Addgene

Showing: 501 - 520 of 11257 results

Results per page:

Sort results by:

pcDNA3.1-GAVPO

Plasmid

#159923

Purpose

GAVPO (VIVID transactivator) expressed under the CMV promoter in the pcDNA3.1 backbone. AKA 133

Depositor

Fei Chen

Article

Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

Insert

GAVPO

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_T6B -mCherry

Plasmid

#205600

Purpose

expresses human T6B peptide fused with mChery in mammalian cells

Depositor

Marc Friedlander

Article

Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

Insert

T6B peptide (fragment of human TRNC6B 599-683aa) (TNRC6B Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1-H-Luc_cyto

Plasmid

#113929

Purpose

Plasmid encoding the cytosolic localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

H-Luc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.3_CoV2_B.1.1.7

Plasmid

#170451

Purpose

Express SARS-CoV-2 B.1.1.7 (UK strain / alpha strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 (UK strain) pseudotyped virus production.

Depositor

David Nemazee

Article

Yuan et al Science. 2021 Aug 13;373(6556):818-823.

Insert

SARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, UK strain (B.1.1.7))

Use

Tags

NA

Expression

Mammalian

Mutation

HY69-70del, Y144del, N501Y, A570D, D614G, P681H, …

Promoter

CMV

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-mNeptune2.5

Plasmid

#51310

Purpose

Expresses far-red fluorescent protein mNeptune2.5 in mammalian cells

Depositor

Michael Lin

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

mNeptune2.5

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 8, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-hNox5

Plasmid

#69354

Purpose

expresses human Nox5 in mammalian cells

Depositor

Karl-Heinz Krause

Article

Banfi et al J Biol Chem. 2001 Oct 5;276(40):37594-60

Insert

hNox5 (NOX5 Human)

Use

Tags

no

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcdna nesprin HL

Plasmid

#68128

Purpose

nesprin headless control

Depositor

Daniel Conway

Article

Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

Insert

nesprin-2 (Syne2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

deleted actin-binding domain

Promoter

CMV

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
ReNL-H2B_pcDNA3

Plasmid

#89534

Purpose

Red color variant of bright luminescent protein, fused to histone 2B

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Use

Tags

histone 2B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3_NSnoFbox-vhhGFP4

Plasmid

#35580

Depositor

Markus Affolter

Article

Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

Insert

NSnoFbox-vhhGFP4 (slmb Fly)

Use

Tags

Expression

Mammalian

Mutation

F box domain of slmb deleted.

Promoter

Available since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-mRuby4(1-10)

Plasmid

#165143

Purpose

Expresses mRuby4(1-10) in mammalian cells

Depositor

Daichi Kamiyama

Article

Tamura et al Commun Biol. 2021 Feb 26;4(1):257. doi:

Insert

mRuby4(1-10)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
HA-UBE2E1 WT in pcDNA3.1

Plasmid

#118213

Purpose

Expresses HA-UBE2E1 in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

UBE2E1 (UBE2E1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

none

Promoter

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
C1(1-29)-miniTurbo-V5_pCDNA3

Plasmid

#107174

Purpose

expresses V5-tagged miniTurbo on the mammalian ER membrane

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Use

Tags

Expression

Mammalian

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1-H-Luc_mito

Plasmid

#113930

Purpose

Plasmid encoding the mictochonfrial localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

H-Luc_mito

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mito-gTEMP_pcDNA3

Plasmid

#109117

Purpose

Mitochondria targetting gTEMP

Depositor

Takeharu Nagai

Article

Nakano et al PLoS One. 2017 Feb 17;12(2):e0172344. do

Insert

mito-gTEMP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3 WT B-Myb

Plasmid

#25965

Depositor

Rob Lewis

Article

Johnson et al J Biol Chem. 1999 Dec 17. 274(51):36741-

Insert

B-Myb (MYBL2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-GBF1

Plasmid

#206415

Purpose

Mammalian expression of hGBF1

Depositor

Kazuhisa Nakayama

Article

Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

Insert

GBF1 (GBF1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-IL-2 signal_CyPet

Plasmid

#128029

Purpose

Mammalian expression vector with N terminal IL-2 signal peptide and CyPet

Depositor

Oskar Laur

Type

Empty backbone

Use

Tags

CyPet and IL-2 signal peptide

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNAintron-EBOV-GP

Plasmid

#215692

Purpose

Produces the Ebola virus glycoprotein

Depositor

Melinda Brindley

Article

Acciani et al J Virol. 2021 Sep 27;95(20):e0116521. do

Insert

EBOV-GP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA3.1-HA-Bax

Plasmid

#191944

Purpose

Expression of HA-Bax.

Depositor

Luca Scorrano

Insert

HA-Bax (Bax Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 Leo1

Plasmid

#11061

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

Leo1 (LEO1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge