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Plasmid#83953PurposeFRET acceptor for TrkB mEGFPDepositorInsertPhospholipase C gamma 1 (Plcg1 Mouse)
UseTagsmRFPExpressionMammalianMutationPromoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mRuby4(1-10)
Plasmid#165143PurposeExpresses mRuby4(1-10) in mammalian cellsDepositorInsertmRuby4(1-10)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGPD2
Plasmid#43972DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterGPDAvailable sinceApril 9, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3/RGS-UBC12
Plasmid#20080DepositorInsertUBC12 (UBE2M Human)
UseTags6xHisExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/FGFR2myc
Plasmid#33248DepositorInsertFGFR2 (Fgfr2 Mouse)
UseTagsHis and MycExpressionMammalianMutationKpnI site introduced at stop codon of FGFR2 to be…PromoterCMVAvailable sinceDec. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXO1A
Plasmid#153141PurposeExpression of Flag tagged human FOXO1A in mammalian cellsDepositorInserthuman FOXO1 (FOXO1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF2990
Plasmid#144466PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNFE2L1 (NFE2L1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDD120
Plasmid#119879PurposeConstitutive expression of Che9c60 and Che9c61 recombinases on pB264/pBR322 backbone with kanamycin resistance.DepositorInsertpConstitutive_Che9c60 and Che9c61
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 NL
Plasmid#113442PurposeNanoLuc control expression vectorDepositorInsertNanoLuc
UseLuciferaseTagscmycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWMD23
Plasmid#140006PurposeExpression of ORF1 (Shuffle 1 NLS) and Pong TPase (L418A, L420A)DepositorInsertsORF1 Shuffle 1 NLS
Pong TPase LA
UseTagsExpressionPlantMutationNES sequence mutated L418A, L420A and Ping N-term…PromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRiA4-VIR
Plasmid#138193PurposeVirulence helper compatible with pCambia binary vectors.DepositorInsertvir gene cluster
UseVir gene expressionTagsExpressionMutationPromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.iGluSnFR3.v857.PDGFR
Plasmid#178329PurposeFluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857DepositorHas ServiceAAV1InsertpAAV hSyn iGluSnFR3 v857.PDGFR
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and PDGFR TM DomainExpressionMammalianMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHyo245
Plasmid#173147PurposeExpresses pheS_A294G by dual T7 promoter in e.coliDepositorInsertpheS_A294G
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEH_AAV_TERT_prom_-146C>T_C7C_1.8kb
Plasmid#183081PurposerAAV transfer plasmid with ITRs flanking: (1) TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.DepositorInsertTERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
UseAAV; Homologous recombination donor templateTagsExpressionMutation-146C>T promoter, Exon 1 C7C (C21>T21) sile…PromoterNone for primary insert (Though partial TERT prom…Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-M2
Plasmid#135583PurposeHigh eukaryotic protein expression shuttle vectorDepositorTypeEmpty backboneUseTransfer vectorTagsExpressionInsectMutationPromoterpolyhedrin of AcMNPVAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV PIG (Puro IRES GFP empty vector)
Plasmid#21654PurposeRetroviral backbone for cloning and gene expression. Select with puromycin or GFP.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsGFP and IRESExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr-AcGFP1-ORP9-PH
Plasmid#214270PurposeMammalian expression of green fluorescent PI4P reporter AcGFP1-ORP9-PHDepositorInsertAcGFP1-ORP9(1-111) (Osbpl9 Mouse)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-mGFP-FMR1 isoform1-sh1Resistant
Plasmid#225462PurposeExpress mEGFP-fusion protein of FMR1 isoform 1DepositorInsertFMR1 isoform 1 (FMR1 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUltra-hot
Plasmid#24130Purpose3rd generation Lentiviral vector for bi-cistronic expression of mCherry and the gene of interestDepositorTypeEmpty backboneUseLentiviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMK393 (mAID-mCherry-BSD)
Plasmid#121194PurposeC-terminal taggingDepositorInsertmAID-mCherry-BSD
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FlipCherry-T2A-GFP
Plasmid#124436PurposeExpresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cellsDepositorInsertFlipCherry-T2A-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.NES-jRGECO1b.WPRE.SV40
Plasmid#100857PurposeAAV expression of jRGECO1b, a red fluorescent calcium sensor protein, from the Synapsin promoterDepositorHas ServiceAAV1InsertjRGECO1b
UseAAVTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-Daxx/pRK5
Plasmid#52023PurposeExpresses HA-Daxx in mammalian cellsDepositorInsertHA-Daxx (DAXX Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRK5-LAMP1-FLAG
Plasmid#71868PurposeoverexpressionDepositorInsertLAMP1 (LAMP1 Human)
UseTagsFLAGExpressionMammalianMutationPromotercmvAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTS2395-Tier1-PhCMV-Igk_mRuby2_KDEL
Plasmid#169533PurposeTier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 localized to the ER (PhCMV-Igk-mRuby2-KDEL-pA).DepositorInsertPCMV-driven ER-localized monomeric red fluorescent protein mRuby2
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR GFP-CaaX
Plasmid#113020Purposefor lentivirus production of GFP-CaaXDepositorInsertGFP-CaaX
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV500 A-CREB
Plasmid#33371DepositorInsertCREB (Creb1 Mouse)
UseTagsFLAGExpressionMammalianMutationDominant Negative (see notes)PromoterCMVAvailable sinceDec. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC3A2_STOP
Plasmid#161379PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC3A2 (SLC3A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
TFORF0918
Plasmid#142199PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNCOA1 (NCOA1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
4xmts-mScarlet-I
Plasmid#98818PurposeIn vivo visualization of mitochondria (can be used for colocalization studies)DepositorArticleInsertmts
UseTagsmScarlet-IExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
XRE-pNL1.3[secNLuc]
Plasmid#182295PurposeSecreted Luciferase reporter for AhR activityDepositorInsertXenobiotic Response Element (CYP1A1 Human)
UseTagssecNlucExpressionMammalianMutationPromoterXREAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
Plasmid#20298PurposeCre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activationDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9Insertchannelrhodopsin-2
UseAAVTagsEYFPExpressionMammalianMutationhumanized ChR2 gene with histidine 134 changed to…PromoterEF1alphaAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNL4-3 envFS eGFP Δvpu/env
Plasmid#101345PurposeDeletion from NL4-3 nt 6054-7489 encompassing all of vpu and env until before the RREDepositorInsertNL4-3
UseLentiviralTagsExpressionMutationdelta vpu/EnvPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBV-Luc wt MBS1-4
Plasmid#16564DepositorInsertc-MYC binding sites (MYC Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pB-TopFlash-NanoLucP
Plasmid#204347PurposeTopFlash reporterDepositorInsertTopFlash-NanoLucP
UseLuciferaseTagsNanoLucPExpressionMutationPromoterminiPAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST-mCherry-ARHGEF2
Plasmid#207959PurposeExpression vector for ARHGEF2 (GEF-H1) with an N-terminal mCherry tagDepositorInsertARHGEF2 (ARHGEF2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI-SEP GluR2(R)
Plasmid#24001DepositorInsertGluR2 (Gria2 Rat)
UseTagsGluR1 signal peptide (aa1-22) and SEP (supereclip…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
TFORF2885
Plasmid#144256PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGLI2 (GLI2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
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mCherry-Sec23A
Plasmid#166894Purposemammalian expression of Sec23A tagged with mCherryDepositorInsertSEC23A (SEC23A Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only