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Plasmid#203878PurposepSU20 with addition of CEN6-ARSH4-HIS3 cassette for S. cerevisiae and RK2 origin of conjugal transferDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only
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pLKO-PHB-4
Plasmid#122231PurposeExpresses shRNA targeting the 3' UTR of human PHBDepositorInsertPHB (PHB1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFUGW hUbC-Sga-dCas9-KRAB-T2A-GFP
Plasmid#227009PurposeLentiviral Expression of dCas9-KRAB from S. gallolyticus fused to the repressive KRAB domainDepositorInsertdCas9
UseCRISPR and LentiviralTags3xFLAG, SV40 NLSExpressionMutationPromoterUbCAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFUGW hUbC-Sin-dCas9-KRAB-T2A-GFP
Plasmid#227010PurposeLentiviral Expression of dCas9-KRAB from S. iniae fused to the repressive KRAB domainDepositorInsertdCas9
UseCRISPR and LentiviralTags3xFLAG, SV40 NLSExpressionMutationPromoterUbCAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFUGW hUbC-Spa-dCas9-KRAB-T2A-GFP
Plasmid#227011PurposeLentiviral Expression of dCas9-KRAB from S. parasanguinis fused to the repressive KRAB domainDepositorInsertdCas9
UseCRISPR and LentiviralTags3xFLAG, SV40 NLSExpressionMutationPromoterUbCAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFUGW hUbC-Sub-dCas9-KRAB-T2A-GFP
Plasmid#227012PurposeLentiviral Expression of dCas9-KRAB from S. uberis fused to the repressive KRAB domainDepositorInsertdCas9
UseCRISPR and LentiviralTags3xFLAG, SV40 NLSExpressionMutationPromoterUbCAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_mNG2(11)1-10
Plasmid#82610PurposeLentiviral vector of constitutive expression of mNeonGreen2(1-10)DepositorInsertmNeonGreen2(1-10)
UseLentiviralTagsExpressionMammalianMutationK128M, S142T, R150M, G172V and K213MPromoterSFFVAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
TadCBE-Cas9NG
Plasmid#225093PurposePlasmid for bacterial purification of codon-optimized TadCBE-Cas9NGDepositorInsertTadCBE-Cas9NG
UseCRISPRTags8xHisExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Myr_mCherry-2A-eGFP
Plasmid#194877PurposeRatiometric Myr/Palm_mCherry control probeDepositorInsertMyristoylated/Palmitoylated_mCherry-T2A-GFP
UseAAVTagsExpressionMammalianMutationPromoterhuman SynapsinAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
rEstus
Plasmid#209767PurposeTo express a bright and sensitive genetically encoded voltage indicator in mammalian cells.DepositorArticleInsertrEstus
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_CloGFP(1-10)
Plasmid#157994PurposeLentiviral vector of constitutive expression of CloGFP(1-10)DepositorInsertCloGFP(1-10)
UseLentiviralTagsExpressionMammalianMutationG32Q, F46L, K101R, S202LPromoterSFFVAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFD5-NS
Plasmid#149546PurposeExpression of pegRNA(s) and sgRNA(s) under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.DepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterU6Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-eGFP-2A-mCherry_D4H
Plasmid#194875PurposeRatiometric D4H sensor for in vivo neuron-specific cholesterol visualizationDepositorInsertGFP-T2A-mCherry-D4H
UseAAVTagsExpressionMammalianMutationPromoterhuman SynapsinAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-eGFP-2A-mCherry_YDA
Plasmid#194876PurposeRatiometric YDA sensor for in vivo neuron-specific cholesterol visualizationDepositorInsertGFP-T2A-mCherry-YDA
UseAAVTagsExpressionMammalianMutationPromoterhuman SynapsinAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLCNICK
Plasmid#84653PurposeGenome editing for Lactobacillus casei Lc2WDepositorInsertsCas9 nickase
sgRNA
homology arms of LC2W_2179
UseCRISPRTagsExpressionMutationD10APromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET_mNG2(1-10)_32aalinker_mNG2(11)
Plasmid#82611PurposeBacterial expression vector for split mNeonGreen2 screeningDepositorInsertmNeonGreen2(1-10)_32aalinker_mNeonGreen2(11)
UseTagsExpressionBacterialMutationsplit before mutationsPromoterT7Available sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAdTrack-CMV
Plasmid#16405PurposeShuttle vector for us in AdEasy System. For expression of transgenes under a CMV promoter when a GFP tracer is desired.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseAdenoviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Blasticidin
Plasmid#75085Purposeretrovirus-mediated gene transfer into mammalian cellsDepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_mNG3K(1-10)
Plasmid#157993PurposeLentiviral vector of constitutive expression of mNeonGreen3K(1-10)DepositorInsertpSFFV_mNG3K(1-10)
UseLentiviralTagsExpressionMammalianMutationG27D, D42E, M128I, K153M, T170IPromoterSFFVAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSPTg.T2FpAXK (#52)
Plasmid#35962DepositorInsertsTie2 promoter
SV40 pA
Tie2 enhancer
UseTagsExpressionMutationPromoterAvailable sinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only