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Showing: 501 - 520 of 8355 results

  3. pCAG-T3-CjCAS9-pA

    Plasmid
    #131543
    Purpose
    Expresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys
    Depositor
    Wataru Fujii
    Article
    Fujii et al Int J Mol Sci. 2017 Oct 31;18(11). pii:
    Insert
    CjCas9
    Use
    CRISPR
    Tags
    FLAG-SV40NLS, SV40NLS, and polyA
    Expression
    Mammalian
    Mutation
    Human codon-optimized
    Promoter
    CAG, T3
    Available since
    Nov. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    131543-plasmid-map-sequence-id-262486

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  4. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA

    Plasmid
    #136659
    Purpose
    Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD9:TH1477 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136659-plasmid-map-sequence-id-273359

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  9. pCAGGS_m-AKAR5’

    Plasmid
    #114140
    Purpose
    Mammalian expression of the AKAR5’ sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    AKAR5' C-terminally tagged by KRas C tail
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    114140-plasmid-map-sequence-id-215469

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  10. Gli3ZF in AINGpCAGGS

    Plasmid
    #121972
    Purpose
    Gli3 zinc fingers
    Depositor
    James Briscoe
    Insert
    Gli3ZF (GLI3 Human)
    Use
    Chick electroporation
    Tags
    5xmyc
    Expression
    Mammalian
    Mutation
    N terminus and C terminus deletion only nt 1411-1…
    Promoter
    Available since
    April 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    121972-plasmid-map-sequence-id-241924

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  11. pCAG-EHC

    Plasmid
    #112619
    Purpose
    expression plasmid with CAG promoter driving multicistronic cassette H2BEmerald_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-Emerald (H2BC3 Aequorea victoria, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    112619-plasmid-map-sequence-id-226490

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  13. pCAG-TALEN-A-pA

    Plasmid
    #45955
    Depositor
    Ralf Kuehn
    Insert
    TALEN
    Use
    Tags
    FokI nuclease domain
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    45955-plasmid-map-sequence-id-64548

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  14. pCAG-TALEN-T-pA

    Plasmid
    #45963
    Depositor
    Ralf Kuehn
    Insert
    TALEN
    Use
    Tags
    FokI nuclease domain
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    45963-plasmid-map-sequence-id-64556

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  15. pCAG-TALEN-G-pA

    Plasmid
    #45962
    Depositor
    Ralf Kuehn
    Insert
    TALEN
    Use
    Tags
    FokI nuclease domain
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 14, 2013
    Availability
    Academic Institutions and Nonprofits only
    45962-plasmid-map-sequence-id-64555

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Showing: 501 - 520 of 8355 results