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pLenti PA-mCit-GW

Plasmid

#113456

Purpose

Lentiviral ProteinA-mCitrine Gateway shuttle vector for N-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Lentiviral

Tags

ProteinA-mCitrine

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSN65

Plasmid

#100123

Purpose

expresses putative SOX2 DBD with a V5 tag

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCitrine-P2A-putative SOX2 DBD (41-109) with a V5 tag (SOX2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti PA-mCit

Plasmid

#113451

Purpose

Lentiviral ProteinA-mCitrine expression vector

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-mCitrine

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hUBbC

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSN24

Plasmid

#100109

Purpose

expresses a chimeric SOX2-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCitrine-P2A-SOX2 fused to a 'weak' VP16 domain (SOX2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBiex PKC - Peptide 14 mCit mCer

Plasmid

#83565

Purpose

Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

Insert

PKC alpha (PRKCA Human)

Use

Tags

10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…

Expression

Bacterial and Insect

Mutation

Only catalytic domain of PKC (aa 335-672)

Promoter

T7 Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti PA-mCit-NL

Plasmid

#113452

Purpose

Lentiviral ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-mCitrine-NanoLuc

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

hUBbC

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-OptoFGFR1-Y766F

Plasmid

#59777

Purpose

Expresses optoFGFR1 with mutation in PLC-gamma binding phosphotyrosine site of FGFR1

Depositor

Won Do Heo

Article

Kim et al Chem Biol. 2014 Jul 17;21(7):903-12. doi

Insert

OptoFGFR1-Y766F (FGFR1 Human, Mustard Weed)

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-OptoFGFR1-D387A

Plasmid

#59778

Purpose

Expresses optoFGFR1 with mutation in PHR to suppress homointeraction, thus cannot be activated by light

Depositor

Won Do Heo

Article

Kim et al Chem Biol. 2014 Jul 17;21(7):903-12. doi

Insert

OptoFGFR1-D387A (FGFR1 Human, Mustard Weed)

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSN25

Plasmid

#100110

Purpose

expresses a chimeric SOX2-repressor fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCitrine-P2A-SOX2 fused to full length HP1 alpha (SOX2, CBX5 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PA-mCit-BAD

Plasmid

#113459

Purpose

ProteinA-mCitrine-BAD expression vector (positive control together with pcDNA3.1 NL-BCL2L1)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

BCL2 associated agonist of cell death (BAD Human)

Use

Tags

ProteinA-mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pRSETA_ionRD

Plasmid

#172924

Purpose

Ionic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.

Depositor

Arnold Boersma

Article

Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

Insert

IonicStrengthSensorRD

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pRSETA_ionRE

Plasmid

#172926

Purpose

Ionic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.

Depositor

Arnold Boersma

Article

Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

Insert

IonicStrengthSensorRE

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pRSETA_ionKE

Plasmid

#172925

Purpose

Ionic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.

Depositor

Arnold Boersma

Article

Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

Insert

IonicStrengthSensorKE

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_crGE

Plasmid

#172933

Purpose

Crowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.

Depositor

Arnold Boersma

Article

Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

Insert

CrowdingSensorGE

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pRSETA_crGE

Plasmid

#172927

Purpose

Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.

Depositor

Arnold Boersma

Article

Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

Insert

CrowdingSensorGE

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ionRD

Plasmid

#172931

Purpose

Ionic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.

Depositor

Arnold Boersma

Article

Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

Insert

IonicStrengthSensorRD

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_ionRE

Plasmid

#172932

Purpose

Ionic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.

Depositor

Arnold Boersma

Article

Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

Insert

IonicStrengthSensorRE

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCY 3080-04

Plasmid

#36228

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

TRP1

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-yEmCitrine-TRP1

Expression

Mutation

Promoter

Available since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pCY 3080-06

Plasmid

#36230

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

URA3

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-yEmCitrine-URA3

Expression

Mutation

Promoter

Available since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pCY 3120-04

Plasmid

#36238

Depositor

Anne Robinson

Article

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

TRP1

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-yECitrine-13Myc-TRP1

Expression

Mutation

Promoter

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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