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pX459-sgAAVS1

Plasmid

#184403

Purpose

pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

AAVS1-specific sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pSQT1313

Plasmid

#53370

Purpose

multiplex gRNA expression plasmid

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

None

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 19, 2014

Availability

Academic Institutions and Nonprofits only

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HA-CYLD-WT

Plasmid

#15506

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

CYLD (CYLD Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2007

Availability

Academic Institutions and Nonprofits only

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pIM1773-PRHIII-sfGFP-pNW33N

Plasmid

#52217

Purpose

Constitutive sfGFP expression plasmid for use in Geobacillus and E. coli

Depositor

Ichiro Matsumura

Article

Blanchard et al Appl Microbiol Biotechnol. 2014 May 2.

Insert

PRHIII-sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PRHIII

Available since

May 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas13-M3nls

Plasmid

#155366

Purpose

Targeted m6A RNA methylation in mammalian cells

Depositor

David Liu

Article

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Insert

dCas13-M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

PspCas13b Δ984-1090 H133A

Promoter

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR wt-FLAG

Plasmid

#204525

Purpose

Retroviral expression of human CALR wt-FLAG

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

CALR wt-FLAG (CALR Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti6.3/TO/V5-DEST-IGFBP2

Plasmid

#191006

Purpose

Lentiviral expression vector for IGFBP2

Depositor

Johanna Ivaska

Article

Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

Insert

Insulin-Like Growth Factor Binding Protein 2 (IGFBP2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ10873

Plasmid

#183961

Purpose

U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Inserts

HyperdCas12a
poly-crRNA array targeting Sox2-Klf4-Oct4

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D832A, D156R, E292R, D235R, D350R

Promoter

CAG and U6

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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SB100X in pCAG globin pA

Plasmid

#127909

Purpose

Sleeping Beauty Transposase expression vector driven by the CAG promoter

Depositor

Mark Groudine

Article

Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

Insert

Sleeping Beauty Transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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Beta-Globin-WT

Plasmid

#130700

Purpose

Expresses Beta-Globin WT NMD reporter

Depositor

Marvin Tanenbaum

Article

Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

Insert

Beta Globin (HBB Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Pcmv

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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