Showing: 5281 - 5300 of 16015 results
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Plasmid#186667PurposeDonor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, puromycin and polyA sequence is inserted between two homology arms.DepositorInsertANKRD1 KO Puromycin (ANKRD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Donor_ANKRD1_KO_Hygro
Plasmid#186668PurposeDonor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, hygromycin and polyA sequence is inserted between two homology arms.DepositorInsertANKRD1 KO Hygromycin (ANKRD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Cx26
Plasmid#69021PurposeExpresses rat Cx26 (Gjb2 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.DepositorUseTagsEBFP2ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pVH003
Plasmid#80397PurposeContains pBAD-CusR (response regulator) and pCusR-antiscaffold-YFP-AAV. This plasmid was used for microscopy tracking experimentsDepositorUseSynthetic BiologyTagsFused by GS linker to LZx domain and Venus has N-…ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/SH/GW-Mst1
Plasmid#84299PurposeExpresses a tetracycline-inducible SH-tagged (streptavidin-binding peptide and HA tag) version of Mst1. Used in conjunction with Flp-In cells to generate single copy transgene.DepositorInsertMst1 (STK4 Human)
UseTagsHA, SBPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSA1
Plasmid#52261PurposeBacterial expression of human 6His-tagged SUMO1 and SUMO-E1DepositorUseTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-SMO-1(GG)
Plasmid#87054PurposeExpresses 6xHis-tagged smo-1(GG), with a TEV protease cleavage site between the tag and the ORFDepositorInsertsmo-1 (smo-1 Nematode)
UseTags6xHis-TEVExpressionBacterialMutationThe last aa was removed to render processed SMO-1…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Venus-173-N-ORAI1
Plasmid#87618PurposeN-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.DepositorInsertVenus173-N-ORAI1 (ORAI1 Human, Synthetic)
UseTags6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AMPKsub-YPet-Kras
Plasmid#84638PurposeEncodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertAMPKsub-YPet-Kras
UseTags6xHis, C-terminal targeting sequence from Kras, T…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA SF-HERC2 F1
Plasmid#55784PurposeExpresses N-terminal 3xFLAG/STREP tagged HERC2 (1-969aa)DepositorInsert3xFLAG tagged HERC2 (1-969aa) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA SF-HERC2 F2
Plasmid#55785PurposeExpresses N-terminal 3xFLAG/STREP tagged HERC2 (950-1750aa)DepositorInsert3xFLAG tagged HERC2 (950aa-1750aa) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA SF-HERC2 F3
Plasmid#55786PurposeExpresses N-terminal 3xFLAG/STREP tagged HERC2 (1700-2700aa)DepositorInsert3xFLAG tagged HERC2 (1700aa-2700aa) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA SF-HERC2 F4
Plasmid#55787PurposeExpresses N-terminal 3xFLAG/STREP tagged HERC2(2600-3600aa)DepositorInsert3xFLAG tagged HERC2 (2600-3600aa) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA SF-HERC2 F5
Plasmid#55788PurposeExpresses N-terminal 3xFLAG/STREP tagged HERC2 (3550-4450aa)DepositorInsert3xFLAG tagged HERC2 (3550-4450aa) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA SF-HERC2 F6
Plasmid#55789PurposeExpresses N-terminal 3xFLAG/STREP tagged HERC2 (4421-4834aa)DepositorInsert3xFLAG tagged HERC2 (4421-4834aa) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-N-SH2
Plasmid#111274Purposeexpress murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
UseTagsHHHHHHENLYFQGExpressionBacterialMutationPromoterT7 promoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RAB-EKARev-NLS
Plasmid#118476PurposeddRFP-based single-color ERK biosensor targeted to the nucleus.DepositorInsertRAB-EKARev-NLS
UseTags6xHIS, Nuclear localization signal (NLS), T7 tag …ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgL-FLAG (TB40/E)
Plasmid#136449PurposeMammalian expression plasmid for HCMV glycoprotein L (TB40/E strain) with FLAG tagDepositorInsertglycoprotein L (UL115 Human betaherpesvirus 5)
UseTagsShort SGGSG linker and FLAG tagExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCEP4-gp120(BaL)-CD4
Plasmid#98957PurposeMammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolateDepositorInsertHIV-1 (BaL) gp120
UseTagsCD4 (domains D1-D2), CD5 leader sequence, FLAG ep…ExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
mCherry-CRY2clust
Plasmid#105624PurposeExpresses improved CRY2PHR homo-oligomerizationDepositorInsertmCherry-CRY2PHR-9aa (CRY2 Human, Mustard Weed)
UseTags9 amino acidsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 5281 - 5300 of 16015 results