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Plasmid#104118PurposesynOptopatch constructDepositorInsertCheRiff-CFP
UseLentiviralTagsExpressionMammalianMutationPromoterhuman synapsin IAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PCNA_PCNA
Plasmid#110022PurposeProtein expression and purification of PCNA_PCNADepositorInsertPCNA_PCNA (PCNA Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-T2A-GAL4-DBD
Plasmid#138394PurposeRMCE vector for generating C-terminal T2A-GAL4-DBD fusion (split GAL4)DepositorInsertT2A-GAL4-DBD
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_WT-talin1 (1-2541)
Plasmid#166112PurposeFor expression of full-length wild-type mouse talin-1 in mammalian cells. Contains C-terminal EGFP tag.DepositorInsertWT-Talin-1 (Tln1 Mouse)
UseCmv promoterTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1(+)-sapphireAKAR
Plasmid#118464Purposecp-T-sapphire-based single-color biosensor for monitoring Protein Kinase A activity.DepositorInsertsapphireAKAR
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+eGFP-bromo1
Plasmid#157805PurposeExpression of synthetic protein composed of eGFP and BRD4 bromodomain #1DepositorInserteGFP-bromo1
UseTagsHis6 and MBPExpressionBacterialMutationPromoterpTACAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
mc2 mCherry
Plasmid#206219PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAtCHYb1Δ129N
Plasmid#53367PurposeContains a truncated Arabidopsis beta-carotene hydroxylase cDNA, the product of which converts beta-carotene primarily to monocyclic beta-cryptoxanthin in E. coli. Use with pAC-BETA or pAC-BETAipi.DepositorInsertchyB1 (BETA-OHASE 1 Mustard Weed)
UseTagsExpressionBacterialMutationDeleted codons for the first 129 amino acidsPromoterTrcAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV)
Plasmid#140160PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMS79 (eft-3p::Cas9 + sgRNA)
Plasmid#154839PurposeCas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGGDepositorInsertsgRNA
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0108
Plasmid#91001PurposeModule A, Promoter: EC1.2, Gene: AtCas9, Terminator: HSPDepositorInsertAtCas9
UseCRISPRTagsExpressionMutationPromoterEC1.2Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV6M-Pak1 H83L H86L
Plasmid#12211DepositorInsertPak1 (PAK1 Human)
UseTagsMycExpressionMammalianMutationPBD (p21-binding domain) mutant: H83L, H86L (see …PromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+eGFP-bromo5
Plasmid#157804PurposeExpression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1DepositorInserteGFP-bromo5
UseTagsHis6 and MBPExpressionBacterialMutationPromoterpTACAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp2-GFPuv
Plasmid#139083PurposeLow phosphate inducible promoter yibDp2 for GFP expressionDepositorInsertyibDp2-GFPuv
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTC223
Plasmid#70019PurposeCas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7DepositorInsertNuclease (Cas9/sgRNA) + Donor + GVR
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pME-APEX2-NS
Plasmid#67669PurposeGateway entry clone containing APEX2 with no stop codonDepositorInsertAPEX2
UseGateway multisite middle entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
LZF42: Cre-off hSyn-QuasAr3-Citrine (FAS)
Plasmid#104116PurposesynOptopatch constructDepositorInsertQuasAr3-Citrine
UseLentiviralTagsExpressionMammalianMutationPromoterhuman synapsin IAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
px459 sgFIP200
Plasmid#175024PurposeCRISPR-Cas9 plasmid targeting exon 3 of human FIP200.DepositorInsertRB1CC1 (RB1CC1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
MTK234_032
Plasmid#123995PurposeEncodes the spCas9 sgRNA1 hAAVS1 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA1 -hAAVS1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only