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Plasmid#108294PurposepX459 V2.0 (Plasmid #62988) with the N692A, M694A, Q695A, and H698A mutationsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only
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pAcGFP1-N1-Dsup
Plasmid#90020PurposeTo express GFP-tagged Dsup in mammalian cells transientlyDepositorInsertDsup
UseTagsAcGFP1ExpressionMammalianMutationPromoterCMVAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct-mCitrine
Plasmid#155220PurposeMonitor changes in the density of membrane-proximal F-actin (MPA)DepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with the CaaX sequence derived from Kras4b
UseTagsmCitrineExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYC-Skp-TF
Plasmid#83921PurposeCo-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.DepositorInsertsSkp
Trigger Factor
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEvol-pAcFRS.2.t1
Plasmid#73544PurposetRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAcFRS.2.t1
pAcFRS.2.t1
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H189
Plasmid#170350PurposemT2Del_EPACdDEPCD_Q270E_tdBlackcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertmT2Del_EPACdDEPCD_Q270E_tdBlackcp173Ven(ST) (RAPGEF3 Human)
UseTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H90
Plasmid#170340PurposeCFP(UST)_EPACdDEPCD_cp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertCFP(UST)_EPACdDEPCD_cp173Venus(ST) (RAPGEF3 Human)
UseTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSSRB070_pAC8-hsCRBN
Plasmid#218789PurposeInsect cell expression vector for FLAG-TEV-Spy-hsCRBNDepositorInsertProtein cereblon (CRBN Human)
UseTagsFlag-TEV-SpyExpressionInsectMutationPromoterpolyhedrinAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCambia1300-PacI
Plasmid#44183DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-C2-hSphK2
Plasmid#84370PurposeExpression of AcGFP-SphK2 fusion protein in mammalian cellsDepositorInsertSPHK2 (SPHK2 Human)
UseTagsAcGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2017AvailabilityAcademic Institutions and Nonprofits only