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Plasmid#171425PurposeFor the purifcation of phosphorylation mimic proteinDepositorInsertOPTN (OPTN Human)
UseTagsExpressionBacterialMutationphosphorylation mutant (S177D, S473D)PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST-TEVcs- FIP200 delta641-779-MBP
Plasmid#171411PurposeFor the purifcation of human ULK1 truncated complexDepositorInsertFIP200 (RB1CC1 Synthetic)
UseTagsExpressionMammalianMutationtruncation (delta641-779-MBP)PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGST-POLA1-N0
Plasmid#160983PurposeExpress a fragment of mouse Pola1 proteinDepositorInsertPOLA1 (Pola1 Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGST-POLA1-N1-2A
Plasmid#160986PurposeExpress a fragment of mouse Pola1 proteinDepositorInsertPOLA1 (Pola1 Mouse)
UseTagsGSTExpressionBacterialMutationW84A/D88APromoterAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGST-POLA1-N2-2A
Plasmid#160987PurposeExpress a fragment of mouse Pola1 proteinDepositorInsertPOLA1 (Pola1 Mouse)
UseTagsGSTExpressionBacterialMutationW84A/D88APromoterAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGST-POLA1-N2
Plasmid#160985PurposeExpress a fragment of mouse Pola1 proteinDepositorInsertPOLA1 (Pola1 Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGST-POLA1-N1
Plasmid#160984PurposeExpress a fragment of mouse Pola1 proteinDepositorInsertPOLA1 (Pola1 Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz106_CsoS2_MR
Plasmid#140843PurposeHis-tagged CsoS2 Middle Region (MR), pET-14b backboneDepositorInsertCsoS2 Middle Region (MR)
UseTagsHexahistidine tagExpressionBacterialMutationonly amino acids 257-603PromoterT7Available sinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz75_CB_operon_CsoS2_N3-N4
Plasmid#140857PurposeSame as pLz37 but with CsoS2 truncated such that NTD repeats N1 and N2 are removedDepositorInsertCbbL, CbbS, CsoS2 (delN1-N2), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
UseTagsExpressionBacterialMutationdeleted amino acids 1-109 of CsoS2PromoterAvailable sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz77_CB_operon_CsoS2_N3
Plasmid#140859PurposeSame as pLz37 but with CsoS2 discontinuously truncated such that repeats N1, N2, and N4 are removedDepositorInsertCbbL, CbbS, CsoS2 (delN1-N2, N4), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
UseTagsExpressionBacterialMutationdeleted amino acids 1-108 and 220-235 of CsoS2PromoterAvailable sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz78_CB_operon_CsoS2_delNTD
Plasmid#140860PurposeSame as pLz37 but with CsoS2 truncated to remove the entire NTD (i.e. N1, N2, N3, and N4 are removed)DepositorInsertCbbL, CbbS, CsoS2 (delNTD), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
UseTagsExpressionBacterialMutationdeleted amino acids 1-235 of CsoS2PromoterAvailable sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz76_CB_operon_CsoS2_N4
Plasmid#140858PurposeSame as pLz37 but with CsoS2 truncated such that NTD repeats N1, N2, and N3 are removedDepositorInsertCbbL, CbbS, CsoS2 (delN1-N3), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
UseTagsExpressionBacterialMutationdeleted amino acids 1-189 of CsoS2PromoterAvailable sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz109_CsoS2_NTD
Plasmid#140842PurposeHis-tagged CsoS2 N-terminal domain (NTD), pET-14b backboneDepositorInsertCsoS2 N-terminal domain (NTD)
UseTagsHexahistidine tagExpressionBacterialMutationdeleted amino acids 262-869PromoterT7Available sinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz33_CsoS2_NTD_GFP
Plasmid#140861PurposeHis-tagged CsoS2 N-terminal domain (NTD) fused to Superfolder GFPDepositorInsertCsoS2 NTD fused to GFP
UseTagsHexahistidine tag and Superfolder GFPExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz47_polyPro_CsoS2_Npeptide
Plasmid#140845PurposeConsensus N*-peptide after polyproline II helix, N-terminal His- tag used as BLI bait, pET-14b backboneDepositorInsertpolyproline helix with CsoS2 N-peptide
UseTagsHexahistidine tag and Strep-tag IIExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz110_CsoS2_CTD
Plasmid#140844PurposeHis-tagged CsoS2 C-terminal domain (CTD), pET-14b backboneDepositorInsertCsoS2 C-terminal domain (CTD)
UseTagsHexahistidine tagExpressionBacterialMutationdeleted amino acids 1-594PromoterT7Available sinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
FoxFTVC+bpFOG_MRAS-G22V
Plasmid#107521PurposeConstitutive activation of FGF/MAPK pathway by M-Ras-G22V mutantDepositorInsertM-Ras protein
UseTagsExpressionMutationchanged Glycine 22 to ValinePromoterTVC-specific FoxF enhancer (FoxFTVC+bpFOG)Available sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK234_047
Plasmid#124055PurposeEncodes the spCas9 mScarlet (site 2) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertmScarlet sgRNA 2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_048
Plasmid#124056PurposeEncodes the spCas9 mScarlet (site 3) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertmScarlet sgRNA 3
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_049
Plasmid#124057PurposeEncodes the spCas9 mScarlet (site 4) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertmScarlet sgRNA 4
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only