Addgene

We narrowed to 6,831 results for: 11

Showing: 1301 - 1350 of 6831 results

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phU6 ST

Plasmid

#188712

Purpose

sgRNA plasmid encoding hU6 promoter driving an anti-safe targeting sgRNA

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

Safe Targeting sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pmU6 ST

Plasmid

#188713

Purpose

sgRNA plasmid encoding mU6 promoter driving an anti-safe targeting sgRNA

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

Safe Targeting sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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p7SK ST

Plasmid

#188714

Purpose

sgRNA plasmid encoding 7SK promoter driving an anti-safe targeting sgRNA

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

Safe Targeting sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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phH1 ST

Plasmid

#188715

Purpose

sgRNA plasmid encoding hH1 promoter driving an anti-safe targeting sgRNA

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

Safe Targeting sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAGT707

Plasmid

#51835

Purpose

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Tomato Mosaic virus 5'UTR+ CCAT

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

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pAND_yfp

Plasmid

#60592

Purpose

used with pSpec to generate an AND gate without memory

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

AND_yfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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V5-W279A-short-UBASH3A

Plasmid

#192086

Purpose

Mammalian expression of human W279A short-form UBASH3A with V5 tag

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Use

Tags

V5

Expression

Mammalian

Mutation

W279A

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMiniWhiteA2

Plasmid

#165873

Purpose

Basic transgenesis Mini-white physical eye marker cassette in Alpha2, lacks attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Eye Marker Cassette

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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D588 lys2::HIS3 Disruptor Converter

Plasmid

#51675

Purpose

Yeast marker swap plasmid for converting LYS2 to HIS3

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

HIS3 (HIS3 Budding Yeast)

Use

Yeast marker swap

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pEnt L1L3 tTA-2

Plasmid

#24414

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Type

Empty backbone

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH87611

Plasmid

#50284

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Promoter and 5'UTR Ls (S.tuberosum)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH50581

Plasmid

#50256

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, act2 (AT3G18780, Arabidopsis thaliana)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pDH003

Plasmid

#157640

Purpose

sMHT-113 fused to SYNZIP17/18

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)-SYNZIP17
SYNZIP18-sMHT(114-230)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEnt R3L2-MCS

Plasmid

#26799

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

multiple cloning sequence

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Xt-VSP

Plasmid

#51887

Purpose

expression of Xt-VSP in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP

Use

Xenopus oocyte expression

Tags

Expression

Mammalian

Mutation

see comments*

Promoter

CMV

Available since

April 3, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Xl-VSP1

Plasmid

#51880

Purpose

expression of Xl-VSP1 in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP1 (tpte2 Frog)

Use

Xenopus oocyte expression

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH41402

Plasmid

#50285

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-MmUbc7mut/2777

Plasmid

#11489

Depositor

Allan Weissman

Article

Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

Insert

MmUbc7 (Ube2g2 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

cys 889 mutated to ser

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pICSL30006

Plasmid

#50303

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS no stop codon, GFP (A. victoria)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-KG-MmUbc7/2783

Plasmid

#11490

Depositor

Allan Weissman

Article

Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

Insert

MmUbc7 (Ube2g2 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

n/a

Promoter

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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pHu6-gRNA-NT1

Plasmid

#81203

Purpose

hU6 expression of gRNA NT1

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA NT1

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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p5xUAS

Plasmid

#165891

Purpose

Synthetic 5xUAS GAL4 driver response element in enhancer grammar, requires minimal promoter for assembly

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

5xUAS

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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p5xQUAS

Plasmid

#165818

Purpose

Synthetic 5xQUAS QF driver response element in enhancer grammar, requires minimal promoter for assembly

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

5xQUAS

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH44199

Plasmid

#50286

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Potato Virus X)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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EGFP-mOrange pHis-//

Plasmid

#53302

Purpose

Expresses His-tagged EGFP-mOrange fusion protein.

Depositor

John Lis

Article

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Insert

EGFP

Use

Tags

His and mOrange

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmGe1-V5His6_A

Plasmid

#145892

Purpose

Insect Expression of DmGe1

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

DmGe1 (Ge-1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH45125

Plasmid

#50261

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, LHB1B1 (AT2g34430, A. thaliana)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pVP16 AD

Plasmid

#165822

Purpose

VP16 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

VP16 AD

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH49477

Plasmid

#50324

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, DsRed variant of RFP (Discosoma sp.)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Xl-VSP2

Plasmid

#51885

Purpose

expression of Xl-VSP2 in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP2

Use

Xenopus oocyte expression

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH41551

Plasmid

#50264

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, Ls (Solanum tuberosum)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pUPD2

Plasmid

#165856

Purpose

Universal Part domesticator vector v2 used for integration (domestication) of new DNA elements in the GB2.0 cloning system uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAdh 3'UTR Short

Plasmid

#165800

Purpose

Drosophila HSP70B 3'UTR sequence

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Hsp70 3'UTR

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDmHsp70b

Plasmid

#165785

Purpose

Drosophila HSP70B promoter fragment, shows basal expression that can be further enhanced via heatshock

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Hsp70b

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p3xP3-Hsp70b

Plasmid

#165773

Purpose

HSP70B Promoter fused to synthetic 3xP3 eye driver

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

3x3-Hsp70b

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
5xGMR

Plasmid

#165775

Purpose

5xGMR syntethic eye driver enhancer, requires minimal promoter element

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

5xGMR

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMiniwhiteA1

Plasmid

#165872

Purpose

Basic transgenesis Mini-white physical eye marker cassette in Alpha1, lacks attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Eye Marker Cassette

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZ414-F13

Plasmid

#23056

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pEnt L1L3-MCS

Plasmid

#26798

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

multiple cloning sequence

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

May 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH41388

Plasmid

#50253

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (0.4 kb), 35s (Cauliflower Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pHSP70B-CP6-hph

Plasmid

#165879

Purpose

Basic Hygromycin resistance cassette in Alpha1, lacks physical marker or attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJD427

Plasmid

#154371

Purpose

Expresses FKBP12-XB and SspBcore-FRB from constitutive promoters

Depositor

Joseph Davis

Article

Davis et al ACS Chem Biol. 2011 Nov 18;6(11):1205-13

Insert

proB-RBS(B0032)-SspBcore-FRB-terminator(B0011)-proC-RBS(B0032)-FKBP12-sspBXB-terminator(B0011)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-MAVS-M1A-invitro(KaganE12)

Plasmid

#52016

Purpose

Used for in vitro expression of the human MAVS CDS containing a point mutation M1A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M1A

Use

Tags

Expression

Mammalian

Mutation

changed Met 1 to Ala

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-G67M-invitro(KaganD100)

Plasmid

#52014

Purpose

Used for in vitro expression of the human MAVS CDS containing a point mutation G67M

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Use

Tags

Expression

Mammalian

Mutation

Changed Gly 67 to Met

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-L62M-invitro(KaganD98)

Plasmid

#52013

Purpose

Used for in vitro expression of the human MAVS CDS containing a point mutation L62M

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Use

Tags

Expression

Mammalian

Mutation

Changed Leu 62 to Met

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-E80M-invitro(KaganD99)

Plasmid

#52015

Purpose

Used for in vitro expression of the human MAVS CDS containing a point mutation E80M

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Use

Tags

Expression

Mammalian

Mutation

Changed Glu 80 to Met

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-M142A-invitro(E13)

Plasmid

#52017

Purpose

Used for in vitro expression of the human MAVS CDS containing a point mutation M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M142A

Use

Tags

Expression

Mammalian

Mutation

changed Met 142 to Ala

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pICH71342

Plasmid

#50283

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter and 5'UTR, H4 (S. lycopersicum)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpec

Plasmid

#60593

Purpose

used with pAND_YFP to generate an AND gate without memory

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Spec

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pRPLC

Plasmid

#122261

Purpose

pBRBAD-rbsD-RFP-B0015- B0015-LasR-rbs34-J23108-pBRlasI-rbsD-RFP- B0015

Depositor

Chueh Loo Poh

Article

Jayaraman et al ACS Synth Biol. 2018 Nov 16;7(11):2627-2

Insert

pBRBAD, pBRlasI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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