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Plasmid#80059PurposeMCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF
Plasmid#119864PurposeDonor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertBirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTagsHA-tagged biotin ligase (BirA), Thosea asigna vir…ExpressionBacterialMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-UtrCH
Plasmid#124656PurposeF-actin marker. EBFP2 is fused to the calponin homology domain of utrophin (UtrCH).DepositorInsertUtrophin (UTRN Human)
UseTagsEBFP2ExpressionMammalianMutationsee depositor comments belowPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV Cbx3-V5
Plasmid#175104PurposeLentiviral expression of mouse Cbx3-V5DepositorInsertCbx3 (Cbx3 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
Mouse GFP-PRMT7 LDIG(69-72)AAAA
Plasmid#65044PurposeExpresses Catalytically inactive GFP-PRMT7 LDIG(69-72)AAAA mutantDepositorInsertPRMT7 (Prmt7 Mouse)
UseBacterial propagationTagsGFPExpressionMammalianMutationL69A D70A I71A G72APromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHRIG-PRAS40
Plasmid#53599Purposelentiviral expression of constitutively active PRAS40 and EGFPDepositorInsertPRAS40 (AKT1S1 Human)
UseLentiviralTagsIRES-EGFP and mycExpressionMammalianMutationconstitutively active (myristoylated version)PromoterCMVAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
tdEos-Keratin-17
Plasmid#57629PurposeNote- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertKeratin (KRT18 Human)
UseTagstdEosExpressionMammalianMutationV282LPromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-Ef1a-FAS-NES-jRGECO1a-WPRE
Plasmid#141236PurposeAAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of jRGECO1a (jRGECO1a will not be expressed in mammalian cells that express Cre recombinase)DepositorInsertNES-jRGECO1a
UseAAV and Cre/Lox; Cre-offTags6xHIS tag and nuclear export signalExpressionMutationPromoterhuman elongation factor-1 alpha (EF-1 alpha)Available sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
B2M-SDMutation-gRNA
Plasmid#215547PurposegRNA targeting B2M to introduce splice donor mutation on the first intron of the locusDepositorInsertB2M (B2M Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFlare9A-Clip2E9 WT
Plasmid#209574Purposeminigene construct for Clip2 E9 alternative splicingDepositorInsertClip2 (Clip2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-deltaExon15-III
Plasmid#209567PurposeMammalian expression of Shtn1 long isoform with E15 deletion IIIDepositorInsertShtn1 long isoform with E15 deletion III (4930506M07Rik Mouse)
UseTagsExpressionMammalianMutationExon15 deletion IIIPromoterAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
INS-3'gRNA
Plasmid#210468PurposegRNA targeting INS 3' terminal for CRISPR-Cas9-mediated knock-inDepositorInsertINS (INS Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAdEasy-hALDOA D34S
Plasmid#210813PurposeTo reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)DepositorInsertALDOA (ALDOA Human)
UseAdenoviralTagsHAExpressionMutationresidues 34 of ALDOA mutated to serinePromoterCMVAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-hALDOA K230A
Plasmid#210811PurposeTo block the formation of the initial Schiff base with FBPDepositorInsertALDOA (ALDOA Human)
UseTagsHAExpressionMammalianMutationresidues 230 of ALDOA mutated to alaninePromoterCMVAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hALDOA D34S
Plasmid#210807PurposeTo reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)DepositorInsertALDOA (ALDOA Human)
UseLentiviralTagsHAExpressionMutationresidues 34 of ALDOA mutated to serinePromoterCMVAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGIG-FLAG-SHTN1LRRR>GGG
Plasmid#209570PurposeMammalian expression of Shtn1 long isoform with RG mutationDepositorInsertShtn1 long isoform with RG mutation (4930506M07Rik Mouse)
UseTagsExpressionMammalianMutationRRR to GGGPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free NES (M)
Plasmid#182437PurposeYeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS(M)
NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10 and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_YTHDF2_KO_Neo
Plasmid#186672PurposeDonor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. Neomycin and polyA sequence is inserted between two homology arms.DepositorInsertYTHDF2 KO Neomycin (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_YTHDF2_KO_Hygro
Plasmid#186671PurposeDonor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Hygromycin and polyA sequence is inserted between two homology arms.DepositorInsertYTHDF2 KO Hygromycin (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_YTHDF2_KO_Puro
Plasmid#186670PurposeDonor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms.DepositorInsertYTHDF2 KO Puromycin (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only