Skip to main content
Addgene
Showing: 5301 - 5320 of 13536 results

  5. pOPO685

    Plasmid
    #195300
    Purpose
    pBAD322-MycaIDCas-PaqCI, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is replaced with PaqCI entry site.
    Depositor
    Joseph Peters
    Article
    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765
    Insert
    McCAST Cascade and native array with PaqCI entry site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Arabinose inducible arapBAD
    Available since
    May 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    195300-plasmid-map-sequence-id-394739

    Enlarge

  13. pCytERM-hfYFP

    Plasmid
    #186520
    Purpose
    Visualization of the ER in cells using hyperfolder YFP (compatible with OSER assay)
    Depositor
    Gregory Petsko
    Article
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Insert
    Hyperfolder YFP
    Use
    Tags
    Cytochrome p450 first 29 animo acids (Oryctolagus…
    Expression
    Mammalian
    Mutation
    mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…
    Promoter
    CMV
    Available since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186520-plasmid-map-sequence-id-368218

    Enlarge

  15. pOPO717

    Plasmid
    #195302
    Purpose
    pBAD322-MycaIDCas-lacZsp5, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is lacZ spacer 5.
    Depositor
    Joseph Peters
    Article
    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765
    Insert
    McCAST Cascade and lacZ spacer 5 in native array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Arabinose inducible arapBAD
    Available since
    March 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    195302-plasmid-map-sequence-id-383138

    Enlarge

  16. pSYN-VWF-039

    Plasmid
    #120292
    Purpose
    VWF expression construct expressing D'D3 domains with C1099A/C1142A mutations in D3 domain as an Fc fusion protein
    Depositor
    Robert Peters
    Article
    Dong et al Blood. 2019 Jan 14. pii: blood-2018-10-8
    Insert
    VWF D1-D2-D'-D3-Fc
    Use
    Tags
    Fc
    Expression
    Mammalian
    Mutation
    C1099A/C1142A
    Promoter
    CMV
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    120292-plasmid-map-sequence-id-233304

    Enlarge

  20. pOCC110

    Plasmid
    #118905
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C, and C-terminal sortase site (LPETGg)
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-NotI-ccdB-AscI-LPETGg-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease and LPETGg
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118905-plasmid-map-sequence-id-232327

    Enlarge

Showing: 5301 - 5320 of 13536 results