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pcDNA5-FRT-TO-NOD1-EGFP

Plasmid

#131206

Purpose

mammalian expression, tet R

Depositor

Thomas Kufer

Article

Pei et al EMBO J. 2021 Jul 1;40(13):e106272. doi:

Insert

NOD1 (NOD1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCW empty vector

Plasmid

#184708

Purpose

Lentiviral empty vector for doxycycline-inducible expression

Depositor

Gloria Manzotti, Alessia Ciarrocchi

Article

Gugnoni et al J Exp Clin Cancer Res. 2022 Mar 25;41(1)

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI D450A

Plasmid

#207953

Purpose

nuclease inactive control for TRF1FokI WT

Depositor

Roger Greenberg

Article

Dilley et al Nature. 2016 Nov 3;539(7627):54-58. doi:

Insert

TRF1 (TERF1 Human)

Use

Lentiviral

Tags

Flag, DD, ER, mCherry and FokI (D450A)

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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p415 TEF roGFP2‐Tsa2ΔCR

Plasmid

#83238

Purpose

The genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

Depositor

Tobias Dick

Article

Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

Insert

roGFP2-Tsa2dCr

Use

Tags

roGFP2 fusion with Tsa2dCr

Expression

Yeast

Mutation

Promoter

TEF

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO eNpHR 3.0-EYFP

Plasmid

#26966

Purpose

AAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibition

Depositor

Karl Deisseroth

Article

Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

eNpHR 3.0

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

ER export at 3' end of gene. Trafficking sig…

Promoter

EF1alpha

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pPAGFP-N1

Plasmid

#11909

Depositor

Jennifer Lippincott-Schwartz

Article

Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

Has Service

Cloning Grade DNA

Insert

pPAGFP-N1

Use

Tags

Expression

Mammalian

Mutation

These plasmids are essentially the same as the Cl…

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-R-Rac1

Plasmid

#180576

Purpose

ddRFP sensor of Rac1

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddRFP sensor of Rac1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCas9-HE

Plasmid

#109400

Purpose

Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein

Depositor

Jean-Paul Concordet

Article

Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

Insert

HDR-Enhancer domain of human CtIP protein (RBBP8 Human)

Use

Tags

Expression

Mutation

Promoter

Available since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pComb3XTT

Plasmid

#63891

Purpose

Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)

Depositor

Carlos Barbas

Article

Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

Insert

TT Fab

Use

Phage display

Tags

6xHis, HA tag, and geneIII

Expression

Bacterial

Mutation

Promoter

lacZ

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pUBC-Pfv-Sapphire_PURO

Plasmid

#203651

Purpose

Mammalian lentiviral vector for the expression of the Sapphire-tagged 40nm GEM under the UBC promoter, with a puromycin selection marker.

Depositor

Liam Holt

Article

Shu T et et al bioRxiv 2022

Insert

PfV

Use

Lentiviral

Tags

Sapphire

Expression

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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CoV2-N-P199L

Plasmid

#177949

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P199L). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (P199L) (N )

Use

Tags

Expression

Mammalian

Mutation

P199L

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-JAK1

Plasmid

#23932

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

JAK1 (JAK1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

R647H (numbering relative to NP_002218.1)

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pPAGFP-C1

Plasmid

#11910

Depositor

Jennifer Lippincott-Schwartz

Article

Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

Has Service

Cloning Grade DNA

Insert

PAGFP

Use

Tags

Expression

Mammalian

Mutation

These plasmids are essentially the same as the Cl…

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pSpliceExpress

Plasmid

#32485

Depositor

Stefan Stamm

Article

Kishore et al Gene. 2008 Dec 31;427(1-2):104-10. Epub

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-hChR2(H134R)-EYFP

Plasmid

#26969

Purpose

AAV expression of humanized ChR2(H134R) fused to EYFP driven by CaMKIIa promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Lee et al Nature. 2010 Jun 10. 465(7299):788-92.

Has Service

AAV1, AAV2, AAV5, and AAV9

Insert

hChR2(H134R)

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Histidine 134 is mutated to Arginine

Promoter

CaMKIIa

Available since

Feb. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-SV40NLSf-mScarletP2A-hLuc

Plasmid

#218793

Purpose

AAV reporter genome plasmid

Depositor

Ben Deverman

Article

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

NLSf-mScarletP2A-hLuc

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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Synapsin-cyto-mRuby3-iATPSnFR1.0

Plasmid

#102557

Purpose

expresses cytoplasmic ATP sensor in neurons with red reference protein

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

mRuby3-iATPSnFR1.0

Use

AAV

Tags

Ruby3

Expression

Mutation

Promoter

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_050

Plasmid

#96925

Purpose

lentiviral expression of gRNA scaffold

Depositor

David Root, John Doench

Article

Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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AiP1969 - pAAV-AiE2563m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214597

Purpose

AiE2563m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-s ONE-GO

Plasmid

#189731

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-s ONE-GO
GNAS (GNAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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