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Showing: 5301 - 5320 of 64414 results

  3. pB80-hsKIF5B(1-560)Rigor-L-mVenus

    Plasmid
    #193720
    Purpose
    Expression of mVenus tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules
    Depositor
    Lukas Kapitein
    Article
    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:
    Insert
    hsKIF5B(1-560)Rigor-L-mVenus (KIF5B Synthetic, Human)
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    hsKIF5B(aa1-560), RIGOR: G234A; mVenus: Met1Del, …
    Promoter
    Chicken beta-actin
    Available Since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    193720-plasmid-map-sequence-id-383731

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  4. pFLAG-CMV2-Hsp27-S15D/S82D

    Plasmid
    #85186
    Purpose
    Hsp27 expression plasmid
    Depositor
    Ugo Moens
    Insert
    Hsp27 (HSPB1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    S15D/S82D, phosphomimicking mutation
    Promoter
    CMV
    Available Since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    85186-plasmid-map-sequence-id-167858

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  5. pFLAG-CMV2-Hsp27-S15D/S78D

    Plasmid
    #85185
    Purpose
    Hsp27 expression plasmid
    Depositor
    Ugo Moens
    Insert
    Hsp27 (HSPB1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    S15D/S78D, phosphomimicking mutation
    Promoter
    CMV
    Available Since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    85185-plasmid-map-sequence-id-167841

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  6. pFLAG-CMV2-Hsp27-S78A/S82A

    Plasmid
    #84996
    Purpose
    Hsp27 expression plasmid
    Depositor
    Ugo Moens
    Insert
    Hsp27 (HSPB1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Serine 78 and 82 mutated to Alanine
    Promoter
    CMV
    Available Since
    Dec. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    84996-plasmid-map-sequence-id-167724

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  7. pFLAG-CMV2-Hsp27-S15A/S82A

    Plasmid
    #84995
    Purpose
    Hsp27 expression plasmid
    Depositor
    Ugo Moens
    Insert
    Hsp27 (HSPB1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Serine 15 and 82 mutated to Alanine
    Promoter
    CMV
    Available Since
    Dec. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    84995-plasmid-map-sequence-id-167719

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  10. pNUT N6His Y95F/Y188F hTFNG

    Plasmid
    #69601
    Purpose
    Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the N-lobe
    Depositor
    Ross MacGillivray, Anne Mason
    Article
    Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2
    Insert
    mutated human serum transferrin (TF Human)
    Tags
    Hexa His tag and N-terminal signal peptide, 4 aa …
    Expression
    Mammalian
    Mutation
    Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe
    Promoter
    SV40
    Available Since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    69601-plasmid-map-sequence-id-124729

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  12. pVRC8400-SARS-CoV-2-RBD(aa330-526)-B.1.1.529-AVI

    Plasmid
    #182930
    Purpose
    Expresses SARS-CoV-2 RBD (aa324-526) domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-RBD(aa324-526)-B.1.1.529 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    G339D, S371L, S373P, S375F, K417N, N440K, G446S, …
    Available Since
    March 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    182930-plasmid-map-sequence-id-358143

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  13. PHPT1A

    Plasmid
    #42360
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PHPT1A (PHPT1 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. pSLPB2B-FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187951
    Purpose
    FKBP12 (F36V mutant) degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187951-plasmid-map-sequence-id-373504

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  17. Zhou-COVID-19-Spike

    Plasmid
    #161029
    Purpose
    Extracellular domain of SARS-CoV-2 spike protein that has been engineered so that it assembles pseudo virus more efficiently. Also contains portions of VSV-G for production of psuedovirus.
    Depositor
    Dapeng Zhou
    Article
    Tong et al Vaccines (Basel). 2021 Mar 22;9(3). pii:
    Insert
    Engineered COVID-19 Spike protein, VSV-G (S SAR-CoV-2, Vesicular stomatitis virus)
    Expression
    Mammalian
    Mutation
    Codon optimized
    Available Since
    Jan. 12, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161029-plasmid-map-sequence-id-320615

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Showing: 5301 - 5320 of 64414 results