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Plasmid#143831PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertARID5B (ARID5B Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-Membrin-IRES-Puromycin
Plasmid#134865PurposeExpresses EGFP-tagged Golgi apparatus markerDepositorInsertMembrin (GOSR2 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgSmarca4#1/Cre
Plasmid#173619PurposeExpresses a Smarca4-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Smarca4 (Smarca4 Mouse)
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgSmarca4#2/Cre
Plasmid#173620PurposeExpresses a Smarca4-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Smarca4 (Smarca4 Mouse)
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid
Plasmid#154429Purposehu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmidDepositorInserthu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid
UseCRISPR and LentiviralTagsExpressionMammalianMutationVRQR point mutations in SpCas9PromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF0976
Plasmid#142223PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGFI1B (GFI1B Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-ESR1-Y537N
Plasmid#116373PurposeLentiviral expression of ESR1 Y537NDepositorInsertESR1 (ESR1 Human)
UseLentiviralTagsExpressionMutationY537NPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pLVX-EF1alpha-nCoV2019-N-meGFP-IRES-Puro
Plasmid#185451PurposeExpresses SARS-CoV-2 nucleocapsid protein with a c-terminal monomeric EGFP in mammalian cellsDepositorInsertNucleocapsid (N SARS-CoV-2)
UseLentiviralTagsmEGFPExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSC101 NL4-3 envFS Δrev ΔRRE RTEm26CTE
Plasmid#101351PurposeHIV-1 delta Rev was cloned into pSC101 and modified to include an RTEm26CTE element in order to utilize the NXF1 RNA export pathway. The RRE was also mutated.DepositorInsertNL4-3
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
plKO.1-DNAJC5-ShRNA
Plasmid#205729PurposeKnockdown of DNAJC5DepositorInsertshRNA targeting DNAJC5 (DNAJC5 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-DNAJC5 (L115R)
Plasmid#205728PurposeMammalian expression of DNAJC5 (L115R)DepositorInsertDNAJC5 (L115R) (DNAJC5 Human)
UseLentiviralTagsExpressionMutationL115RPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2242
Plasmid#141986PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertGATA1 (GATA1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-dCas9-VP64-MS2-VP64-Blast
Plasmid#192666Purpose3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A Blast resistance markerDepositorInsertdCas9-VP64, MS2-VP64
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A and N863A in Cas9PromoterEF1aAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX208_CMV::mCherry-myc-VAMP2
Plasmid#199716PurposeExpresses VAMP2, fused to mCherry, in mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.DepositorInsertmCherry-myc-VAMP2
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HF1RA-P2A-GFP-PGK-Puro
Plasmid#110866PurposeLentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)DepositorInsertCas9-HF1RA
UseLentiviralTagsFLAGExpressionMutationR661A, Q695A, Q926A, and NLS sequence at the N-te…PromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-SETDB1 ts1
Plasmid#115859PurposeSETDB1 knockdownDepositorInsertSETDB1 shRNA (SETDB1 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Rictor-Myc
Plasmid#157931PurposeOver expression of RictorDepositorInsertRictor (Rictor Mouse)
UseLentiviralTagsHis and MycExpressionMammalianMutationPromoterUbiquitinc PromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
SHP2(WT)@pLKO-Trex-SBP
Plasmid#85457PurposeGene expressionDepositorInsertSHP2 (PTPN11 Human)
UseLentiviralTagsSBP TagExpressionMammalianMutationPromoterRSVAvailable sinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJA291
Plasmid#74487PurposeeF1alpha::puroR::mCherry::T2A::eGFP::MCS::wPRE for assaying effects of C-terminal fusions to eGFPDepositorInsertpuroR-mCherry-T2A-eGFP
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only