Addgene

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JAB2156

Plasmid

#190345

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::AmtR-VP16-19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::AmtR-VP16-19St

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 F355R

Plasmid

#195209

Purpose

Mammalian expression vector for GFP tagged NHERF1. Ezrin interaction-deficient mutant

Depositor

Anthony Bretscher, David Bryant

Article

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Use

Tags

Expression

Mammalian

Mutation

F355R

Promoter

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET19b-HisDAD2

Plasmid

#165996

Purpose

Expression of coding sequence in Escherichia coli

Depositor

Claudia Vickers

Article

Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

Insert

HisDAD2

Use

Tags

6His

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub(G75,76A)-BsmBI

Plasmid

#162803

Purpose

Control STELLA destination plasmid for N-terminally labelled proteins

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub(G75,76A)-BsmBI

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Ubiquitin (G75,76A)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*SMIM38

Plasmid

#162808

Purpose

STELLA plasmid for amber suppression of N-terminally labelled SMIM38

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)SMIM38

Use

Piggybac

Tags

Expression

Mammalian

Mutation

SMIM38 (Met1, Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4x7SKPylT_EF1_Ub-*PIGBOS

Plasmid

#162809

Purpose

STELLA plasmid for amber suppression of N-terminally labelled PIGBOS

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)PIGBOS

Use

Piggybac

Tags

Expression

Mammalian

Mutation

PIGBOS (Met1, Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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hSDH5_C83D

Plasmid

#113048

Purpose

Expression of mutant hSDH5

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

hSDH5_C83D (SDHAF2 Human)

Use

Tags

Expression

Bacterial

Mutation

C83D, Insert begins at amino acid 39

Promoter

Available since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN11 (L1L2)

Plasmid

#80111

Purpose

Palm1-Late1(delta)-I3-01-myc

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-11 (L1L2)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
Mouse NCKX4-AS-short (pcDNA3.1+)

Plasmid

#75207

Purpose

Mammalian expression of SLC24A4

Depositor

Jonathan Lytton

Article

Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

Insert

SLC24A4 (Slc24a4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

alternatively spliced exon absent

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pDHJS3 AN+

Plasmid

#78240

Purpose

One of four plasmids needed to create the mismatched double Holliday junction substrate (MM-DHJS).

Depositor

Tao-shih Hsieh

Article

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "A" with NheI site
loxP2

Use

Phagemid

Tags

Expression

Mutation

Promoter

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDHJS3 BS-

Plasmid

#78239

Purpose

One of four plasmids needed to create the double Holliday junction substrate (DHJS).

Depositor

Tao-shih Hsieh

Article

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "A"
loxP2

Use

Phagemid

Tags

Expression

Mutation

Promoter

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDHJS3 AS+

Plasmid

#78236

Purpose

One of four plasmids needed to create the double Holliday junction substrate (DHJS).

Depositor

Tao-shih Hsieh

Article

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "A"
loxP2

Use

Phagemid

Tags

Expression

Mutation

Promoter

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDHJS3 BS+

Plasmid

#78252

Purpose

One of four plasmids needed to create the double Holliday junction substrate (DHJS).

Depositor

Tao-shih Hsieh

Article

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "B"
loxP2

Use

Phagemid

Tags

Expression

Mutation

Promoter

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B11-Leum

Plasmid

#44319

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B11-Leum promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B11-Leum

Available since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B1-Leum

Plasmid

#44303

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B1-Leum promoter

Use

Synthetic Biology

Tags

N/A

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B1-Leum

Available since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B2-Leum

Plasmid

#44310

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B2-Leum promoter

Use

Synthetic Biology

Tags

N/A

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B2-Leum

Available since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B3-Leum

Plasmid

#44311

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B3-Leum promoter

Use

Synthetic Biology

Tags

N/A

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B3-Leum

Available since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pEXP5-NT-AlleyCat

Plasmid

#41715

Depositor

Ivan Korendovych, William DeGrado

Article

Korendovych et al Proc Natl Acad Sci U S A. 2011 Apr 26;10

Insert

mutated C-terminal portion of calmodulin gene (M76-K148)

Use

Tags

Polyhistidine (6×His) region, TEV recognition sit…

Expression

Bacterial

Mutation

F92E

Promoter

T7

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B9-Leum

Plasmid

#44317

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B9-Leum promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B9-Leum

Available since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B7-Leum

Plasmid

#44315

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B7-Leum promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B7-Leum

Available since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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