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EF2451_pET21a(+)

Plasmid

#13840

Depositor

SeWon Suh

Article

Kang et al Acta Crystallograph Sect F Struct Biol C

Insert

PPAT

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

June 29, 2007

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_BsmBI_Intein_DD

Plasmid

#162810

Purpose

STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

BsmBI_Intein_DD

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*GFP

Plasmid

#162806

Purpose

STELLA plasmid for amber suppression of N-terminally labelled GFP

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

EF1_Ub-(N-TAG)GFP

Use

Piggybac

Tags

Expression

Mammalian

Mutation

GFP (Met1,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pARiBo1

Plasmid

#98892

Purpose

Expresses ARiBo-fused RNA in vitro with T7 RNA polymerase

Depositor

Pascale Legault

Article

Di Tomasso et al Nucleic Acids Res. 2011 Feb;39(3):e18. d

Insert

ARiBo1

Use

In vitro transcription vector

Tags

Expression

Mutation

Promoter

T7 promoter

Available since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSec7-iGFPx6

Plasmid

#115424

Purpose

Express Sec7-iGFPx6 at the endogenous locus via pop-in pop-out mutagenesis

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

Sec7-iGFPx6

Use

Tags

iGFP

Expression

Yeast

Mutation

Promoter

Endogenous

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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LI C-D 11GFP

Plasmid

#54431

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

superfolder GFP 11 domain

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only

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hSDH5_G78R

Plasmid

#113047

Purpose

Expression of mutant hSDH5

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

hSDH5_G78R (SDHAF2 Human)

Use

Tags

Expression

Bacterial

Mutation

G78R, Insert begins at amino acid 39

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B8-TEF(1004)

Plasmid

#44366

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(1004) promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(1004)

Available since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pDHJS3 AN-

Plasmid

#78241

Purpose

One of four plasmids needed to create the mismatched double Holliday junction substrate (MM-DHJS).

Depositor

Tao-shih Hsieh

Article

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "A" with NheI site
loxP2

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B6-Leum

Plasmid

#44314

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B6-Leum promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B6-Leum

Available since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pDHJS3 AS-

Plasmid

#78237

Purpose

One of four plasmids needed to create the double Holliday junction substrate (DHJS).

Depositor

Tao-shih Hsieh

Article

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "B"
loxP2

Use

Phagemid

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Human NCKX4-AS-short (pcDNA3.1+)

Plasmid

#75210

Purpose

Mammalian expression of SLC24A4

Depositor

Jonathan Lytton

Article

Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

Insert

SLC24A4 (SLC24A4 Human)

Use

Tags

Expression

Mammalian

Mutation

alternatively spliced exon absent

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pGST1-yGGA1-VHS

Plasmid

#44421

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

yGGA1-VHS (GGA1 Budding Yeast)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pUC-UAS1B12-Leum

Plasmid

#44320

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B12-Leum promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B12-Leum

Available since

April 17, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B5-Leum

Plasmid

#44313

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B5-Leum promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B5-Leum

Available since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B10-Leum

Plasmid

#44318

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B10-Leum promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B10-Leum

Available since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B8-TEF(203)

Plasmid

#44378

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(203) promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(203)

Available since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B8-TEF(804)

Plasmid

#44368

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(804) promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(804)

Available since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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AICSDP-167:CDH5-mEGFP

Plasmid

#217425

Purpose

Homology arms and mEGFP sequence for C-terminus tagging of human cadherin 5

Depositor

Allen Institute for Cell Science

Insert

CDH5 Homology Arms with mEGFP (CDH5 Human)

Use

CRISPR; Donor template

Tags

mEGFP

Expression

Mutation

homology arms contain point mutations to disrupt …

Promoter

Available since

Nov. 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJBM1

Plasmid

#225288

Purpose

E. coli- C. coli shuttle vector; KanR

Depositor

Robert Moore

Article

McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

Insert

Replication elements from pWestmill 11

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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