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pSLQ8636 PB-PGK-RR12EE345L-C term (406) dLbCpf1-N intein-pA

Plasmid

#140223

Purpose

Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to intein for 3-way split activator

Depositor

Stanley Qi

Article

Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

Insert

zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to N-intein

Use

Tags

Expression

Mammalian

Mutation

D832A

Promoter

PGK

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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SacI ML GFP Strand 11 Long

Plasmid

#164120

Purpose

Detect the long Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

Depositor

Marisa Brini, Tito Calì

Article

Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

Insert

SacI ML GFP Strand 11 Long

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAT9752-BEAR-mScarlet

Plasmid

#162991

Purpose

BEAR target plasmid with split mScarlet and disrupted 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

mScarlet split with an intron between amino acids 110-111

Use

CRISPR

Tags

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV

Available since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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SacI ML GFP Strand 11 Short

Plasmid

#164121

Purpose

Detect the short Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

Depositor

Marisa Brini, Tito Calì

Article

Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

Insert

SacI ML GFP Strand 11 Short

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-SHTN1L

Plasmid

#209560

Purpose

Mammalian expression of Shtn1 long isoform

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 long isoform (4930506M07Rik Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA GFP10-RHOB

Plasmid

#182237

Purpose

Expresses RHOB GTPase with tripartite split GFP10 tag

Depositor

Stéphanie Cabantous

Article

Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

Insert

ras homolog family member B (RHOB Human)

Use

Tags

GFP10 (split-GFP tripartite)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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Hes5-Luc

Plasmid

#41724

Depositor

Ryoichiro Kageyama, Raphael Kopan

Article

Nishimura et al Genomics. 1998 Apr 1;49(1):69-75.

Insert

Hes5 Promoter (Hes5 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Contains the murine Hes5 promoter (-800 to +73)

Promoter

Hes5

Available since

Feb. 1, 2013

Availability

Academic Institutions and Nonprofits only
pUC19-TSV (linearized in LT)

Plasmid

#70033

Purpose

pUC19-TSV (linearized in LT) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the Large T-antigen coding region

Depositor

Mariet Feltkamp

Article

van der Meijden et al PLoS Pathog. 2010 Jul 29;6(7):e1001024.

Insert

TSPyV full genome, linearized in Large T-antigen

Use

Cloning vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-CAGGS-mNAGA

Plasmid

#196448

Purpose

Expresses modified α-N-acetylgalactosaminidase in mammalian cells

Depositor

Yuichiro Miyaoka

Article

Nakajima et al Cell Transplant. 2023 Jan-Dec;32:9636897

Insert

modified alpha-N-acetylgalactosaminidase (NAGA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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dCas9 fused to scFv (anti-spike)

Plasmid

#186421

Purpose

Expression of dCas9 with C-terminal nanobody fusion recognizing spike protein from SARS-CoV-2

Depositor

Stephen Elledge

Article

Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

Insert

dCas9-scFv fusion (anti-SARS-CoV-2 spike)

Use

CRISPR

Tags

6His

Expression

Mutation

Promoter

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-SHTN1S

Plasmid

#209561

Purpose

Mammalian expression of Shtn1 short isoform

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 short isoform (4930506M07Rik Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pHW564 (Pmyo-2::cGAL-N(C1A)::let-858 3'UTR)

Plasmid

#107739

Purpose

mutated cGAL-N (C1A) driver under the control of C. elegans myo-2 promoter

Depositor

Paul Sternberg

Article

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::cGAL(DBD)::gp41-1-N-intein(C1A)

Use

Tags

Expression

Worm

Mutation

change the first cysteine of gp41-1 N-intein to a…

Promoter

C. elegans myo-2 promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminator

Plasmid

#158585

Purpose

Expresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiae

Depositor

Cynthia Kenyon

Article

Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

Insert

membrane localization signal-tagBFP-wrmScarlet11

Use

Tags

tagBFP

Expression

Yeast

Mutation

GGGS linker

Promoter

TEF

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pET22b-T7-6h-GST-α-Synuclein

Plasmid

#225224

Purpose

Alpha synuclein gene fused with gst gene under t7 promoter for bacterial expression of alpha synuclein protein.

Depositor

Urartu Seker

Article

Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

Inserts

GST
α-Synuclein

Use

Tags

6xHis Tag and TEV Cleavage Site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-deltaExon17

Plasmid

#209563

Purpose

Mammalian expression of Shtn1 long isoform with E17 deletion

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 long isoform with E17 deletion (4930506M07Rik Mouse)

Use

Tags

Expression

Mammalian

Mutation

Exon17 deletion

Promoter

Available since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-SHTN1LRRR>GGG

Plasmid

#209562

Purpose

Mammalian expression of Shtn1 long isoform with RG mutation

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 long isoform with RG mutation (4930506M07Rik Mouse)

Use

Tags

Expression

Mammalian

Mutation

RRR to GGG

Promoter

Available since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-deltaExon16-17

Plasmid

#209564

Purpose

Mammalian expression of Shtn1 long isoform with E16-17 deletion

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 long isoform with E16-17 deletion (4930506M07Rik Mouse)

Use

Tags

Expression

Mammalian

Mutation

Exon16 and 17 deletion

Promoter

Available since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-deltaExon15-II

Plasmid

#209566

Purpose

Mammalian expression of Shtn1 long isoform with E15 deletion II

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 long isoform with E15 deletion II (4930506M07Rik Mouse)

Use

Tags

Expression

Mammalian

Mutation

Exon15 deletion II

Promoter

Available since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGIG-FLAG-SHTN1L

Plasmid

#209568

Purpose

Mammalian expression of Shtn1 long isoform

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 long isoform (4930506M07Rik Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGIG-FLAG-SHTN1S

Plasmid

#209569

Purpose

Mammalian expression of Shtn1 short isoform

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 short isoform (4930506M07Rik Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge