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pACUH-sfcherry2(1-10)
Plasmid#172061PurposeUAS vector to express sfcherry2(1-10) in DrosophilaDepositorInsertsfcherry2(1-10)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Smad5-3'UTR
Plasmid#61777PurposeLuciferase reporter containing the 3'UTR of mouse Smad5DepositorInsertmouse Smad5 3'UTR
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMZ-347
Plasmid#195458PurposeLentiviral Integratable, constitutive expression of synZiFTR: ZF10-p65DepositorInsertpSFFV - 3XFLAG - NLS - ZF1 - p65
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLOT-syb-sp-YFP1-10
Plasmid#73700PurposeNeuronal-synaptobrevin (syb):spYFP1–10 chimera for synaptic GRASPDepositorInsertNeuronal-synaptobrevin:spYFP1–10 chimera
UseLexaop reporter vectorTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgSmad4/Cre
Plasmid#89651PurposeExpresses a Smad4-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Smad4 (Smad4 Mouse)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBP-J23100
Plasmid#72963PurposeLevel 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion)DepositorInsertJ23100 promoter
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgSetd2#1/Cre
Plasmid#89649PurposeExpresses a Setd2-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Setd2 (Setd2 Mouse)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-PAmCherry1-MCS
Plasmid#60608PurposeCloning PAmCherry1DepositorInsertPAmCherry1
UseCloningTagsExpressionMutationPromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
p3E-EGFP-NS-MCS
Plasmid#75180PurposeEGFP (green) fluorophore with multiple cloning site for use with inducible N-terminal fusions.DepositorInsertEGFP-NS-MCS
UseThree prime entry vector for multisite gateway th…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEE390
Plasmid#149280PurposeT-DNA encoding TRV2 with gRNA targeting NbPDS3DepositorInsertp35S:TRV2_NbPDS3sgRNA:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSN074
Plasmid#166731PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS
UseCRISPRTagsSV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-sGFP
Plasmid#79508PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Super GFP CDSDepositorInsertsuperGFP
UseMultisite gateway entry cloneTags6xHisExpressionMutationPromoterno PromoterAvailable sinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3E-FRB-HA no-pA
Plasmid#82594Purpose3' entry vector containing HA epitope fused to C-terminus of TOR FKBP and rapamycin binding (FRB) domain without pA for rapamycin/rapalog induced dimerizationDepositorInsertFRB-HA (no pA)
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
HisSumoOphP
Plasmid#189672PurposeHisSUMO tagged OphP to express OphP in pichia pastorias.DepositorInsertHisSumoOphP
UseTagsHisSumo taggedExpressionYeastMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rc/CMV cyclin D1 K112E
Plasmid#8951DepositorInsertcyclin D1 (CCND1 Human)
UseTagsExpressionMammalianMutationK112E: point mutation in cyclin boxPromoterAvailable sinceOct. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
cr3Dd
Plasmid#184862Purposecr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination systemDepositorInsertCas9, bet, exo
UseTagsCas9 ssrA degradation tagExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2B Flag BimEL(TA)
Plasmid#23093DepositorInsertBim EL (TA) (Bcl2l11 Mouse)
UseTagsFlagExpressionMammalianMutationChanged Threonine 112 to AlaninePromoterAvailable sinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141C2.0
Plasmid#99905PurposeExpress single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU6Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-ZapCmR1.1
Plasmid#59014PurposeCytosolic zinc-binding FRET biosensorDepositorInsertNES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor
UseTagsNuclear Exclusion SignalExpressionMammalianMutationSee commentPromoterCMVAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only
