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Plasmid#23092DepositorInsertBim EL (TD) (Bcl2l11 Mouse)
UseTagsFlagExpressionMammalianMutationChanged Threonine 112 to AspartatePromoterAvailable sinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-scFv
Plasmid#78900PurposeExpresses scFv-VP64 under actin promoter for SunTag CRISPRa in Drosophila cellsDepositorInsertscFV-VP64
UseCRISPRTagsGFPExpressionInsectMutationPromoterpActin (Drosophila)Available sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
CeNL-Clathrin_pEGFP
Plasmid#89540PurposeCyan color variant of bright luminescent protein, fused to clathrin light chainDepositorInsertCeNL
UseTagsClathrin light chainExpressionMammalianMutationPromoterCMVAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
Rc/CMV cyclin D1 K112E HA
Plasmid#8952DepositorInsertcyclin D1 (CCND1 Human)
UseTagsHAExpressionMammalianMutationK112E: point mutation in cyclin boxPromoterAvailable sinceOct. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pBRC169
Plasmid#79670PurposeDsREDmonomer-based BiFC vector for transient expression of RC (169-225) in plants.DepositorTypeEmpty backboneUseReporter plasmidTagsDsRED-monomer (169–225)/RC169ExpressionPlantMutationPromoterCaMV35SAvailable sinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBGN155
Plasmid#74992PurposeGFP(S65T)-based BiFC vector for transient expression of sGN (1-155) in plantsDepositorTypeEmpty backboneUseReporter plasmidTagsGFP (1–155)/GN155ExpressionPlantMutationPromoterCaMV35SAvailable sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBDR
Plasmid#62663PurposeDonor vector for tdTomato knock-in via landing padDepositorInserttdTomato
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NLS-ZapCmR1.1
Plasmid#59011PurposeNuclear zinc-binding FRET biosensorDepositorInsertNLS-ZapCmR1.1 genetically encoded Zn(II) sensor
UseTagsSV40 NLSExpressionMammalianMutationSee commentPromoterCMVAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
VV221: sdChR(C138S)-TS-eGFP-ER in fck
Plasmid#58513PurposeExpresses sdChR with the C138S (step function opsin) mutation fused to eGFP under the a-CamKII promoterDepositorInsertsdChR(C138S)-TS-eGFP-ER
UseLentiviralTagseGFPExpressionMammalianMutationchanged Cysteine 138 to SerinePromotera-CamKIIAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEE389
Plasmid#149287PurposeT-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbAGDepositorInsertp35S:TRV2_NbAGsgRNA1_TrunFT:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEE387
Plasmid#149285PurposeT-DNA encoding TRV2 with FT augmented gRNA targeting NbAGDepositorInsertp35S:TRV2_NbAGsgRNA1_FT:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-TAPP1-PH
Plasmid#67675PurposeMultisite gateway entry clone for adding C-terminal fusions of TAPP1-PHDepositorInsertTAPP1-PH
UseGateway multisite 3' entry cloneTagsExpressionMutationPromoterAvailable sinceJune 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA
Plasmid#87560PurposeExpresses aa397-423 of Flp recombinase fused to PYL CIDDepositorInsertFlpO aa397-423
UseCre/Lox and Synthetic BiologyTagsPYLExpressionMammalianMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA
Plasmid#87559PurposeExpresses aa1-396 of Flp recombinase fused to ABI CIDDepositorInsertFlpO aa1-396
UseCre/Lox and Synthetic BiologyTagsABIExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3E-FRB(PLF)-HA no-pA
Plasmid#82597Purpose3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (K2095P, T2098L, W2101F) without pA for rapamycin/rapalog induced dimerizationDepositorInsertFRB-HA (no pA)
UseTagsExpressionMutationK2095P, T2098L, W2101FPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
R57C10-Flp2
Plasmid#63172PurposeR57C10 (N-synaptobrevin), "Pan-Neuronally," driven wild type yeast Flp2 promoter-fusionDepositorInsertyeast wild type containing a Glycine at amino acid 5 of the protein sequence
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPB-DEST-R1R2-pNeo
Plasmid#141057PurposeMutisite Gateway mediated vector constructionDepositorInsertNeomycin resistance gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA1 WT
Plasmid#66139PurposeExpresses the wild type of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAGM55361
Plasmid#153227PurposeControl plasmid for knockout of AP3 geneDepositorInsertCas9, guide RNA for TRY and CPC and FAST marker
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE509
Plasmid#153281PurposeEnd-linker for cloning 3 sgRNA arrays into any recipient vectorDepositorInsertlinker
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only