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Plasmid#166053Purposea caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but has CARD domain and function as an initiator caspaseDepositorInsertOrbicella faveolata caspase-3a
UseTagsHis tagExpressionBacterialMutationPromoterT7 lac promoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3.2mar-Luc
Plasmid#63696Purpose3.2 kb marine armor plate enhancer driving Luciferase expressionDepositorInsert3.2 kb marine stickleback armor plate enhancer
UseTagsLuciferaseExpressionMammalianMutationPromoterTATA boxAvailable sinceApril 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJK412
Plasmid#155371PurposedCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]DepositorInsertsdCas9 (bacteria)
PA4-mVenus
dCas9 sgRNA oscillator v1
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
M50 Super 8x TOPFlash
Plasmid#12456PurposeBeta-catenin reporter. TCF/LEF sites upstream of a luciferase reporter.DepositorInsertTCF/LEF binding sites (Ctnnb1 )
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3_SEPT6
Plasmid#71583Purposemammalian expression of human SEPT6 with a GFP fusionDepositorInsertSeptin 6 (SEPTIN6 Human)
UseTagseGFPExpressionMammalianMutationV206DPromoterCMVAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJK511
Plasmid#155384PurposedCas12a oscillator, reverse direction crRNAs. dCas12a (D917A) + PA4-mVenusDepositorInsertsdCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-FLNa WT
Plasmid#8982DepositorInsertFilamin A (FLNA Human)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2005AvailabilityAcademic Institutions and Nonprofits only -
DR5rev::erRFP
Plasmid#61011PurposeAuxin signalling reporter (DR5rev) driving RFP (ER-targeted)DepositorInsertDR5rev::erRFP:35S_term
UseTagsExpressionPlantMutationPromoterDR5rev::erRFP:35StermAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pet28a-His6-Keap1
Plasmid#62454PurposeHis6 tagged Keap1 for E.coli expressionDepositorInsertKeap1 (KEAP1 Human)
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAR4-NLS
Plasmid#138217PurposeNuclear-targeted FRET-based PKA activity reporter.DepositorInsertAKAR4-NLS
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK503
Plasmid#155380PurposedCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenusDepositorInsertsdCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailable sinceMay 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-3xflagGREB1a
Plasmid#112221Purposemammalian expression of flag tagged GREB1aDepositorInsertGREB1a (GREB1 Human)
UseTags3x flagExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCmEos3.2:cat
Plasmid#78100PurposePlasmid template that encodes the photoconvertible fluorescent protein mEos3.2 next to chloramphenicol resistance gene (cat). This template was used to copy out mEos3.2-cat for genetic tagging.DepositorInsertmEos3.2-cat
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pet28a-His6-Halo-Tev-Keap1
Plasmid#62456PurposeHis6-halo tagged human Keap1 protein for E.coli expressionDepositorInsertKeap1 (KEAP1 Human)
UseTagsHis-Halo-TevExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-p97-EQ-mycStrep
Plasmid#31838DepositorInsertp97
UseTagsStrep and mycExpressionBacterial and MammalianMutationE578QPromoterCMVAvailable sinceOct. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
PH-miRFP-FRB
Plasmid#193568PurposeExpresses PH domain from PLC delta1 tagged with miRFP703 and FRB mutant (T2098L) in mammalian cells.DepositorInsertPH domain from PLC delta1 (Plcd1 Rat)
UseTagsFRB (T2098L) and miRFP703ExpressionMammalianMutationPH domainPromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-p97wt-mycStrep
Plasmid#31837DepositorInsertp97
UseTagsStrep and mycExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pIRESpuro2-UBXD1-3x HA
Plasmid#31836DepositorInsertUBXD1 (UBXN6 Human)
UseTags3x HAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
p156 pWZL-EGFP-SLPI
Plasmid#11069DepositorInsertSLPI (Slpi Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMIR-Dsred-IRES-His Keap
Plasmid#62457Purposeexpresses his tagged human Keap1 proteinDepositorInsertKeap1 (KEAP1 Human)
UseTagsHisTagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only