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Plasmid#201533PurposeExpressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE_RBS3_CB2
UseSynthetic BiologyTagsExpressionMutationrhaR L128+1bp (frameshift), ilvA G455CPromoterPrhaBAD (rhamnose)Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24_T24_pRham-ilvA-relE_RBS3_CB4
Plasmid#201535PurposeExpressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE_RBS3_CB4
UseSynthetic BiologyTagsExpressionMutationrhaS ΔM1-H5, PrhaSR Δ179bpPromoterPrhaBAD (rhamnose)Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEXPA10-NLS-3xmCitrine
Plasmid#192523PurposeFluorescent reporter for plant responses to bacterial infectionDepositorInsertEXPANSIN A10 (EXPA10 Mustard Weed)
UseTags3xmCitrine and NLSExpressionPlantMutationPromoterEXPA10Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
MT2A_beta-domain
Plasmid#200300PurposeBacterial expression plasmids for production of recombinant beta-domain of metallothionein 2aDepositorInsertbeta-domain of metallothionein 2a (MT2A Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterT7Available sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
MT2A_alpha-domain
Plasmid#200302PurposeBacterial expression plasmids for production of recombinant alpha-domain of metallothionein 2aDepositorInsertalpha-domain of metallothionein 2a (MT2A Human)
UseTagsChiting Binding Domanin-InteinExpressionBacterialMutationPromoterT7Available sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-HiBIT-WDR5
Plasmid#200883PurposeBacterial expression of N-terminal HiBIT-tagged human WDR5 proteinDepositorInsertHiBIT-WDR5 (WDR5 Human)
UseTagsExpressionBacterialMutationWTPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
SuExp-mPLD3
Plasmid#201245Purposeexpress full length mouse PLD3 proteinDepositorInsertPLD3 (Pld3 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF-pMB1'- BAD-YFP
Plasmid#186567PurposepMB1' ori, L-arabinose PBAD promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
UseTagsExpressionBacterialMutationPromoterBAD promoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMe31B-QHTKRKKtoAAAAAAA_AC
Plasmid#148386PurposeInsect Expression of DmMe31B-QHTRKKtoAAAAAADepositorInsertDmMe31B-QHTRKKtoAAAAAA (me31B Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMe31B-R307AK313AK314A_AC
Plasmid#148387PurposeInsect Expression of DmMe31B-R307AK313AK314ADepositorInsertDmMe31B-R307AK313AK314A (me31B Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF-p15A-trc- YFP
Plasmid#186569Purposep15A ori, lacI, Ptrc promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
UseTagsExpressionBacterialMutationPromotertrc promoterAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF-p15A-T7- YFP
Plasmid#186568Purposep15A ori, lacI PT7 promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-Rheb(5A)
Plasmid#192439PurposeEncodes mCherry-tagged Rheb lacking residues 38-42.DepositorInsertmCherry-Rheb(C181S) (RHEB Human)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationRheb amino acids 38-42 deleted.PromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjT-tdTomato-PGK-Neo
Plasmid#186173PurposeKnock-in gene targeting in marmoset cells at TBXT locusDepositorInserttdTomato
UseMarmoset targetingTagsExpressionMutationPromoterAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjOSR1-EGFP-PGK-Puro
Plasmid#186170PurposeKnock-in gene targeting in marmoset cells at OSR1 locusDepositorInsertEGFP
UseMarmoset targetingTagsExpressionMutationPromoterAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjSF1-tdTomato-PGK-Neo
Plasmid#186172PurposeKnock-in gene targeting in marmoset cells at SF1 locusDepositorInserttdTomato
UseMarmoset targetingTagsExpressionMutationPromoterAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSF-pMB1'- trc-YFP
Plasmid#186565PurposepMB1' ori, lacI, Ptrc promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
UseTagsExpressionBacterialMutationPromotertrc promoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSF-pMB1'- tac-YFP
Plasmid#186566PurposepMB1' ori, lacI, Ptac promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expressionDepositorInsertYFP
UseTagsExpressionBacterialMutationPromotertac promoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNL(CMV)Rep78-III/CMV/WPREΔU3
Plasmid#190726PurposeExpression of AAV2 Rep78-III in mammalian cellsDepositorInsertAAV2 Rep78-III
UseLentiviralTagsExpressionMammalianMutationPromoterCMV-IEAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNL(CMV)Rep78-III/CAG/WPREΔU3
Plasmid#190727PurposeExpression of AAV2 Rep78-III in mammalian cellsDepositorInsertAAV2 Rep78-III
UseLentiviralTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only