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pcDNA3_HTR2A-NTEV-TCS-GV-2xHA

Plasmid

#194366

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

HTR2A-NTEV-TCS-GV-2xHA (HTR2A Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_DRD2-NTEV-TCS-GV-2xHA

Plasmid

#194362

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

DRD2-NTEV-TCS-GV-2xHA (DRD2 Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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His-TEV-SNAP-PRC1

Plasmid

#167541

Purpose

For expression of His6-TEV-SNAP-PRC1 in bacteria.

Depositor

Tarun Kapoor

Article

Subramanian et al Cell. 2013 Jul 18;154(2):377-90. doi: 10

Insert

PRC1 (PRC1 Human)

Use

Tags

His6 tag and SNAP tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pOmniBac zz TEV YBBR TRF1

Plasmid

#185445

Purpose

Expresses human TRF1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cells

Depositor

Ahmet Yildiz

Article

Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

Insert

TRF1 (TERF1 Human)

Use

Tags

YBBR and ZZ

Expression

Insect

Mutation

Promoter

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2408-Tier1-PhCMV-TEVsite

Plasmid

#169503

Purpose

Tier-1 vector encoding PhCMV-controlled tabacco etch virus protease recognition site (PhCMV-TEVsite-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven tobacco etch virus protease cleavage site

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2406-Tier1-PhCMV-NTEV

Plasmid

#169501

Purpose

Tier-1 vector encoding PhCMV-driven N-terminal fragment of a split tabacco etch virus protease splitTEV (PhCMV-N-TEV-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven N-terminal fragment of split tobacco etch virus protease

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2405-Tier1-PhCMV-TEV

Plasmid

#169500

Purpose

Tier-1 vector encoding PhCMV-driven tabacco etch virus protease TEV (PhCMV-TEV-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven tobacco etch virus protease

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX2T-TEV CHMP1B 4-178

Plasmid

#108284

Purpose

Expresses residues 4-178 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-279

Depositor

Wesley Sundquist

Article

Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

Insert

CHMP1B (CHMP1B Human)

Use

Tags

GST

Expression

Bacterial

Mutation

deleted amino acids 1-3 and 179-199

Promoter

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX2T-TEV CHMP1B 1-143

Plasmid

#108286

Purpose

Expresses residues 1-143 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-278

Depositor

Wesley Sundquist

Article

Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

Insert

CHMP1B (CHMP1B Human)

Use

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-143

Promoter

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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His-TEV-GB1 Ile6Met

Plasmid

#128687

Purpose

Expresses GB1 Ile6Met mutant

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
His-TEV-GB1 Val29Met

Plasmid

#128688

Purpose

Expresses GB1 Val29Met mutant

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
His-TEV-GB1 Ala34Met

Plasmid

#128689

Purpose

Expresses GB1 Ala34Met mutant

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
His-TEV-GB1 Val39Met

Plasmid

#128690

Purpose

Expresses GB1 Val39Met mutant

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
His-TEV-GB1 Val54Met

Plasmid

#128691

Purpose

Expresses GB1 Val54Met mutant

Depositor

Sharon Rozovsky

Article

Chen et al J Phys Chem B. 2020 Jan 30;124(4):601-61

Insert

spg

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
CMVTO-RBDK65E-R89L-nTEVp

Plasmid

#179670

Purpose

Mutant Ras Binding Domain split-nTEVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

CRaf RBD protein (RAF1 Human)

Use

Tags

nTEVP

Expression

Mammalian

Mutation

K65E, R89L

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-RBDK65E-R89L-cTEVp

Plasmid

#179671

Purpose

Mutant Ras Binding Domain split-cTEVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

CRaf RBD protein (RAF1 Human)

Use

Tags

cTEVP

Expression

Mammalian

Mutation

K65E, R89L

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pACEBac1-ZZ-TEV-MZT2-3C-mEGFP

Plasmid

#178074

Purpose

Insect cell expression of ZZ-TEV-MZT2-3C-mEGFP

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

MZT2A (MZT2A Human)

Use

Tags

PreScission-mEGFP and ZZ-TEV

Expression

Insect

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI2dR108E-cs-TEV-STREP

Plasmid

#171835

Purpose

Mammalian expression of human WIPI2d R108E with C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Use

Tags

Strep

Expression

Mammalian

Mutation

R108E

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI2dR125E-cs-TEV-STREP

Plasmid

#171836

Purpose

Mammalian expression of human WIPI2d R125E with C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Use

Tags

Strep

Expression

Mammalian

Mutation

R125E

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-WIPI2dK128E-cs-TEV-STREP

Plasmid

#171837

Purpose

Mammalian expression of human WIPI2d K128E with C-terminal Strep

Depositor

James Hurley

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2d (WIPI2 Human)

Use

Tags

Strep

Expression

Mammalian

Mutation

K128E

Promoter

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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