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Plasmid#74369PurposeThis plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.DepositorArticleInsertRGR-r11
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAHD1Available sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSEM232 - [Pmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR]
Plasmid#159898PurposeFluorescent co-injection marker to identify extrachromosomal arrays in C. elegansDepositorInsertPmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
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p7128 pHAGE-P-CMVt-N-HA-GAW-HLA-A
Plasmid#100154PurposeExpresses HLA-ADepositorInsertHLA-A (HLA-A Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTEI036
Plasmid#105350PurposeSynthetic biologyDepositorInsertLexA operator & 35S min promoter
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNAS1B split mCherry N-link/link-C
Plasmid#61965PurposeNegative control duet plasmid for split mCherry assembly assay (i.e., neither half of mCherry is fused to another peptide or protein)DepositorInsertsN-mCherry-link
link-C-mCherry
UseTagsExpressionBacterialMutationPromoterPBAD and PLtetOAvailable sinceFeb. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAGM1761
Plasmid#105381PurposeSynthetic biologyDepositorInsertpromoter AtDMC1 (At3G22880; 3000bp)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C
Plasmid#61966PurposePositive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))DepositorInsertsN-mCherry-ME(pS)VD
TRAP(RK)-C-mCherry
UseTagsExpressionBacterialMutationPromoterPBAD and PLtetOAvailable sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIK198
Plasmid#65629Purpose"flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.DepositorTypeEmpty backboneUseCRISPRTagsExpressionWormMutationPromoterU6Available sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPIC-mouseKir3.2-mC-S
Plasmid#140745PurposeExpression of mouse Kir3.2 fused to mCherry and Streptag IIDepositorInsertKir3.2 (Kcnj6 Mouse)
UseTagsmCherry with Streptag IIExpressionYeastMutationPromoterAOX1Available sinceMay 14, 2020AvailabilityAcademic Institutions and Nonprofits only