Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 64,853 results for: %s

Clear keywords
Showing: 5301 - 5320 of 64853 results

  1. sfGFP-ER-5

    Plasmid
    #56483
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 485, Emission: 507
    Depositor
    Michael Davidson
    Insert
    ER (CALR Human)
    Tags
    sfGFP
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available Since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. N-Terminal Split Cas9 D10A Nickase with GyrA intein

    Plasmid
    #58695
    Purpose
    Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Nsplit Intein, HA Tag, and NLS
    Expression
    Mammalian
    Mutation
    D10A nickase converting mutation to SpCas9
    Promoter
    CBh
    Available Since
    Sept. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58695-plasmid-map-sequence-id-90694

    Enlarge

  7. pKT-mIDH1(R132H)-Myc-FLAG-IRES-Kat

    Plasmid
    #133779
    Purpose
    Expresses IDH1 with a R132H mutation and has Myc and FLAG tags. IDH1(R132H) also has a katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Insert
    Isocitrate dehydrogenase 1 (IDH1 Human)
    Tags
    Myc and FLAG
    Expression
    Mammalian
    Mutation
    Arginine is mutated to histidine at amino acid po…
    Available Since
    Oct. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    133779-plasmid-map-sequence-id-261114

    Enlarge

  8. pFLAG-CMV2-Hsp27-S78D/S82D

    Plasmid
    #85187
    Purpose
    Hsp27 expression plasmid
    Depositor
    Ugo Moens
    Insert
    Hsp27 (HSPB1 Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    S78D/S82D, phosphomimicking mutation
    Promoter
    CMV
    Available Since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    85187-plasmid-map-sequence-id-173286

    Enlarge

  12. pIND-HA-ADH

    Plasmid
    #11991
    Depositor
    Steven Johnson
    Insert
    aldehyde dehydrogenase (ALDH1A1 Human)
    Use
    Ecdysone inducible
    Tags
    HA
    Expression
    Mammalian
    Mutation
    no mutations
    Available Since
    July 25, 2007
    Availability
    Academic Institutions and Nonprofits only

  16. AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)_FS

    Plasmid
    #191527
    Purpose
    Donor for targeted integration of 3xFLAG-2xSTREP tagged S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    hGEM(1-110)_mNG_hSLBP(18-108)
    Use
    CRISPR and Synthetic Biology
    Tags
    mNeonGreen (mNG) + 3xFLAG_TwinStrep
    Expression
    Mammalian
    Available Since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191527-plasmid-map-sequence-id-378107

    Enlarge

  17. pVRC8400-SARS-CoV-2-NTD-B.1.1.529-AVI

    Plasmid
    #181698
    Purpose
    Expresses SARS-CoV-2 NTD domain from the B.1.1.529 (Omicron) variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-NTD-B.1.1.529 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    A67V, ∆H69, ∆V70, T95I, G142D, ∆V143, ∆Y144, ∆Y14…
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    181698-plasmid-map-sequence-id-354496

    Enlarge

  18. pVRC8400-SARS-CoV-2-S2P-B.1.1.7-AVI

    Plasmid
    #176325
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.1.7 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.1.7 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    69-70 del, 144del, N501Y, A570D, D614G, P681H, T7…
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176325-plasmid-map-sequence-id-347062

    Enlarge

Showing: 5301 - 5320 of 64853 results