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ArpC2_pLib

Plasmid

#173679

Purpose

ArpC2 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

ArpC2 (ARPC2 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pFIN-RK-GFP-mIRBP156-CHER-WPRE

Plasmid

#44351

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Inserts

RK-GFP
mIRBP156-CHER

Use

Lentiviral

Tags

Expression

Mutation

Promoter

human rhodopsin kinase (pos 2554-2850) and mouse …

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-EGFP-C-IFT46

Plasmid

#218724

Purpose

Expresses N-terminally EGFP-tagged IFT46 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

IFT46 (IFT46 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-IFT25

Plasmid

#218721

Purpose

Expresses N-terminally EGFP-tagged IFT25 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

IFT25 (HSPB11 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-C1-FKBPflex-ELL-CAAX

Plasmid

#220076

Purpose

an extra long linker attached to a CAAX domain with an mCherry tag

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

CAAX (HRAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNB_EA_R1CiM_4EGFP_R3EGFPi2

Plasmid

#185968

Purpose

Molecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.

Depositor

Sangram Bagh

Article

Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-

Insert

Molecular IMPLY gate

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-NHASt-codoptUL33

Plasmid

#135103

Purpose

Codon-optimized HSV-1 UL33 with N-terminal HAStrep tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 UL33 codon optimized

Use

Tags

HA-Strep-Strep

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-codoptUL50-CStHA

Plasmid

#135105

Purpose

Codon-optimized HSV-1 UL50 with C-terminal StrepHA tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 UL50 codon optimized

Use

Tags

Strep-Strep-HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-codoptUL33-CStHA

Plasmid

#135106

Purpose

Codon-optimized HSV-1 UL33 with C-terminal StrepHA tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 UL33 codon optimized

Use

Tags

Strep-Strep-HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-codoptUS2-CStHA

Plasmid

#135107

Purpose

Codon-optimized HSV-1 US2 with C-terminal StrepHA tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 US2 codon optimized

Use

Tags

Strep-Strep-HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-Dest47- R5A-K5A N23 C11orf83-GFP

Plasmid

#65846

Purpose

Mammalian expression of the mutated N terminal part (R5A-K5A N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

Transmembrane (23 AA) of UQCC3 (UQCC3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

R5A-K6A

Promoter

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-Dest47- R5D-K6D N23 C11orf83-GFP

Plasmid

#65847

Purpose

Mammalian expression of the mutated N terminal part (R5D-K5D N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

Transmembrane (23 AA) of UQCC3 (UQCC3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

R5D-K6D

Promoter

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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c-Myc-5-HT2A

Plasmid

#67944

Purpose

N-terminally c-myc tagged human 5-hydroxytryptamine receptor 2A

Depositor

Javier Gonzalez-Maeso

Article

Gonzalez-Maeso et al Nature. 2008 Mar 6;452(7183):93-7. doi:

Insert

HTR2A (HTR2A Human)

Use

Tags

c-Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pFIN-RK-GFP-WPRE

Plasmid

#44358

Depositor

Susan Semple-Rowland

Article

Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Insert

RK-GFP (MAPK14 Human, Synthetic)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

human rhodopsin kinase (pos 2557-2853)

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5FRT/FLAG-Ino80 ΔN (267-1556)

Plasmid

#29440

Depositor

Ronald Conaway, Joan Conaway

Article

Chen et al J Biol Chem. 2011 Feb 8. ():.

Insert

INO80 (INO80 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

INO80 267-1556 (a.a.)

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/FLAG-Ino80 ΔN-EQ (267-1556)

Plasmid

#29438

Depositor

Ronald Conaway, Joan Conaway

Article

Chen et al J Biol Chem. 2011 Feb 8. ():.

Insert

INO80 (INO80 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

INO80 267-1556 (a.a.) E653Q

Promoter

Available since

July 1, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5FRT/Flag-hINO80 ΔN ΔHSA (520-1556)

Plasmid

#29446

Depositor

Ronald Conaway, Joan Conaway

Article

Chen et al J Biol Chem. 2011 Feb 8. ():.

Insert

INO80 (INO80 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

INO80 520-1556 (a.a.)

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/FLAG-Ino80 ΔNC (267-1261)

Plasmid

#29439

Depositor

Ronald Conaway, Joan Conaway

Article

Chen et al J Biol Chem. 2011 Feb 8. ():.

Insert

INO80 (INO80 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

INO80 267-1261 (a.a.) with Q1116K mutation

Promoter

Available since

Aug. 1, 2011

Availability

Academic Institutions and Nonprofits only
ArpC3-FLAG_pLib

Plasmid

#173680

Purpose

ArpC3 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

ArpC3 (ARPC3 Human)

Use

Tags

TEV cleavage site and FLAG tag

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5FRT/FLAG-Ino80 ΔNC-EQ (267-1261)

Plasmid

#29437

Depositor

Ronald Conaway, Joan Conaway

Article

Chen et al J Biol Chem. 2011 Feb 8. ():.

Insert

INO80 (INO80 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

INO80 267-1261 (a.a.) E653Q

Promoter

Available since

July 1, 2011

Availability

Academic Institutions and Nonprofits only