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Plasmid#186660PurposeDonor plasmid to endogenously tag VIR gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.DepositorInsertOSS VIR C-terminal 3xFlag-3xHA Tag Donor Plasmid (vir Fly)
UseTags3xFlag-3xHAExpressionInsectMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_N_FH_Shu
Plasmid#186655PurposeDonor plasmid to endogenously tag Shu gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.DepositorInsertOSS Shu N-terminal 3xFlag-3xHA Tag Donor Plasmid (shu Fly)
UseTags3xFlag-3xHAExpressionInsectMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_C_FH_Nbr
Plasmid#186662PurposeDonor plasmid to endogenously tag Nbr gene in Drosophila melanogaster at the C-terminal.DepositorInsertOSS Nbr C-terminal 3xFlag-3xHA Tag Donor Plasmid (Nbr Fly)
UseTags3xFlag-3xHAExpressionInsectMutationPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDonor_N_FH_GLE
Plasmid#186657PurposeDonor plasmid to endogenously tag GLE gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.DepositorInsertOSS Gle N-terminal 3xFlag-3xHA Tag Donor Plasmid (CG14749 Fly)
UseTags3xFlag-3xHAExpressionInsectMutationPromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-Chpt1
Plasmid#175155PurposeLentiviral expression of V5-tagged mouse Chpt1DepositorInsertChpt1 (Chpt1 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Dnajc16-V5
Plasmid#175156PurposeLentiviral expression of mouse Dnajc16-V5DepositorInsertDnajc16 (Dnajc16 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Cdipt-V5
Plasmid#175144PurposeLentiviral expression of mouse Cdipt-V5DepositorInsertCdipt (Cdipt Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-Drosha(W1258)
Plasmid#171535PurposeEncodes mutant human Drosha proteinDepositorInsertDROSHA (DROSHA Human)
UseTagsExpressionMammalianMutationa Drosha stopping at W1258PromoterAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV Myc-emerin
Plasmid#175101PurposeLentiviral expression of Myc-tagged mouse EmdDepositorInsertEmd (Emd Mouse)
UseLentiviralTagsMycExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-Drosha (P426)
Plasmid#171537PurposeEncodes mutant human Drosha proteinDepositorInsertDROSHA (DROSHA Human)
UseTagsExpressionMammalianMutationa Drosha mutant starting from P426PromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-Drosha (E419)
Plasmid#171534PurposeEncodes mutant human Drosha proteinDepositorInsertDROSHA (DROSHA Human)
UseTagsExpressionMammalianMutationa Drosha mutant stopping at E419PromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCK-Drosha-FLAG(delta6)
Plasmid#171532PurposeEncodes mutant human Drosha proteinDepositorInsertDROSHA (DROSHA Human)
UseTagsExpressionMammalianMutationsmall deletions of Drosha_1271A to 1276G_PromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-Dicer-dsRBD (+6)
Plasmid#171544PurposeEncodes a Dicer fragment extending from from R1841 to the C-terminusDepositorInsertDICER1 (DICER1 Human)
UseTagsExpressionBacterialMutationa six amino acid_TRLTEG_insertion in the L1 regionPromoterAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-Tac-STC
Plasmid#162490PurposeExpresses partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cellsDepositorInsertpartial length Tac (stem region, TMD and cytosolic tail) (IL2RA Human)
UseTagsGFPExpressionMammalianMutationTac is in partial length with its stem region, TM…PromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
BE3-YE1
Plasmid#132943PurposeGene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.DepositorInsertBE3-(W90Y-R126E)
UseCRISPR; Base editorTagsExpressionMammalianMutationBE3-(W90Y-R126E)PromoterAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
human ETV6 gRNA-1
Plasmid#133353Purposehuman ETV6 gRNA-1 is a gRNA expression plasmid. Its 20-nt specific sequence targets the first ETV6 exon.DepositorInsertETV6 (ETV6 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
AdMKP-3 S159AS197A
Plasmid#131523PurposeExpresses MKP-3 with S159A mutation and S197A mutation in mammalian cellsDepositorInsertMitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
UseAdenoviralTagsNoExpressionMutationchanged Serine 159 to Alanine and Serine 197 to A…PromoterCMVAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GABARα6N-HA
Plasmid#128111PurposeExpress HA tagged GABARα6 N terminal domainDepositorInsertGabra6 (Gabra6 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
hsa-cdk2 3'UTR sicheck2 mut
Plasmid#121124Purposeluciferase assayDepositorInserthsa-cdk2 3'UTR mutated 199 binding (CDK2 Human)
UseTagsExpressionBacterialMutationmiR-199 seed binding site mutatedPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tol2-lyzC-mcherry-2a-CDK2-D145N
Plasmid#121132Purposeneutrophil specific expressionDepositorInsertdre-D145N CDK2 DN (cdk2 Zebrafish)
UseTagsmcherry-2aExpressionBacterialMutationD145N point mutation DNPromoterneutrophil specific lyzCAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only