Showing: 501 - 520 of 1781 results
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Plasmid#177733PurposeEleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged in yeast plasmid with URA markerDepositorInsertMTS-GFP11
UseTagsExpressionBacterial and YeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
U6-NT sgRNA; EF1a-dCas9-KRAB-GFP
Plasmid#194284PurposeEF1a-dCas9-KRAB-GFP with nontarget sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
p406 – TDH3-ER-FlucDM-GFP- Ura
Plasmid#177732PurposeDM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention and fused to eGFP at the C-terminus in yeast plasmid with URA markerDepositorInsertFlucDM
UseTagsHA, HDEL, KAR2 Signal Sequence, and eGFPExpressionBacterial and YeastMutationR188Q, R261Q (Destabilized Mutant)PromoterAvailabilityAcademic Institutions and Nonprofits only -
p406 – TDH3-Elo3-GFP11-Ura
Plasmid#177734PurposeER protein Elo3 with a C-terminal, an HA tag and the eleventh β-strand of GFP in yeast plasmid with URA markerDepositorInsertElo3 (ELO3 Budding Yeast)
UseTagsGFP11 and HDELExpressionBacterial and YeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEF NS34a-msGFP-A'Q-mito
Plasmid#158763PurposeExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseTagsExpressionMammalianMutationFlexible linkers between the protease and msGFP a…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn NS34a-msGFP-A'Q-mito
Plasmid#158766PurposeLentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseLentiviralTagsExpressionMutationFlexible linkers between the protease and msGFP a…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pLenti-C-STAT1-mGFP-P2A-Puro
Plasmid#199345Purposeexpress murine STAT1 protein fused with mGFPDepositorInsertsignal transducer and activator of transcription 1 (Stat1 Mouse)
UseLentiviralTagsmGFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pET-45b-NFYC-IDR-mEGFP
Plasmid#185015PurposeBacterial Expression of 6xHis NFYC IDR Sirius-GFP Fusion ProteinDepositorInsertNFYC IDR (Nfyc Mouse)
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFastBac-M13-6His-KIF1C_P176L-GFP
Plasmid#185978PurposeBaculovirus expression of human KIF1C with P176L mutation and 6xHis and GFP tags.DepositorInsertKIF1C (KIF1C Human)
UseTags6xHis and eGFPExpressionInsectMutationP176L - mutation causing HSP (SPG58)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pFastBac-M13-6His-KIF1C_R169W-GFP
Plasmid#185979PurposeBaculovirus expression of human KIF1C with R169W mutation and 6xHis and GFP tags.DepositorInsertKIF1C (KIF1C Human)
UseTags6xHis and eGFPExpressionInsectMutationR169W - mutation causing HSP (SPG58)PromoterAvailabilityAcademic Institutions and Nonprofits only -
EF1a-dCas9-KRAB-GFP backbone
Plasmid#194281PurposeVector for CRISPRi-GFP expression ready for gateway cloning of sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pPB-Neo-COVGT1-d2EGFP-mCherry
Plasmid#201451PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT1_d2EGFP-mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pPB-Neo-COVGT2-d2EGFP-mCherry
Plasmid#201452PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT2_d2EGFP-mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pPB-Neo-COVGT4-d2EGFP-mCherry
Plasmid#201453PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT4_d2EGFP-mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pPB-Neo-COVGT5-d2EGFP-mCherry
Plasmid#201454PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT5_d2EGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
p406 – TDH3-ER-FlucDM-GFP11-Ura
Plasmid#177731PurposeDM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus in yeast plasmid withDepositorInsertFlucDM
UseTagsGFP11, HA, HDEL, and KAR2 Signal SequenceExpressionBacterial and YeastMutationR188Q, R261Q (Destabilized Mutant)PromoterAvailabilityAcademic Institutions and Nonprofits only -
GFP-APPL1-R146A/K152A/R154A
Plasmid#59767PurposeExpresses APPL1 Endosomal Localization MutantDepositorInsertAPPL1 (APPL1 Human)
UseTagsEGFPExpressionMammalianMutationR146A/K152A/R154APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-Ensconsin-3XGFP-stop-L2
Plasmid#186361PurposeEntry clone with ORF encoding the MT-binding domain of Ensconsin fused to 3 copies of GFP in C-terminal, flanked by Gateway recombination sequences.DepositorInsertHuman Ensconsin (MAP7 Human, Synthetic)
UseExpression of a fluorescent microtubule markerTags3xEGFPExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGal-Trans-Myc-eGFP-KDEL
Plasmid#177729PurposeER protein collagen beta (1-O) galactosyltransferase fused to eGFP at the C-terminus, myc taggedDepositorInsertcollagen beta(1-O)galactosyltransferase 1 (COLGALT1 Human)
UseTagsKDEL, Myc, and eGFPExpressionBacterial and MammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
RUSH reporter-HMGB1-SBP-GFP
Plasmid#172357PurposeMonitoring of HMGB1 subcellular localization by fluorescence videomicroscopyDepositorInserthigh mobility group box 1 (HMGB1 Human)
UseLentiviralTagsSBP-GFPExpressionMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 501 - 520 of 1781 results