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pMSCVpuro-T7-hAIM2-EGFP

Plasmid

#51608

Purpose

Express T7 and GFP-tagged hAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Use

Retroviral

Tags

EGFP and T7

Expression

Mammalian

Mutation

Promoter

MESV

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

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UAS::PTPRFb-DN-GFP

Plasmid

#37111

Depositor

Alvaro Sagasti

Article

Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub

Insert

UAS::PTPRFb-DN-GFP (ptprfb Zebrafish)

Use

Tol2 gateway expression vector

Tags

EGFP

Expression

Mutation

Deleted aa1386-1909 corresponding to phosphatase …

Promoter

10xUAS

Available since

July 18, 2012

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF4G_O

Plasmid

#147091

Purpose

Mammalian Expression of HseIF4G

Depositor

Elisa Izaurralde

Insert

HseIF4G (EIF4G1 Human)

Use

Tags

Expression

Mammalian

Mutation

one non silent mutation M432V compared to the seu…

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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[Bla][IRES][GFP]

Plasmid

#97193

Purpose

Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.

Depositor

Edward Boyden

Article

Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

Inserts

beta-Lactamase
GFP

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 16, 2017

Availability

Academic Institutions and Nonprofits only

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lag-2 promoter:GFP (pJK590)

Plasmid

#26814

Depositor

Judith Kimble

Article

Gao et al Proc Natl Acad Sci U S A. 1995 Oct 10. 9

Insert

lag-2 promoter

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Available since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only

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miniCMV-EGFP-tDeg

Plasmid

#185400

Purpose

EGFP-tDeg fluorogenic protein

Depositor

Jian-Hui Jiang

Article

Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

Insert

EGFP-tDeg

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-PA-GFP-kanMX6

Plasmid

#18820

Depositor

Wei-Lih Lee

Article

Vorvis et al Yeast. 2008 Sep;25(9):651-9.

Insert

Photoactivatable Green Fluorescent Protein

Use

Yeast tagging

Tags

Expression

Mutation

Promoter

Available since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pAG415GPD-EGFP-ccdB

Plasmid

#14314

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pBS500 EF1alpha-GFPcre

Plasmid

#11920

Depositor

Brian Sauer

Article

Gagneten et al Nucleic Acids Res. 1997 Aug 15. 25(16):3

Insert

GFP-cre (cre bacteriophage P1)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
pAG426GPD-ccdB-EGFP

Plasmid

#14204

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM

Plasmid

#183077

Purpose

Plasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c tracrRNA and crRNA ending with a HDV ribozyme

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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HA-BAP1-S63C-GFP

Plasmid

#78911

Purpose

Mammalian expression of BAP1 with S63E and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

Insert

BAP1 (BAP1 Human)

Use

Tags

EGFP and HA

Expression

Mammalian

Mutation

S63C

Promoter

CMV

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pT2-cryR;lmo2P1Egfp

Plasmid

#90162

Purpose

contains lmo2 specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

lmo2P1Egfp (lmo2 Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-UTRN-mini

Plasmid

#158749

Purpose

Expresses a shorter version of Utrophin (amino acid 35-136) with amino terminus eGFP in mammalian cells

Depositor

Minhajuddin Sirajuddin

Article

Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152

Insert

UTRN-mini

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-d2eGFP-cxcr4

Plasmid

#21967

Depositor

Phil Sharp

Article

Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only
pBI-EGFP-PUMA

Plasmid

#16590

Depositor

Bert Vogelstein

Article

Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

Insert

PUMA (BBC3 Human)

Use

Tags

2x HA

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pFX-EGFP-Rab7

Plasmid

#174457

Purpose

Express EGFP with late endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with late endosome-targeting sequence

Use

Tags

small GTPase (Rab7)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pORANGE Gria3-GFP KI

Plasmid

#131491

Purpose

Endogenous tagging of GluA3: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Gria3, Gria3 Mouse, Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_HaloTag9_T2A_EGFP

Plasmid

#169326

Purpose

Expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag9

Use

Tags

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET23-CBD-(-PP)-EGFP

Plasmid

#12597

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Insert

Cdc42 Biosensor (WAS Human)

Use

Tags

6xHis and EGFP

Expression

Bacterial

Mutation

F271C

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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