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pcDNA3-DN-hCUL4B-FLAG
Plasmid#15822DepositorInsertCullin4B (CUL4B Human)
UseTagsFlagExpressionMammalianMutationDominant negative: N594PromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
TFORF2939
Plasmid#144415PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertHOXA6 (HOXA6 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1‐hsRNASEH2BCA
Plasmid#108692PurposeFor expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzymeDepositorUseTagsGSTExpressionBacterialMutationShine Dalgarno sequence upstream of start codon a…PromotertacAvailable sinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-N EBNA1
Plasmid#37954DepositorInsertEBNA1 (EBNA-1 H. Herpesvirus 4 (EBV))
UseRetroviralTagsFlag and HAExpressionMammalianMutationPromoterPKGAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCI HA NEDD4
Plasmid#27002DepositorInsertNEDD4 (NEDD4 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
TFORF0805
Plasmid#141710PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSMARCC2 (SMARCC2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 IL6
Plasmid#67061PurposeStandard for cell-free expression benchmarking.DepositorInsertIL6 (IL6 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mTagBFP2
Plasmid#122373PurposeExpresses mTagBFP2 in mammalian cellsDepositorInsertmTagBFP2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV
Plasmid#216156PurposeExpresses mScarlet regardless of TDP-43 knockdown.DepositorInsertmScarlet with C-terminal FLAG tag
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_JEV_prME
Plasmid#207283PurposeMammalian expression of Japanese encephalitis virus PrME proteinDepositorInsertJEV_PrME (POLY Japanese encephalitis virus)
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
B2380_BTB-IDR
Plasmid#215098PurposeExpresses BCL6 BTB(1-129)-mCherry-FUS (IDR) in mammalian cellsDepositorInsertBTB-mCherry-FUS(IDR)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV flag ERR alpha
Plasmid#10975DepositorInsertERR alpha (ESRRA Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceNov. 23, 2005AvailabilityAcademic Institutions and Nonprofits only -
ACBE
Plasmid#204607PurposeMammalian expression of adenine-to-cytosine base editor ACBEDepositorInsertbpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
UseCRISPRTags6*HisExpressionMammalianMutationmAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)PromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
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mEmerald-LaminB1-10
Plasmid#54140PurposeLocalization: Nuclear Envelope, Excitation: 487, Emission: 509DepositorInsertLaminB1 (LMNB1 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRDA_355
Plasmid#187159PurposeLentiviral expression of doxycycline inducible customizable Sp Cas9 sgRNADepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-enCas12a-2xNLS
Plasmid#209021PurposeLentiviral vector expressing human codon-optimized enCas12a with 2xNLSDepositorInsertenCas12a-2xNLS-Myc-NeoR
UseCRISPR and LentiviralTagsMYCExpressionMammalianMutationE174R/S542R/K548RPromoterEF-1aAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_PUUV_GP
Plasmid#207301PurposeMammalian expression of Puumala virus GP proteinDepositorInsertPUUV_GP
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Puro-CAG-mNG2(1-10)
Plasmid#206042PurposeRepair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.DepositorInsertAAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette
UseCRISPR and Synthetic Biology; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ H2B-mTagBFP2
Plasmid#99267PurposeContains a fusion of human histone H2B to fluorescent protein mTagBFP2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.DepositorInsertmTagBFP2-3XHA
UseTagsH2BExpressionMammalianMutationPromoterSP6Available sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-hPAX3 (#395)
Plasmid#78335PurposeOverexpression of human PAX3 ORF with a N-terminal FLAG tagDepositorInsertPAX3 (PAX3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCI152-pLenti-SiT-Cas12a
Plasmid#128405PurposeExpress both AsCas12a and CRISPR arrays on single transcriptsDepositorInsertAsCas12a-Triplex
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
B2345_BTB2x-mCherry
Plasmid#215097PurposeExpresses BCL6 BTB(1-129)-BTB(1-129)-mCherry in mammalian cellsDepositorInsertBCL6 BTB-BTB (BCL6 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLI50
Plasmid#13573DepositorTypeEmpty backboneUseS. aureus-e. coli shuttle vectorTagsExpressionMutationPromoterAvailable sinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
ND6-DdCBE-left side TALE
Plasmid#157842Purposeexpresses left-side TALE–DddAtox half of ND6-DdCBE in mammalian cellsDepositorInsertSOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–SOD2 3'UTR
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
p-eGFP-beta 2 CP
Plasmid#13298DepositorInsertcapping protein beta 2 subunit (Capzb Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
Fv-hCaspase 8-2A-GFP
Plasmid#82712Purposeexpress human caspase 8 in mammalian cellsDepositorInsertFv-Caspase 8-2A-GFP (CASP8 Human)
UseRetroviralTagsFKBP dimerization domainExpressionMammalianMutationdeleted amino acids 1-215 of caspase 8PromoterLTRAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG Luciferase
Plasmid#98391PurposeGateway entry vector for inducible expression of Luciferase with N-terminal 3xFLAG tagDepositorInsertLuciferase
UseEntry vector for gateway cloningTags3xFLAGExpressionMutationPromoterTRE tight and TRE tight promoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TRPV1exCellHalo
Plasmid#220307PurposeMammalian expression vector for a subunit of rat TRPV1 with circularly permutated HaloTag inserted between the S5 and S6 transmembrane helicesDepositorInsertrTRPV1exCellHalo (Trpv1 Rat)
UseTagscircularly permutated Halotag (Deo et al., Nat Ch…ExpressionMammalianMutationlinker included to insert cpHaloTag (PromoterCMV promoterAvailable sinceJuly 30, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits
