Skip to main content
Addgene
Showing: 521 - 540 of 150392 results

  3. pAAV/hSyn-SCLM

    Plasmid
    #216760
    Purpose
    AAV2 transfer vector with the human synapsin I promoter for neuronal expression of SuperClomeleon
    Depositor
    George Augustine
    Insert
    SuperClomeleon
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    S30R and 11 AA deletion in the Cerulean CFP moiet…
    Promoter
    hSyn (human synapsin I)
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216760-plasmid-map-sequence-id-429492

    Enlarge

  4. pGR618

    Plasmid
    #213786
    Purpose
    Integrates at the CAN1 locus the BB-Tv TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette
    Depositor
    Chang Liu
    Insert
    BB-Tv TP-DNAP1
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213786-plasmid-map-sequence-id-424624

    Enlarge

  5. pET23a-H6-TEV-cAbl

    Plasmid
    #214234
    Purpose
    Bacterial expression of the kinase domain of cAbl with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings
    Depositor
    Neel Shah
    Article
    Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1
    Insert
    ABL1 Kinase domain (ABL1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214234-plasmid-map-sequence-id-422815

    Enlarge

  6. LLP617_αGCN4-mCherry

    Plasmid
    #211785
    Purpose
    SunTag counterpart binding domain, aGCN4, fused to catalytically inert mCherry control, with GFP selection
    Depositor
    Ryan Lister
    Article
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Insert
    SnoopCatcher-DNMT3A
    Use
    Tags
    2xOLLAS
    Expression
    Mammalian
    Mutation
    Last 300 bp codon-optimised (CO) to detect DNMT3A…
    Promoter
    pEF1a and pSV40
    Available since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    211785-plasmid-map-sequence-id-417372

    Enlarge

  7. piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)

    Plasmid
    #209086
    Purpose
    Ngn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expression
    Depositor
    Vikram Khurana
    Article
    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.
    Inserts
    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    BirA
    Use
    Tags
    AviTag
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    TRE4G and hSyn
    Available since
    Dec. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    209086-plasmid-map-sequence-id-417159

    Enlarge

  8. pCEP4-myc-EFNB2 (D62Q-Q130L-V167L)

    Plasmid
    #200977
    Purpose
    Mammalian expression plasmid for myc-tagged EFNB2 (D62Q-Q130L-V167L specificity mutant)
    Depositor
    Erik Procko
    Article
    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do
    Insert
    EFNB2 (EFNB2 Human)
    Use
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Mutation
    D62Q-Q130L-V167L (increase specificity towards he…
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    200977-plasmid-map-sequence-id-397620

    Enlarge

  12. pLNHA-C1-HseIF4E_K

    Plasmid
    #146732
    Purpose
    Mammalian Expression of HseIF4E
    Depositor
    Elisa Izaurralde
    Insert
    HseIF4E (EIF4E Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146732-plasmid-map-sequence-id-289178

    Enlarge

  13. TFORF0664

    Plasmid
    #141655
    Purpose
    Lentiviral vector for overexpressing the NKX1-2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NKX1-2 (NKX1-2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141655-plasmid-map-sequence-id-427303

    Enlarge

  14. pCMV-SUN1 GFP

    Plasmid
    #187682
    Purpose
    In vivo visualization of SUN1
    Depositor
    Mary Beckerle
    Insert
    SUN1 (Sun1 Mouse)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available since
    Sept. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    187682-plasmid-map-sequence-id-371222

    Enlarge

  19. pET28-MBP-super TEV protease

    Plasmid
    #171782
    Purpose
    Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.
    Depositor
    Mark Howarth
    Article
    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-
    Insert
    MBP-sTEV
    Use
    Tags
    Arg6 tag and Internal His6 tag
    Expression
    Bacterial
    Mutation
    No internal TEV cleavage site between the MBP and…
    Promoter
    T7
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171782-plasmid-map-sequence-id-443047

    Enlarge

  20. pMDLg/pRRE

    Plasmid
    #12251
    Purpose
    3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)
    Depositor
    Didier Trono
    Article
    Dull et al J Virol. 1998 Nov . 72(11):8463-71.
    Insert
    HIV-1 GAG/POL
    Use
    Lentiviral; Packaging
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12251-plasmid-map-sequence-id-433915

    Enlarge

Showing: 521 - 540 of 150392 results