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pDDGFP-Leu2d-ScVrg4

Plasmid

#102334

Purpose

Expresses WT ScVrg4 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Parker et al Nature. 2017 Nov 23;551(7681):521-524. d

Insert

YGL225W (VRG4 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pTn7CdrA-gfp(ASV)C

Plasmid

#111617

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

Depositor

Tim Tolker-Nielsen

Article

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(ASV)-T0-T1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pRetroSuper-GFP Smad3

Plasmid

#15723

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

Smad3 shRNA (SMAD3 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pCAG-AkCas12b-2AeGFP

Plasmid

#121946

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AkCas12b

Use

Tags

3xHA tag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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NUMB2-eGFP-M107K

Plasmid

#37803

Depositor

Rolf Bjerkvig

Article

Euskirchen et al Exp Cell Res. 2011 Dec 10;317(20):2864-7

Insert

NUMB isoform 2 (NUMB Human)

Use

Lentiviral

Tags

eGFP

Expression

Mutation

Promoter

pCMV

Available since

March 11, 2013

Availability

Academic Institutions and Nonprofits only

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mPA-GFP-CD81-10

Plasmid

#57124

Purpose

Localization: Tetraspanin/Membrane, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Insert

CD81 (CD81 Human)

Use

Tags

mPA-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
qTAG-C-moxGFP-Zeo

Plasmid

#207732

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al bioRxiv

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pT3TS-miniTurboID-EGFP

Plasmid

#163847

Purpose

In vitro transcription of miniTurboID-EGFP. Parton lab clone JRT

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

miniTurbo

Use

In vitro transcription of rna

Tags

EGFP

Expression

Mutation

Promoter

Available since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCU-MET3-GFP

Plasmid

#45337

Depositor

Brendan Cormack

Article

Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

Insert

yeGFP

Use

C. glabrata expression

Tags

Expression

Mutation

Promoter

MET3

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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EGFP-uTEV1-MAVS

Plasmid

#170998

Purpose

HiLITR protease with MAVS c-terminal tmd targeting information (Mitochondria)

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-MAVS(tmd)

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

pTRE-tight (TetOn)

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSH231-Bx-GFP-C31

Plasmid

#115149

Purpose

Empty safe harbor site 231 knock-in vector with flanking homology arms and Bxb1 and C31 attP integrase landing pads

Depositor

Michael Phelps, Raymond Monnat

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

Safe Harbor Homology Sequences

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCW-eGFP-FHA

Plasmid

#114129

Purpose

Lentiviral vector for inducible expression of eGFP-RNF8 FHA domain fusion; 53BP1-independent recruitment to damaged chromatin

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

RNF8 FHA domain (amino acids 2-160) (RNF8 Human)

Use

Lentiviral; Doxycycline inducible

Tags

eGFP

Expression

Mammalian

Mutation

aa 2-160

Promoter

TRE promoter, Tet ON

Available since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-NDUFA7-mVenus

Plasmid

#185647

Purpose

NDUFA7 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFA7 (NDUFA7 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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mPA-GFP-Lifeact-7

Plasmid

#54671

Purpose

Localization: Actin, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

mPA-GFP-Lifeact

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pLEX303 GFP-PIK3C2A

Plasmid

#162000

Purpose

Lentiviral expression vector containing PIK3C2A tagged with GFP

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PIK3C2A (PIK3C2A Human)

Use

Lentiviral

Tags

eGFP

Expression

Mutation

Promoter

Available since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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mPA-GFP-MAPTau-N-10

Plasmid

#57146

Purpose

Localization: Microtubules, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Insert

MAPTau (MAPT Human)

Use

Tags

mPA-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST47-ARL1-GFP

Plasmid

#67395

Purpose

Mammalian expression of hArl1 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL1 (ARL1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

bp 231 C to T; silent mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pMito-PAGFP-FRB.

Plasmid

#60005

Purpose

Expresses MitoTrap tagged with photo-activatable GFP in mammalian cells.

Depositor

Stephen Royle

Article

Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

Type

Empty backbone

Use

Knocksideways

Tags

FRB and PAGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCMVd1-EGFP-hRhoA-wt

Plasmid

#133926

Purpose

Low level expression of GFP fused human RhoA wild type

Depositor

Sebastián Dupraz, Frank Bradke

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

GFP-hRhoA WT (RHOA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pUA66 theta GFP

Plasmid

#105607

Purpose

Expresses GFP with no promoter

Depositor

Pamela Silver

Article

Stirling et al Mol Cell. 2017 Nov 16;68(4):686-697.e3.

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

No Promoter

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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