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Plasmid#153282PurposeEnd-linker for cloning 4 sgRNA arrays into any recipient vectorDepositorInsertlinker
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Smad1 EVE GM
Plasmid#27997DepositorInsertHuman Smad1 EVE Gsk3 mutant (SMAD1 Human)
UseTagsFlagExpressionMammalianMutationS463E S465E S210A T202A S191A S198APromoterAvailable sinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMNK55 - piRNAi 21ur-1224 - cku-80
Plasmid#159820PurposepiRNA-induced RNA interference (piRNAi) to bias double-strand repair toward homologous recombinationDepositorInsertcku-80 piRNAi 21ur-1224
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMNK54 - piRNAi 21ur-1224 - him-5
Plasmid#159818PurposepiRNA-induced RNA interference (piRNAi) to induce males in transgenic animalsDepositorInserthim-5 piRNAi 21ur-1224
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMNK56 - piRNAi 21ur-1224 - him-8
Plasmid#159819PurposepiRNA-induced RNA interference (piRNAi) to induce males in transgenic animalsDepositorInserthim-8 piRNAi 21ur-1224
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-SGTAP no-pA
Plasmid#82599Purpose3' entry vector containing streptavidin binding protein (SBP) followed by a TEV cleavage site and 2 protein G copies without pA for tandem affinity purificationDepositorInsertSBP-TEV-2x protein G
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSB4k5_RNAnano (r-plasmid)
Plasmid#127634PurposeEncodes an RNA nanostructure with MG, PP7, TAR and S1m RNA aptamers. Transcription via T7 RNAP.DepositorInsertRNA nanostrcutre
UseSynthetic BiologyTagsExpressionMutationPromoterT7 RNAPAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGE655
Plasmid#153253PurposeM1 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE656
Plasmid#153254PurposeM2 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE658
Plasmid#153256PurposeM3 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR2-L3-SfoI-EGFP-secNanoLuc-PBL-R1
Plasmid#141025PurposeMutisite Gateway mediated vector constructionDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L1-PGK-Neo-L2
Plasmid#141007PurposeMutisite Gateway mediated vector constructionDepositorInsertNeomycin resistance gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEU3-NII-HLICNot
Plasmid#140181PurposeUsed for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, 6x His-tag and NotI restriction siteDepositorTypeEmpty backboneUseExpression vector for a cell-free systemTagspEU3-NII-HLICNotExpressionMutationPromoterT7Available sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAct:MS2-p65-HSF
Plasmid#78902PurposeExpresses MS2-p65-HSF under actin promoter for SAM CRISPRa in Drosophila cellsDepositorInsertMS2-p65-HSF
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)Available sinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCSN069
Plasmid#166728PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-Mxi1
UseCRISPRTagsMxi1ExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-MS2tail-SAM
Plasmid#78905PurposeVector for expressing a single sgRNA with SAM MS2 tail, under U6:3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceAug. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3E-Dam-myc no-pA
Plasmid#82600Purpose3' entry vector containing myc epitope tag fused to C-terminus of E. coli DNA adenine methyltransferase without pA for DamIDDepositorInsertDam-Myc (no pA)
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHSP70B-CP6-hph
Plasmid#165879PurposeBasic Hygromycin resistance cassette in Alpha1, lacks physical marker or attB siteDepositorInsertBasic Selection Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEE386
Plasmid#149284PurposeT-DNA encoding TRV2 with gRNA targeting NbAGDepositorInsertp35S:TRV2_NbAGsgRNA1:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEE388
Plasmid#149286PurposeT-DNA encoding TRV2 with mFT augmented gRNA targeting NbAGDepositorInsertp35S:TRV2_NbAGsgRNA1_mFT:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only