGFP
Plasmid#196704PurposeExpresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cellsDepositorInsertAmyloid Precursor Protein 695 isoform (APP Human)
UseTagsmCherry and mEGFPExpressionMammalianMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
mBFP-APP-mGFP
Plasmid#196694PurposeExpresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cellsDepositorInsertAmyloid Precursor Protein 695 isoform (APP Human)
UseTagsmEGFP and mtagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
APP_pcDNA6.2/EmGFP-Bsd
Plasmid#176954PurposeMammalian expression vector encoding APP and EmGFP-BsdDepositorInsertAPP (APP Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
DNMT3ACD-CRY2-EGFP
Plasmid#82556PurposeEncodes DMNT3 under LITE2.0 systemDepositorUseAAVTags2A, NLS(alpha-imp), and NLS-VP64ExpressionMammalianMutationCRY2PHR (N-terminal photolyase homology region do…PromoterEF-1alphaAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-Cstem-Tac(5A)
Plasmid#162497PurposeExpresses GFP tagged Tac mutant in mammalian cells. The five O-glycosylation sites in the stem region are mutated, and the stem region of CD8a is inserted between GFP and Tac(5A).DepositorUseTagsGFPExpressionMammalianMutationThe five threonine residues are mutated into alan…PromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP Rab11a WT pcDNA3.1 Zeo (+)
Plasmid#206079PurposeRab11a belongs to the Rab family of small GTPases, Rab11 proteins are particularly involved in trafficking through recycling endosomesDepositorInsertRAB11A (RAB11A Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
FR_GFP
Plasmid#31302DepositorInsertgreen fluorescent protein
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EphrinB3-Flag(A227Y)
Plasmid#155013PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 A227Y variant from pcDNA3.1DepositorInsertEphrinB3 (EPHB3 Human)
UseTagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationA227YPromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GFP-IRES-hSNCA
Plasmid#185715PurposeAAV expression of GFP and human α-Synuclein from hSyn promoterDepositorInsertsynuclein, alpha (SNCA Human)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only