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pM2P20X
Plasmid#108210PurposeExpresses MSePCNA2-PdX fusion protein in E. coliDepositorInsertMsePCNA1-PdX fusion protein
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pS3C
Plasmid#108211PurposeExpresses SsoPCNA3-CYP101A1 fusion protein in E. coliDepositorInsertSsoPCNA3-CYP101A1 fusion protein
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pM3C
Plasmid#108212PurposeExpresses MsePCNA3-CYP101A1 fusion protein in E. coliDepositorInsertMsePCNA3-CYP101A1 fusion protein
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pM1R
Plasmid#108208PurposeExpresses MsePCNA1-PdR fusion protein in E. coliDepositorInsertMsePCNA1-PdR fusion protein
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pS1R
Plasmid#108207PurposeExpresses SsoPCNA1-PdR fusion protein in E. coliDepositorInsertSsoPCNA1-PdR fusion protein
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pISH-Vmn1r105
Plasmid#105994PurposeTo prepare riboprobe for in situ hybridizationDepositorUseTo generate riboprobeTagsExpressionMutationPromoterAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAS2-1-Brr2
Plasmid#99995PurposeExpress yeast Brr2 with Y2H binding domain fusionDepositorInsertBrr2 (BRR2 Budding Yeast)
UseTagsY2H binding domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACT2-Rcl1
Plasmid#99992PurposeExpress yeast Rcl1 with Y2H activation domain fusionDepositorInsertRcl1 (RCL1 Budding Yeast)
UseTagsY2H activation domainExpressionYeastMutationContains amino acids 1-217 of Rcl1PromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACT2-Kre33
Plasmid#99990PurposeExpress yeast Kre33 with Y2H activation domain fusionDepositorInsertKre33 (KRE33 Budding Yeast)
UseTagsY2H activation domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS2-1-Kre33
Plasmid#99989PurposeExpress yeast Kre33 with Y2H binding domain fusionDepositorInsertKre33 (KRE33 Budding Yeast)
UseTagsY2H binding domainExpressionYeastMutationPromoterAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
CC1-Pos6
Plasmid#101656PurposeCC1-Pos6 is a modified version of CC1-M240 that has 4 arginines in its linker. (* super ecliptic pHluorin A227D)DepositorInsertCC1-Pos6
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
CC1-M240
Plasmid#101657PurposeCC1 with shorter linker length and the 'GDP' fusion site. (* super ecliptic pHluorin A227D)DepositorInsertCC1-M240
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCML 220
Plasmid#98588PurposepCYFP. Expresses CYFP control fusion in E. coli for TriFC assay (kanR).DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterpLacOAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
LPhP-pHG165
Plasmid#90047PurposeConstitutive expression of a chimeric LacI:PurR transcription repressorDepositorInsertLPhP
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pET21a_Msm_RbpA
Plasmid#87928PurposeMycobacterium smegmatis RpbA in pET21a (ampR) used to co-express with Msm SigA for crystallization purposesDepositorInsertRbpA
UseTagsExpressionBacterialMutationPromoterT7PAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1852-5 pHR: U6-SpsgCD95-3 CMV-EGFP
Plasmid#84253PurposeExpresses Sp sgCD95-3 gRNA with an EGFP fluorescent markerDepositorInsertSp sgCD95-3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6Available sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1852-3 pHR: U6-SpsgCD95-2 CMV-EGFP
Plasmid#84252PurposeExpresses Sp sgCD95-2 gRNA with an EGFP fluorescent markerDepositorInsertSp sgCD95-2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6Available sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHU6_Ch5_155183064-gRNA-for
Plasmid#81214PurposepHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 3' region of pCALNL_Ch5 reporterDepositorInserthU6 expression of gRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only
