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YIp ADE2-LEU2

Plasmid

#122905

Purpose

ADE2-LEU2 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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YIp ADE2-URA3

Plasmid

#122907

Purpose

ADE2-URA3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 24, 2021

Availability

Academic Institutions and Nonprofits only

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YIp LYS2-HIS3

Plasmid

#122908

Purpose

LYS2-HIS3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-PIP5K1A_IDG-K

Plasmid

#214462

Purpose

Gateway destination clone of PIP5K1A (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-PIP5K1A (PIP5K1A Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pICE URA3

Plasmid

#122882

Purpose

URA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-HIS3

Plasmid

#122912

Purpose

TRP1-HIS3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCoofy51

Plasmid

#122005

Purpose

Baculovirus vector for parallel SLIC cloning containing N-terminal TwinStrep with PreScission Cleavage site

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Use

Tags

TwinStrep

Expression

Insect

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-HphMx

Plasmid

#122922

Purpose

TRP1-HphMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 4, 2019

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-KanMx

Plasmid

#122923

Purpose

TRP1-KanMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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YIp ADE2-NatMx

Plasmid

#122918

Purpose

ADE2-NatMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp TRP1-NatMx

Plasmid

#122924

Purpose

TRP1-NatMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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YIp ADE2-MET17

Plasmid

#122906

Purpose

ADE2-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp TRP1-URA3

Plasmid

#122915

Purpose

TRP1-URA3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp ADE2-KanMx

Plasmid

#122917

Purpose

ADE2-KanMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 27, 2021

Availability

Academic Institutions and Nonprofits only

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YIp LYS2-HphMx

Plasmid

#122919

Purpose

LYS2-HphMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp LYS2-NatMx

Plasmid

#122921

Purpose

LYS2-NatMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCoofy52

Plasmid

#122006

Purpose

E.coli vector for parallel SLIC cloning containing N-terminal His6-3xAvi with PreScission Cleavage site

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Use

Tags

His6-3xAvi

Expression

Bacterial

Mutation

Promoter

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCoofy9

Plasmid

#122004

Purpose

E.coli vector for parallel SLIC cloning containing N-terminal StrepII with PreScission Cleavage site

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Use

Tags

StrepII

Expression

Bacterial

Mutation

Promoter

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_phN

Plasmid

#223858

Purpose

4G-cloning donor for insect promoter and terminator, N-terminal tag added

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

polyhedrin promoter and SV40 polyA

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_3C-TS-His10

Plasmid

#223893

Purpose

4G-cloning donor for C-terminal 3C-TwinSTrep-His10 tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

3C-TwinStrep-His10

Use

Golden-gate donor

Tags

Expression

Mutation

Promoter

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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