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pCS2-F1-GGA-F2-GTG-F3-TTG

Plasmid

#30701

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 GGA, F2 GTG, F3 TTG

Use

Mrna production

Tags

FLAG

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F1-GTG-F2-GGT-F3-GAA

Plasmid

#30707

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 GTG, F2 GGT, F3 GAA

Use

Mrna production

Tags

HA

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F1-GAG-F2-GAG-F3-AAG

Plasmid

#30695

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 GAG, F2 GAG, F3 AAG

Use

Mrna production

Tags

FLAG

Expression

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
XE226 Drosophila Chibby-pCS2P+

Plasmid

#16822

Depositor

Randall Moon

Article

Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

Insert

Drosophila Chibby (Cby Fly)

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
ZE44 myc-Dapper-2 pCS2P+

Plasmid

#17085

Depositor

Randall Moon

Article

Waxman et al Development. 2004 Dec. 131(23):5909-21.

Insert

Dapper-2 (dact2 Zebrafish)

Use

Zebrafish expression

Tags

myc

Expression

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
p031 pCS2 End-1 ORF

Plasmid

#11030

Depositor

Ramesh Shivdasani

Article

Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

Insert

End-1 (end-1 Nematode)

Use

Xenopus, mammalian, avian, and zebrafish

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pCS2 Notch1 Full Length-6MT

Plasmid

#41728

Depositor

Raphael Kopan

Article

Schroeter et al Nature. 1998 May 28;393(6683):382-6.

Insert

Notch-1 Full Length (Notch1 Mouse)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

L1997V; Contains murine Notch-1 protein up to ami…

Promoter

Available since

Dec. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pcs2-ATF4uORF1-2-GFP-IRES-mCherry

Plasmid

#226105

Purpose

ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)

Depositor

Michael Rape

Article

Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

Insert

atf4 (ATF4 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

contains uORF1-2 of ATF4, whose stability can ser…

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+_HA-mAID-nanobody (Kless)

Plasmid

#117718

Purpose

mRNA synthesis of lysine-less HA-mAID-nanobdy for injection

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

mAID-nanobody

Use

Tags

3xHA

Expression

Mammalian

Mutation

codon-optimized and lysine-less mAID for murine e…

Promoter

SP6

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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PCS2-FlipGFP(Casp3 cleavage seq)

Plasmid

#124432

Purpose

Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter in zebrafish (verified)

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipGFP(Casp3 cleavage seq)

Use

Zebrafish (good expression)

Tags

Expression

Mutation

Promoter

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcs2-HRI-GFP-IRES-mCherry

Plasmid

#226099

Purpose

HRI stability reporter construct for transient expression in mammalian cells

Depositor

Michael Rape

Article

Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

Insert

HRI (EIF2AK1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-MT mouse Axin (Axin MTFu1)

Plasmid

#21287

Depositor

Frank Costantini

Article

Zeng et al Cell. 1997 Jul 11. 90(1):181-92.

Insert

Axin (Axin1 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2009

Availability

Academic Institutions and Nonprofits only

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pCS2-3xFLAG-NLS-SpCas9-NLS

Plasmid

#51307

Purpose

Cas9 expression plasmid

Depositor

Yonglong Chen

Article

Guo et al Development. 2014 Jan 8.

Insert

3xFLAG-NLS-SpCas9-NLS

Use

CRISPR; Xenopus tropicalis expression

Tags

3x Flag and NLS

Expression

Mutation

Promoter

CMV

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2+_Flag-myc-NES-Tir1

Plasmid

#117717

Purpose

mRNA synthesis of Flag-myc-NES-TIR1 for injection

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

TRANSPORT INHIBITOR RESPONSE 1

Use

Tags

Flag-Myc-NES

Expression

Mammalian

Mutation

codon optimized TIR1 for murine expression

Promoter

SP6

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2 Halo-Tev- HSPB7 (zebrafish) WT

Plasmid

#108916

Purpose

Express WT Zebrafish Halo-Tev-HSPB7 in mammalian cells

Depositor

Yimon Aye

Article

Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

Insert

Halo-TEV-HSPB7 (hspb7 Zebrafish)

Use

Tags

Halo-TEV

Expression

Mammalian

Mutation

Promoter

SP6

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mCerulean 156-239-GGGS

Plasmid

#162616

Purpose

Allows for transcription of control mCerulean fluorophore half for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mCerulean

Use

Tags

Expression

Mammalian

Mutation

156-239

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-I152L-GGGS

Plasmid

#162613

Purpose

Allows for transcription of improved control mVenus I152L fluorophore half for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mVenus

Use

Tags

Expression

Mammalian

Mutation

1-155-I152L

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-FLAG-hEcto/Tif1g-CAmut

Plasmid

#20903

Depositor

Stefano Piccolo

Article

Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Insert

Ectodermin (TRIM33 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Point mutagenesis of C125 and C128 to Alanine (RI…

Promoter

Available since

May 15, 2009

Availability

Academic Institutions and Nonprofits only
pCS2 Flag Smad3 EPSM V179T

Plasmid

#26998

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Expression

Mutation

T179V S204A S208A S213A; V179T

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
JDW 836 (pCS2-V5-mScarlet-I-hsKRAS4A-WT)

Plasmid

#156411

Purpose

pCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4A

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4A-WT (KRAS Human)

Use

Tags

mScarlet-I

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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