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Plasmid#186536PurposeLocalization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to mitochondriaDepositorInsertLSSmGFP
UseTagsHuman COX8a first 29 amino acidsExpressionMammalianMutationhfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206KPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQHA-USP7 WT puroR
Plasmid#46753PurposeRetroviral vector that expresses wild type HA-tagged human USP7DepositorInsertUSP7 (USP7 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDD283
Plasmid#66824PurposeYPET^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertYPET-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized YPETExpressionWormMutationPromoterAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
ER2-LSSmGFP
Plasmid#186538PurposeLocalization and retention of LSSmGFP fluorescent protein (ex/em = 400/510 nm) in the ER; improved targeting efficiencyDepositorInsertLSSmGFP
UseTagsCalreticulin and KDELExpressionMammalianMutationhfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206KPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
ER2-mGreenLantern
Plasmid#186548PurposeLocalization and retention of mGreenLantern fluorescent protein in the ER; improved targeting efficiencyDepositorInsertmGreenLantern
UseTagsCalreticulin and KDELExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-3xhfYFP
Plasmid#186544PurposeHyperfolder YFP, triple fusion: cytosolic expression in mammalian cellsDepositorInsertHyperfolder YFP (3X)
UseTagsExpressionMammalianMutationmGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…PromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV Flag cycT2b (P#1718)
Plasmid#14599DepositorInsertcycT2 (CCNT2 Human)
UseTagsFlagExpressionMammalianMutationA137T compared to NP_490595.1PromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBAD-FOLD6
Plasmid#186547PurposeFOLD6 "hyperfolder" green fluorescent protein: bacterial expression plasmidDepositorInsertFOLD6
UseTags6xHisExpressionBacterialMutationmGreenLantern-C48S/V68L/C70V/K101E/T105Y/S147P/K1…PromoteraraBAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pECFP-Cdc42Q61L
Plasmid#105294PurposeConstitutively active Cdc42DepositorInsertCdc42 (CDC42 Human)
UseTagsCFPExpressionMammalianMutationQ61LPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tol2-cmlc2-Trpa1b-2A-EGFP
Plasmid#106405PurposeFor expressing Trpa1b-2A-EGFP in zebrafish cardiomyocytes.DepositorInsertsUseGatewayTags2A-EGFPExpressionMutationPromoterAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tol2-ngn1-Trpa1b-2A-mCherry
Plasmid#106425PurposeFor expressing Trpa1b-2A-mCherry in zebrafish Rohon-Beard neurons.DepositorInsertsUseGatewayTags2A-mCherryExpressionMutationPromoterAvailable sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-LSSmGFP
Plasmid#186540PurposeVisualization of the ER in cells using LSSmGFP (ex/em = 400/510 nm); compatible with OSER assay.DepositorInsertLSSmGFP
UseTagsCytochrome p450 first 29 animo acids (Oryctolagus…ExpressionMammalianMutationhfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206KPromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPO635
Plasmid#195297PurposepACYC-plac-MycaTnsABC, lac promoter expressing transposase genes TnsABC of McCAST (Tn7575).DepositorInsertTnsABC operon of McCAST (Tn7575)
UseTagsExpressionBacterialMutationPromoterIPTG inducible lac promoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
SRS-MTBD(22/19), AB(monomer), wt
Plasmid#22380DepositorInsertSeryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
UseTagsHis and mycExpressionBacterialMutationPromoterAvailable sinceNov. 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
ER2-hfYFP
Plasmid#186518PurposeLocalization and retention of hyperfolder YFP fluorescent protein in the ER; improved targeting efficiencyDepositorInsertHyperfolder YFP
UseTagsCalreticulin and KDELExpressionMammalianMutationmGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…PromoterCMVAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME-Trpa1b-2A-mCherry
Plasmid#106291PurposeGateway middle entry construct containing zebrafish Trpa1b, 2A self-cleaving peptide and mCherryDepositorInsertTrpa1b (trpa1b Zebrafish)
UseGateway middle entryTags2A-mCherryExpressionMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPO636
Plasmid#195299PurposepACYC-plac-MycaTnsABCQ, lac promoter expressing transposase genes TnsABCQ of McCAST (Tn7575).DepositorInsertTnsABCQ operon of McCAST (Tn7575)
UseTagsExpressionBacterialMutationPromoterIPTG inducible lac promoterAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Trpa1b-FLAG
Plasmid#106403PurposeMammalian expression vector containing zebrafish Trpa1b with C-terminal FLAG tag.DepositorInsertTrpa1b-FLAG (trpa1b Zebrafish)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
SRS-MTBD(26/19) AB(monomer), wt
Plasmid#22381DepositorInsertSeryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
UseTagsmycExpressionBacterialMutationPromoterAvailable sinceNov. 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_AVR-Pia
Plasmid#123653PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertAVR-Pia
UseGolden gateTagsExpressionMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only