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Plasmid#221749PurposeStable expression of WIPI2b-mRuby3 in mammalian cellsDepositorInsertWIPI2b (WIPI2 Human)
UseRetroviralTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-FAPP(PHD)
Plasmid#221748PurposeStable expression of GFP-FAPP(PHD) in mammalian cellsDepositorInsertFAPP (PLEKHA3 Human)
UseRetroviralTagsGFPExpressionMammalianMutationamino acids 1–101PromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-OSBP(PHD)
Plasmid#221745PurposeStable expression of GFP-OSBP(PHD) in mammalian cellsDepositorInsertOSBP (OSBP Human)
UseRetroviralTagsGFPExpressionMammalianMutationamino acids 87–185PromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIB-mRuby3-STX17TM
Plasmid#221744PurposeStable expression of mRuby3-STX17TM in mammalian cellsDepositorInsertSTX17 (STX17 Human)
UseRetroviralTagsmRuby3ExpressionMammalianMutationdeleted amino acids 1-228PromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAC3_C_CCDC22(Y557C)
Plasmid#209695PurposeExpresses MAC3-tagged CCDC22(Y557C) in mammalian cellsDepositorInsertCCDC22 (CCDC22 Human)
UseTagsMAC3-tagExpressionMammalianMutationY557C, changed Tyrosine 557 to CysteinePromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAC3_C_CCDC22(T17A)
Plasmid#209694PurposeExpresses MAC3-tagged CCDC22(T17A) in mammalian cellsDepositorInsertCCDC22 (CCDC22 Human)
UseTagsMAC3-tagExpressionMammalianMutationT17A, changed Threonine 17 to AlaninePromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr
Plasmid#218767Purposeencodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAGDepositorInsertEcTyrRS-pAAFRS-9
UseTagsExpressionBacterialMutationY37G, L71V, D182C, F183Y, L186C, D265RPromotertacIAvailable sinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGex-6p2_NSD2
Plasmid#221376PurposeExpresses NSD2 in bacterial cellsDepositorInsertHistone-lysine N-methyltransferase NSD2 (NSD2 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGex-6p2_ATRX_K1033R
Plasmid#221378PurposeExpresses ATRX_K1033R in bacterial cellsDepositorInsertTranscriptional regulator ATRX (ATRX Human)
UseTagsExpressionBacterialMutationChanged lysine 1033 to argininePromotertacAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGex-6p2_FANCM
Plasmid#221379PurposeExpresses FANCM in bacterial cellsDepositorInsertFanconi anemia group M protein (FANCM Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGex-6p2_FANCM_K819R
Plasmid#221380PurposeExpresses FANCM_K819R in bacterial cellsDepositorInsertFanconi anemia group M protein (FANCM Human)
UseTagsExpressionBacterialMutationChanged lysine 819 to argininePromotertacAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEYFP_C1_ATRX
Plasmid#221383PurposeExpresses ATRX in mammalian cellsDepositorInsertTranscriptional regulator ATRX (ATRX Human)
UseTagsExpressionMammalianMutationK536N (Please see depositor comments)PromoterCMVAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSK519-cspB
Plasmid#207162PurposeExpresses codon-optimized cspB from Clavibacter michiganensis in a broad host range vectorDepositorInsertcspB
UseTagsHAExpressionBacterialMutationPromoternptIIAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xMS2 TR knockin sgRNA 1
Plasmid#207607PurposesgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locusDepositorInsertcgactcgcccggcagcgcac
UseTagsNoneExpressionMammalianMutationPromoterCMV and U6Available sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xMS2 TR knockin sgRNA 2
Plasmid#207608PurposesgRNA 2 for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locusDepositorInsertacccccaaacctgactgact
UseTagsNoneExpressionMammalianMutationPromoterCMV and U6Available sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-myr-rsEGFP2- LDLR
Plasmid#213399Purposedouble floxed, myristoylation site (myr) and LDLR, fused to reversibly switchable EGFP rsEGFP2DepositorInsertrsEGFP2
UseAAVTagsC-terminal (Ct) cytoplasmic domains of low densit…ExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR/eCas9-GPIDAF
Plasmid#213710PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.DepositorInsertEGFP
UseCRISPRTags3XFLAG and Twin-strep-tagExpressionMutationPromoterCMVAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR/eCas9-GPIBY55
Plasmid#213709PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.DepositorInsertEGFP
UseCRISPRTags3XFLAG and Twin-strep-tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
ePPEmax*
Plasmid#205244PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertc-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRTagsExpressionPlantMutationDetailed in manuscriptPromoterAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
IAA17-4flag-HIS5
Plasmid#207015PurposeFor ORF tagging with IAA17-4Flag. IAA17 is an auxin-inducible degron (AID).DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only