Addgene

We narrowed to 8,439 results for: CAG

Showing: 5321 - 5340 of 8439 results

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pRSFDuet1-sgRNA(siteT9)

Plasmid

#154832

Purpose

sgRNA for CRISPR/Cas9-mediated targeted integration into genomic site T9 in CHO cells

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Insert

sgRNA(siteT9)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-blast shKGA

Plasmid

#110420

Purpose

shKGA, silence glutaminase KGA isoform, blasticidin selection.

Depositor

Sandra Martha Gomes Dias

Insert

GLS glutaminase (GLS Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6 (RNA Pol III)

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pTK73.ChrII_ttTi5605.sgRNA

Plasmid

#194058

Purpose

sgRNA targeting ChrII_ttTi5605 locus of the C. elegans genome

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

ChrII_ttTi5605 sgRNA

Use

Tags

Expression

Worm

Mutation

Promoter

U6

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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VPS35 shRNA1

Plasmid

#163858

Purpose

shRNA targeting the coding sequences of VPS35

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

VPS35 retromer complex component (VPS35 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - AAVS1 sgRNA

Plasmid

#70661

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an AAVS1-targeting sgRNA element from U6 promoter. Lentiviral backbone

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against AAVS1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only
pEX128·gRNA

Plasmid

#187600

Purpose

Template for amplification of gRNA with Cas6 recognition site

Depositor

Pablo Ivan Nikel

Article

Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

Insert

gRNA template

Use

Tags

Expression

Mutation

Promoter

no promoter

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hNoxa1

Plasmid

#58531

Purpose

expresses human Noxa1 in mammalian cells

Depositor

Botond Banfi, Karl-Heinz Krause

Article

Banfi et al J Biol Chem. 2003 Feb 7;278(6):3510-3. E

Insert

NADPH oxidase activator 1 (NOXA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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AAV-shRNA-Tet2

Plasmid

#85743

Purpose

shRNA against mouse Tet2

Depositor

Hongjun Song

Article

Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

Insert

shRNA Tet2

Use

AAV

Tags

EYFP

Expression

Mutation

Promoter

Available since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-NEAT1pr_v2

Plasmid

#97082

Purpose

Encodes an sgRNA that creates a DSB at the promoter of human NEAT1 gene

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1pr_v2

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiX2-PURO-shGFP

Plasmid

#61230

Purpose

Lentivirus expressing shRNA to GFP

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

H1/TO

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
CMV-VARNAM

Plasmid

#115552

Purpose

Red fluorescent voltage indicator for mammalian expression

Depositor

Vincent Pieribone

Article

Kannan et al Nat Methods. 2018 Dec;15(12):1108-1116.

Insert

VARNAM

Use

Tags

Golgi and ER export signal

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO mouse shRNA 2 raptor

Plasmid

#21340

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 13. ():.

Insert

raptor shRNA 2 (Rptor Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-DIO-EF1a-TVA-P2A-EYFP

Plasmid

#177017

Purpose

For expression of the avian TVA receptor in a cre-dependent manner

Depositor

Peter Jonas

Insert

TVA-P2A-EYFP (CD320 Coturnix japonica)

Use

AAV and Cre/Lox

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti‐puro‐OgRNA_mdxE53

Plasmid

#187054

Purpose

SpCas9 gRNA correcting mdx4cv nonsense mutation with ABE

Depositor

Renzhi Han

Article

Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

Insert

SpCas9 gRNA correcting mdx4cv nonsense mutation with ABE

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pX-EGFP-g1

Plasmid

#107273

Purpose

eGFP sgRNA-1 and Cas9 expression vector (aka. pX-ps1)

Depositor

Knut Woltjen

Article

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Insert

GFP sgRNA-1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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ACE2 g2

Plasmid

#153012

Purpose

A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

Depositor

Jason Sheltzer

Insert

ACE2 gRNA (ACE2 Human)

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

Available since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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miniCMV-tdTomado-tDeg

Plasmid

#185402

Purpose

tdTomado-tDeg fluorogenic protein

Depositor

Jian-Hui Jiang

Article

Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

Insert

tdTomado-tDeg

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ2853-3 pHR: U6-Sasgv2CXCR4-1 CMV-EGFP

Plasmid

#84254

Purpose

Expresses optimized Sa sgCXCR4-1 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgCXCR4-1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCY_gRNA_hPAX7-Pro_C3

Plasmid

#160454

Purpose

sgRNA targeting human PAX7 promoter

Depositor

April Pyle

Article

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Insert

gRNA_hPAX7-Promoter_C3

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCrx-DsRed

Plasmid

#13765

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Crx promoter (Crx Mouse)

Use

Tags

DsRed2

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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