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Plasmid#47392DepositorInsertIntersectin short (ITSN1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJS699
Plasmid#168779PurposeEncodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombinationDepositorInsertSpike RBD (S betacoronavirus)
UseTagsCmycExpressionMutationN343QPromoterGAL1Available sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUDP012
Plasmid#101167PurposeE. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)DepositorInsertHH-gRNA-HDV targetting SeILV6 in S. pastorianus
UseCRISPRTagsExpressionYeastMutationPromoterScTDH3Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSin-5HT-1.0
Plasmid#172882Purposemake sindbis virus of 5-HT sensor GRAB_5-HT1.0 and express in cellsDepositorInsert5HT SENSOR
UseTagsExpressionMammalianMutationPromotersp6Available sinceOct. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS18
Plasmid#158787PurposeRibosome-tagged GCaMP (soma localized) expression pan-neuronally C. elegansDepositorInsertPtag-168::GCaMP6m::rpl-1a
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
DJ-1 TEV site pET15b
Plasmid#60687PurposeRecombinant protein expression of Park7DepositorInsertDJ-1 (PARK7 Human)
UseTags6xHisExpressionBacterialMutationTEV site was inserted (see comments below).PromoterT7Available sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-pegRNA_FANCF-EGFP
Plasmid#159785PurposeS. pyogenes pegRNA for C to A mutation at the FANCF site of human cells using prime editingDepositorInsertspacer of pegRNA targeting FANCF gene (FANCF Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-pegRNA_HOXD13_CtoT-EGFP
Plasmid#159792PurposeS. pyogenes pegRNA for C to T mutation at the HOXD13 site of mouse cells using prime editingDepositorInsertspacer of pegRNA_HOXD13_CtoT targeting HOXD13 gene (HOXD13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-pegRNA_HOXD13_CtoG-EGFP
Plasmid#159791PurposeS. pyogenes pegRNA for C to G mutation at the HOXD13 site of mouse cells using prime editingDepositorInsertspacer of pegRNA_HOXD13_CtoG targeting HOXD13 gene (HOXD13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS19
Plasmid#158788PurposeRibosome-tagged GCaMP (soma localized) expression in C. elegans ASH neuronsDepositorInsertpsra-6::GCaMP6m::rpl-1a
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS16
Plasmid#158786PurposeRibosome-tagged GCaMP (soma localized) expression in C. elegans AFD neurons (and others)DepositorInsertPntc-1::GCaMP6m::rpl-1a
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
M26I DJ-1 TEV site pET15b
Plasmid#60685PurposeRecombinant protein expression of Park7DepositorInsertM26I DJ-1 (PARK7 Human)
UseTags6xHisExpressionBacterialMutationM26I and TEV site was inserted (see comments belo…PromoterT7Available sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
M26L DJ-1 TEV site pET15b
Plasmid#60686PurposeRecombinant protein expression of Park7DepositorInsertM26L DJ-1 (PARK7 Human)
UseTags6xHisExpressionBacterialMutationM26L and TEV site was inserted (see comments belo…PromoterT7Available sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMA5-PSMA6-PSMA7
Plasmid#217894PurposeInsect cell expression of human 20S CP proteasome subunit alpha type-5 (PSMA5), alpha type-6 (PSMA6), and alpha type-7 (PSMA7)DepositorUseTagsExpressionInsectMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDON223 Spike D138Y
Plasmid#180056PurposeSars-CoV-2 protein mutationDepositorInsertSARS-CoV-2 Spike D138Y (S SARS-CoV-2)
UseTagsMAC-tagExpressionMammalianMutationSpike D138YPromoterAvailable sinceJan. 14, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDON223 Spike A570D
Plasmid#180064PurposeSars-CoV-2 protein mutationDepositorInsertSARS-CoV-2 Spike A570D (S SARS-CoV-2)
UseTagsMAC-tagExpressionMammalianMutationSpike A570DPromoterAvailable sinceJan. 14, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDON223 Spike H655Y
Plasmid#180059PurposeSars-CoV-2 protein mutationDepositorInsertSARS-CoV-2 Spike H655Y (S SARS-CoV-2)
UseTagsMAC-tagExpressionMammalianMutationSpike H655YPromoterAvailable sinceJan. 13, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDON223 Spike S982A
Plasmid#180067PurposeSars-CoV-2 protein mutationDepositorInsertSARS-CoV-2 Spike S982A (S SARS-CoV-2)
UseTagsMAC-tagExpressionMammalianMutationSpike S982APromoterAvailable sinceJan. 13, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDON223 Spike T1027I
Plasmid#180058PurposeSars-CoV-2 protein mutationDepositorInsertSARS-CoV-2 Spike T1027I (S SARS-CoV-2)
UseTagsMAC-tagExpressionMammalianMutationSpike T1027IPromoterAvailable sinceJan. 13, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDON223 Spike D1118H
Plasmid#180068PurposeSars-CoV-2 protein mutationDepositorInsertSARS-CoV-2 Spike D1118H (S SARS-CoV-2)
UseTagsMAC-tagExpressionMammalianMutationSpike D1118HPromoterAvailable sinceJan. 13, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits