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Plasmid#124618PurposeExpresses SpyDock to bind SpyTag non-covalently for affinity purificationDepositorInsertSpyDock
UseTagsHis6ExpressionBacterialMutationE77A mutation prevents isopeptide bond formation,…PromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003 S49C
Plasmid#133448PurposeExpresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical couplingDepositorInsertSpyCatcher003 S49C
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationContains S49C mutationPromoterT7Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
AviTag-SpyTag003-MBP
Plasmid#184227PurposeExpresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.DepositorInsertAviTag-SpyTag003-MBP
UseTagsAviTag, His6, SpyTag003, and Thrombin cleavage si…ExpressionBacterialMutationPromoterT7Available sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-Talin rod-mCherry
Plasmid#133567PurposeExpresses SpyTag003-Talin rod-mCherry in mammalian cellsDepositorInsertSpyTag003-Talin rod-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Talin head-SpyCatcher003
Plasmid#133566PurposeExpresses EGFP-Talin head-SpyCatcher003 in mammalian cellsDepositorInsertEGFP-Talin head-SpyCatcher003
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyTag002 DA-MBP
Plasmid#102832PurposeBacterial expression of unreactive version of SpyTag002 fused to maltose binding proteinDepositorInsertSpyTag002 DA-MBP
UseTagsHis6 and Thrombin cleavage siteExpressionBacterialMutationReactive aspartic acid has been mutated to an ala…PromoterT7Available sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
TfR-sfGFP-myc tag-SpyCatcher003
Plasmid#133451PurposeExpresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detectionDepositorInsertTfR-sfGFP-myc tag-SpyCatcher003
UseTagsGSSGS and myc tagExpressionMammalianMutationContains C20 and A23 mutations that improve plasm…PromoterCMVAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpySwitch
Plasmid#184225PurposeExpresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.DepositorInsertSpySwitch
UseTagsHis6ExpressionBacterialMutationE77A mutation prevents isopeptide bond formation,…PromoterT7Available sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-FLAG-MKK6
Plasmid#21582DepositorInsertMKK6 (MAP2K6 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-FLAG-MKK6-K82M
Plasmid#21583DepositorInsertMKK6 (MAP2K6 Human)
UseTagsFLAGExpressionMammalianMutationlysine 82 to methioninePromoterAvailable sinceNov. 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCR-HA-IKKbeta
Plasmid#15470DepositorInsertIkB kinase beta (Ikbkb Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCR-Flag-IKKbeta
Plasmid#15465DepositorInsertIkB kinase beta (Ikbkb Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCR-HA-IKKalpha
Plasmid#15469DepositorInsertIkB kinase alpha (Chuk Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCR-Flag-IKKalpha
Plasmid#15467DepositorInsertIkB kinase alpha (Chuk Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCR-Flag-IKKalpha-KM
Plasmid#15468DepositorInsertIkB kinase alpha-KM (Chuk Mouse)
UseTagsFlagExpressionMammalianMutationchanged Lysine at 44 to AlaninePromoterAvailable sinceOct. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCR-Flag-IKKbeta-KM
Plasmid#15466DepositorInsertIkB kinase beta-KM (Ikbkb Mouse)
UseTagsFlagExpressionMammalianMutationchanged Lysine at 44 to AlaninePromoterAvailable sinceOct. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCR-Myc-IKKalpha
Plasmid#19739DepositorInsertIkB kinase alpha (Chuk Mouse)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCR-HA-IKKalphad17
Plasmid#19738DepositorInsertIkB kinase alpha (Chuk Mouse)
UseTagsHAExpressionMammalianMutationSplice variant lacking 17 amino acids at the C-te…PromoterAvailable sinceNov. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 MEKK4alpha KD
Plasmid#12188DepositorInsertMEKK4 (Map3k4 Mouse)
UseTagsHAExpressionMammalianMutationKinase dead: K1361MPromoterAvailable sinceSept. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pNK1198
Plasmid#219740PurposeMoClo-compatible Level 0 - 3U + terminator vector carrying terminator HSP18.2DepositorInsertThe heat shock protein 18.2 (HSP) terminator
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only